Genome Sequence of a Tomato-Infecting Tomato Mosaic Virus Isolate from Zimbabwe

Karavina, Charles; Ibaba, Jacques Davy; Gubba, Augustine

doi:10.1128/genomea.01457-17

Cited by 3 publications

(1 citation statement)

References 7 publications

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“…It is di cult to control the spread of ToMV as it can be transmitted in seeds or through mechanical inoculation, contaminated tools or workers [2,12,13]. Within the three pathological groups of different ToMV populations, global ToMV isolates in Group I mostly infected solanaceous host species tested while the isolates in Group II did also infect other plants [18,19].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of CMV and ToMV Isolates in Tomato and Pepper Production Areas in Hakkari, Turkey

AKDURA

Kiliç

2022

Preprint

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Background Tomato (Lycopersicum esculentum Mill.) and pepper (Capsicum anuum L.) are the host species for the Cucumber mosaic virus (CMV) and Tomato mosaic virus (ToMV). This article aims to report the detection of serolocigally and molecularly and identification of genetic diversity these viruses in Hakkari province, Turkey.Methods and results A total of 184 leaf samples were collected from tomato and pepper plants that showed virus-like symptoms. Sample collection was carried out in Hakkari province in October, 2020. CMV and ToMV factors were investigated by Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA). DAS-ELISA test showed that 106 (57.60%) of the samples were infected with one or more viruses. For phylogenetic analyses, 10 samples which were positive for CMV and ToMV were tested using Reverse Transcription Polymerase Chain Reaction (RT-PCR) method. Coat protein (CP) and replicase genes of Hakkari CMV and ToMV isolates were amplified by RT-PCR using specific primers. The resulting DNA sequences were deposited in NCBI GenBank (Accession Nos: OM286731, OM286732, OM286733, OM286734, OM418629). When the replicase and coat protein gene sequences of four ToMV isolates and one CMV isolate were compared with the isolates in GenBank, a nucleotide homology of 98.1-99.6% and 91.7-99.4% were found, respectively. Phylogenetic analysis revealed a strong similarity between Hakkari ToMV and CMV isolates and other Turkish isolates. Consistent with the molecular studies conducted in Turkey on CMV and ToMV, the isolates detected in this study also clustered in subgroup Ia. Conclusions With this study, CMV and ToMV factors were serologically and molecularly detected for the first time in Hakkari and the first nation-wide report for ToMV replicase sequences in infected tomato and peppers were submitted.

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Section: Introductionmentioning

confidence: 99%

Genetic Diversity of CMV and ToMV Isolates in Tomato and Pepper Production Areas in Hakkari, Turkey

AKDURA

Kiliç

2022

Preprint

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Detection and molecular analysis of shallot latent virus infecting Allium sativum in Zimbabwe

Karavina,

Ibaba,

Gubba

2023

Physiological and Molecular Plant Pathology

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High-Throughput Sequencing Application in the Diagnosis and Discovery of Plant-Infecting Viruses in Africa, A Decade Later

Ibaba

Gubba

2020

Plants

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High-throughput sequencing (HTS) application in the field of plant virology started in 2009 and has proven very successful for virus discovery and detection of viruses already known. Plant virology is still a developing science in most of Africa; the number of HTS-related studies published in the scientific literature has been increasing over the years as a result of successful collaborations. Studies using HTS to identify plant-infecting viruses have been conducted in 20 African countries, of which Kenya, South Africa and Tanzania share the most published papers. At least 29 host plants, including various agricultural economically important crops, ornamentals and medicinal plants, have been used in viromics analyses and have resulted in the detection of previously known viruses and novel ones from almost any host. Knowing that the effectiveness of any management program requires knowledge on the types, distribution, incidence, and genetic of the virus-causing disease, integrating HTS and efficient bioinformatics tools in plant virology research projects conducted in Africa is a matter of the utmost importance towards achieving and maintaining sustainable food security.

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Genome Sequence of a Tomato-Infecting Tomato Mosaic Virus Isolate from Zimbabwe

Cited by 3 publications

References 7 publications

Genetic Diversity of CMV and ToMV Isolates in Tomato and Pepper Production Areas in Hakkari, Turkey

Genetic Diversity of CMV and ToMV Isolates in Tomato and Pepper Production Areas in Hakkari, Turkey

Detection and molecular analysis of shallot latent virus infecting Allium sativum in Zimbabwe

High-Throughput Sequencing Application in the Diagnosis and Discovery of Plant-Infecting Viruses in Africa, A Decade Later

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