Genome Sequence of a Neisseria meningitidis Capsule Null Locus Strain from the Clonal Complex of Sequence Type 198

Schork, Sabine; Schlüter, Andreas; Blom, Jochen; Schneiker-Bekel, Susanne; Pühler, Alfred; Goesmann, Alexander; Frosch, Matthias; Schoen, Christoph

doi:10.1128/jb.01099-12

Cited by 8 publications

(5 citation statements)

References 15 publications

(13 reference statements)

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“…S1 in the supplemental material). Prior literature has indicated that, out of cnl meningococcal strains, ST-198 isolates in particular have been able to cause invasive meningococcal disease, despite being unencapsulated (9). The presence of three such unusual isolates in our data set raises the possibility that urethritis is another manifestation of this clade's pathogenicity.…”

Section: Resultsmentioning

confidence: 70%

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Genomic Characterization of Urethritis-Associated Neisseria meningitidis Shows that a Wide Range of N. meningitidis Strains Can Cause Urethritis

et al. 2017

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, typically a resident of the oro- or nasopharynx and the causative agent of meningococcal meningitis and meningococcemia, is capable of invading and colonizing the urogenital tract. This can result in urethritis, akin to the syndrome caused by its sister species, , the etiologic agent of gonorrhea. Recently, meningococcal strains associated with outbreaks of urethritis were reported to share genetic characteristics with the gonococcus, raising the question of the extent to which these strains contain features that promote adaptation to the genitourinary niche, making them gonococcus-like and distinguishing them from other strains. Here, we analyzed the genomes of 39 diverse isolates associated with urethritis, collected independently over a decade and across three continents. In particular, we characterized the diversity of the nitrite reductase gene (), the factor H-binding protein gene (), and the capsule biosynthetic locus, all of which are loci previously suggested to be associated with urogenital colonization. We observed notable diversity, including frameshift variants, in and and the presence of intact, disrupted, and absent capsule biosynthetic genes, indicating that urogenital colonization and urethritis caused by are possible across a range of meningococcal genotypes. Previously identified allelic patterns in urethritis-associated strains may reflect genetic diversity in the underlying meningococcal population rather than novel adaptation to the urogenital tract.

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Section: Resultsmentioning

confidence: 70%

“…1) (13). Three of these isolates (excluding NmJPb05-3) belonged to the ST-198 clade; among the rare cases of unencapsulated N. meningitidis isolates associated with invasive disease, isolates from the ST-198 clade are frequently found (9). Isolates in the ST-198 clade also lacked region B (ctrE, ctrF) genes, whereas NmJPb05-3 possessed them.…”

Section: Resultsmentioning

confidence: 99%

Genomic Characterization of Urethritis-Associated Neisseria meningitidis Shows that a Wide Range of N. meningitidis Strains Can Cause Urethritis

et al. 2017

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“…In contrast to the invasive isolates, only 35 The remainder of the isolates (n ϭ 58) had an incomplete cps locus, with 40% of the isolates missing ctrA. Thirty-nine (67%) of the 58 isolates were ctrA negative; of those, 32 isolates lacked capsule biosynthesis and capsule transport genes and thus were classified as capsule operon-null (cnl) isolates (83,84). Fifteen isolates had insertions bearing coding sequences from IS1301 (80)(81)(82), including nine in cssF (MnW) and three each in cssA (MnY) and csb (MnB).…”

Section: Genotypic Characterization By Wgsmentioning

confidence: 99%

Comparison of Phenotypic and Genotypic Approaches to Capsule Typing of Neisseria meningitidis by Use of Invasive and Carriage Isolate Collections

Mohamed

Rojas

et al. 2016

J Clin Microbiol

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e Neisseria meningitidis serogroup B (MnB) is a leading cause of bacterial meningitis; however, MnB is most commonly associated with asymptomatic carriage in the nasopharyngeal cavity, as opposed to the disease state. Two vaccines are now licensed for the prevention of MnB disease; a possible additional benefit of these vaccines could be to protect against disease indirectly by disrupting nasopharyngeal carriage (e.g., herd protection). To investigate this possibility, accurate diagnostic approaches to characterize MnB carriage isolates are required. In contrast to invasive meningococcal disease (IMD) isolates, which can be readily serogrouped, carriage isolates often lack capsule expression, making standard phenotypic assays unsuitable for strain characterization. Several antibody-based methods were evaluated for their abilities to serogroup isolates and were compared with two genotyping methods (real-time PCR [rt-PCR] and whole-genome sequencing [WGS]) to identify which approach would most accurately ascertain the polysaccharide groups associated with carriage isolates. WGS and rt-PCR were in agreement for 99% of IMD isolates, including those with coding sequences for MnB, MnC, MnW, and MnY, and the phenotypic methods correctly identified serogroups for 69 to 98% of IMD isolates. In contrast, only 47% of carriage isolates were groupable by genotypic methods, due to mutations within the capsule operon; of the isolates identified by genotypic methods, <43% were serogroupable with any of the phenotypic methods tested. These observations highlight the difficulties in the serogrouping and capsular genogrouping of meningococcal carriage isolates. Based on our findings, WGS is the most suitable approach for the characterization of meningococcal carriage isolates. N eisseria meningitidis, a Gram-negative bacterium that causes both epidemic and endemic life-threatening disease, is also an obligate human commensal organism that colonizes the nasopharyngeal mucosa with no or minimal harm to the host, a phenomenon known as carriage (1). Asymptomatic pharyngeal colonization with N. meningitidis in young adults is relatively common, and these asymptomatic carriers represent a potential reservoir for the transmission of pathogenic isolates in the community (2, 3). The rates of asymptomatic carriage in the United States and Europe are highest among adolescents and young adults, peaking at the age of 19 years, and are estimated to be 10% to 35% (4-6). Rates of transmission and carriage are higher in closed and semiclosed populations, such as university students living in dormitories and military recruits housed in barracks (7). Higher rates of carriage are also found among people in close contact with patients with active meningococcal infections (8). For the majority of people, carriage is an immunizing process that results in systemic, serogroup-specific, protective antibody responses (9, 10). Invasive meningococcal disease (IMD) usually occurs shortly after the onset of colonization of a susceptible host, when the bacteria ...

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“…Neisseria meningitidis Capsule Null Locus Strain, sequence accession CAJS01000001 through CAJS01000042 [47]…”

Section: Bacteriamentioning

confidence: 99%

Genome sequences published outside of Standards in Genomic Sciences, July - October 2012

Nelson¹

2012

Stand. Genomic Sci.

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The purpose of this table is to provide the community with a citable record of publications of ongoing genome sequencing projects that have led to a publication in the scientific literature. While our goal is to make the list complete, there is no guarantee that we may have omitted one or more publications appearing in this time frame. Readers and authors who wish to have publications added to subsequent versions of this list are invited to provide the bibliographic data for such references to the SIGS editorial office.

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Genome Sequence of a Neisseria meningitidis Capsule Null Locus Strain from the Clonal Complex of Sequence Type 198

Cited by 8 publications

References 15 publications

Genomic Characterization of Urethritis-Associated Neisseria meningitidis Shows that a Wide Range of N. meningitidis Strains Can Cause Urethritis

Genomic Characterization of Urethritis-Associated Neisseria meningitidis Shows that a Wide Range of N. meningitidis Strains Can Cause Urethritis

Comparison of Phenotypic and Genotypic Approaches to Capsule Typing of Neisseria meningitidis by Use of Invasive and Carriage Isolate Collections

Genome sequences published outside of Standards in Genomic Sciences, July - October 2012

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