Genome size variation and morphological differentiation within Ranunculus parnassifolius group (Ranunculaceae) from calcareous screes in the Northwest of Spain

Cires, Eduardo; Cuesta, Candela; Peredo, Elena L.; Revilla, M. A.; Prieto, José Antonio Fernández

doi:10.1007/s00606-009-0201-9

Cited by 41 publications

(64 citation statements)

References 53 publications

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“…In this respect, our results differ from those of Popp et al (2008) and Dušková et al (2010), who successfully calculated relative DNA content from dried tissues, although this divergence probably lies in different fluorochromes used [4',6-diamidino- 2-phenylindole (DAPI) versus propidium iodide]. Temsch and Greilhuber (2010) considered that the C-values obtained for dried material of Knautia ambigua should be regarded as preliminary, since HPCVs were above the acceptable 5% (Suda and Trávníček 2006;Greilhuber et al 2007), and Cires et al (2009) noted that measurements in dry leaves resulted in lower yields of intact nuclei and coefficient of variation (HPCV) slightly higher than in fresh tissues. Despite obtaining significantly different C-value estimations for fresh and desiccated material, Bainard et al (2011) regarded tissue treatment as one of the many factors that potentially interfere in flow cytometry genome size estimation, accounting for a variation (up to 9.7%) they considered as an acceptable error margin.…”

Section: Discussioncontrasting

confidence: 84%

“…Despite obtaining significantly different C-value estimations for fresh and desiccated material, Bainard et al (2011) regarded tissue treatment as one of the many factors that potentially interfere in flow cytometry genome size estimation, accounting for a variation (up to 9.7%) they considered as an acceptable error margin. Although the effectiveness of flow cytometric analysis for genome size determination rapidly decreases with storage time, it should still remain suitable for-at least-ploidy level determination (Suda and Trávníček 2006;Cires et al 2009). It is noteworthy that, in our study, HPCV from silica gel measurements, although below 5%, do not necessary have a corresponding accurate DNA content, suggesting that HPCV might not be a good quality indicator for this type of material.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, other parameters, such as leaf age and/or its position on the plant, can lead to variations in DNA amount measurements (Palomino et al 2003), which are probably in part explained by differences in secondary metabolism. Protocols for eluding the fresh material requirement by the use of frozen or dehydrated tissues (silica gel-preserved or herbarium vouchers) are receiving increased attention (Suda and Trávníček 2006;Popp et al 2008;Cires et al 2009;Dušková et al 2010;Temsch and Greilhuber 2010;Bainard et al 2011), although their accuracy for drawing comparisons between individuals of close genome sizes is still not clear (Cires et al 2009). …”

Section: Introductionmentioning

confidence: 99%

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Genome size and chromosome number in Echinops (Asteraceae, Cardueae) in the Aegean and Balkan regions: technical aspects of nuclear DNA amount assessment and genome evolution in a phylogenetic frame

et al. 2012

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This work focuses on the representatives of genus Echinops (Asteraceae, Cardueae) in the Aegean and Balkan regions, from the perspective of their genome evolution. Chromosome numbers were determined by orcein staining in 14 populations of nine taxa, and DNA contents were assessed by flow cytometry in 24 populations of nine taxa. A molecular phylogeny based on the internal transcribed spacer (ITS) and trnL-trnF and including first sequences for two taxa (Echinops sphaerocephalus subsp. taygeteus and E. spinosissimus subsp. neumayeri) provided a framework for discussing genome changes. From a methodological point of view, similar C-DNA value estimates were obtained when measuring, for a same population, fresh leaves from adult plants collected in the field and from cultivated seedlings. Conversely, despite giving the appearance of being correct (e.g., low coefficient of variation), genome size assessed using silica gel-preserved material differs significantly from values obtained for the same populations with fresh material. Nevertheless, silica gel-preserved material may still provide rough estimates of genome size for, e.g., inferring ploidy level. Suitablenon-silica gel-based-DNA amounts assessed for 23 populations range from 2C = 6.52 pg (E. spinosissimus subsp. neumayeri) to 2C = 9.37 pg (E. bannaticus). Chromosome counts were established for the first time for Echinops graecus (2n = 32), E. sphaerocephalus subsp. albidus (2n = 32), E. sphaerocephalus subsp. taygeteus (2n = ca. 30), and E. spinosissimus subsp. neumayeri (2n = 28). Genome size and chromosome number are confirmed as crucial parameters for deciphering lineage diversification within the genus Echinops.

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Section: Discussioncontrasting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome size and chromosome number in Echinops (Asteraceae, Cardueae) in the Aegean and Balkan regions: technical aspects of nuclear DNA amount assessment and genome evolution in a phylogenetic frame

et al. 2012

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“…This has included geographic surveys (e.g., Šmarda and Stančík 2006;Whittemore and Olsen 2011), phylogeographic studies (Eidesen et al 2007;Schönswetter et al 2007;Suda et al 2007;Popp et al 2008), and studies of the evolution of polyploid complexes (Harbaugh 2008;Balao et al 2009;Hersch-Green and Cronn 2009;Dušková et al 2010;Sonnleitner et al 2010). At the same time, the practical advantages of the method have led researchers to extend its use beyond ploidy analysis into reports of absolute genome size (e.g., Little et al 2007;Cires et al 2009;Whittemore and Olsen 2011) and fine-scale comparisons among populations or species of the same ploidy Popp et al 2008;Hersch-Green and Cronn 2009;Balao et al 2009;Dušková et al 2010).…”

Section: Introductionmentioning

confidence: 96%

The effects of rapid desiccation on estimates of plant genome size

et al. 2011

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Flow cytometry has become the dominant method for estimating nuclear DNA content in plants, either for ploidy determination or quantification of absolute genome size. Current best practices for flow cytometry involve the analysis of fresh tissue, however, this imposes significant limitations on the geographic scope and taxonomic diversity of plants that can be included in large-scale genome size studies. Dried tissue has been used increasingly in recent years, but largely in the context of ploidy analysis. Here we test rapid tissue drying with silica gel as a method for use in genome size studies, potentially enabling broader geographic sampling of plants when fresh tissue collection is not feasible. Our results indicate that rapid drying introduces comparatively minor error (<10%), which is similar to the error introduced by other common methodological variations such as instrument. Additionally, the relative effect of drying on genome size and data quality varied between species and buffers. Tissue desiccation provides a promising approach for expanding our knowledge of plant genome size diversity.

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“…Morphology has always been the first taxonomic evidence for the recognition and delimitation of infraspecific categories. The key role of morphology in defining infraspecific taxa is shown by the fact that no formal taxonomic status would be assigned when there is no morphological differences among populations of particular plant species (Cires et al, 2009;Lohwasser et al, 2010).…”

Section: Ajabsmentioning

confidence: 99%

ASSESSMENT OF TAXONOMIC AFFINITY OF INDONESIAN PUMMELO (<i>CITRUS MAXIMA</i> (BURM.) MERR.) BASED ON MORPHOLOGICAL CHARACTERS

Susandarini

Subandiyah

Rugayah³

et al. 2013

American Journal of Agricultural and Biological Sciences

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Citrus maxima is a tropical fruit species with high morphological variability on fruit characters, including shape, size, thickness of peel, flesh color and taste. This variability has been used by breeders and common people to practically distinguish different pummelo cultivars. Despite such well known variation, so far there is no published study specifically addressed the extent of phenotypic variability and taxonomy of pummelo in Indonesia. This study was, therefore, aiming at assessing phenotypic variability and taxonomic affinity of Indonesian pummelo cultivars and landraces using multivariate analysis methods. A total of 60 accessions from six provinces were collected, including some representatives of registered cultivars. Twenty one morphological characters were subjected to cluster analysis and principal component analysis. Results showed that two major clusters were formed, in which each group was characterized by combination of distinctive morphological features leading to recognition of two cultivar groups. Based on the fact that the morphology of individuals from intensively cultivated orchards showed similar level of variability to those from sporadically distributed areas, there was strong indication that the morphological variation has genetic background.

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Genome size variation and morphological differentiation within Ranunculus parnassifolius group (Ranunculaceae) from calcareous screes in the Northwest of Spain

Cited by 41 publications

References 53 publications

Genome size and chromosome number in Echinops (Asteraceae, Cardueae) in the Aegean and Balkan regions: technical aspects of nuclear DNA amount assessment and genome evolution in a phylogenetic frame

Genome size and chromosome number in Echinops (Asteraceae, Cardueae) in the Aegean and Balkan regions: technical aspects of nuclear DNA amount assessment and genome evolution in a phylogenetic frame

The effects of rapid desiccation on estimates of plant genome size

ASSESSMENT OF TAXONOMIC AFFINITY OF INDONESIAN PUMMELO (<i>CITRUS MAXIMA</i> (BURM.) MERR.) BASED ON MORPHOLOGICAL CHARACTERS

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