Genome Sequence of a Foot-and-Mouth Disease Virus Detected in Indonesia in 2022

Zainuddin, Nuryani; Susila, Edy Budi; Wibawa, Hendra; Daulay, Rosmalina Sari Dewi; Wijayanti, Putriani Endah; Fitriani, Dini; Hidayati, Dewi Noor; Idris, Syafrison; Wadsworth, Jemma; Polo, Noemi; Hicks, Hayley; Mioulet, Valérie; Knowles, Nick J.; King, Donald P.

doi:10.1128/mra.01081-22

Cited by 6 publications

(6 citation statements)

References 10 publications

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“…This issue leads to the importance of developing diagnostic and vaccine strategies based on the location of the virus circulated. Phylogenetic analyses of the VP1-coding region described the sequence of O/ME-SA/Ind-2001e lineage, sharing the closest nucleotide identity to other contemporary sequences collected from Indonesia [6].…”

Section: Discussionmentioning

confidence: 83%

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Expression of VP1 protein of foot-and-mouth disease virus serotype O originated from Indonesia in mammalian cells as potential immunogen

Widayanti,

Suryanggono,

Pambudi

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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In 2022, Indonesia experienced foot-and-mouth disease (FMD) outbreak resulting in the death of hundreds of cattle; after more than 30 years, the country maintained FMD-free status. The FMD viral polypeptides-1 (VP1) protein contains major antigenic sites, making the protein have an important role in diagnostic applications or vaccine development. In this study, a synthetic codon-optimized vp1 gene from FMD virus serotype O was integrated into pcDNA3.1 vector and transformed to Escherichia coli TOP10F’ for plasmid propagation. The pcDNA3.1-1D-VP1 FMDV plasmid was then verified using agarose gel, showing in 659 bp size of the DNA fragment. The VP1 recombinant plasmid was transfected into HEK293T cells in DMEM medium supplemented with 10% FBS. Protein profiles were determined with SDS-PAGE showing target protein at 33KDa. Protein expression was confirmed by in-cell western assay using anti-VP1 type O polyclonal antibody and IRDye® 800CW goat anti-rabbit IgG as the secondary antibody. The result revealed a high-intensity fluorescence signal, indicating a strong interaction between expressed protein and anti-VP1 antibody. Thus, the results of this study demonstrate the potential for VP1 protein to be used as an immunogen in vaccine or diagnostic development against FMD infection. Nonetheless, some additional studies should be conducted.

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Section: Discussionmentioning

confidence: 83%

“…FMD was reported in Indonesia in the mid-year of 2022, after IOP Publishing doi:10.1088/1755-1315/1271/1/012094 2 almost 40 years the country had not experienced an FMD outbreak. There have been reported that all the FMD cases in Indonesia are identified as FMDV type O [6].…”

Section: Introductionmentioning

confidence: 99%

Expression of VP1 protein of foot-and-mouth disease virus serotype O originated from Indonesia in mammalian cells as potential immunogen

Widayanti,

Suryanggono,

Pambudi

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…The FMD causes a significant loss in livestock production as it causes the loss of weight, reproductive disturbances, and in the worst case, death [1]. In 2022, Indonesia had a FMD outbreak after 35 years of free cases of FMD [2]. The outbreak was first reported in East Java and then spread in other regions [3].…”

Section: Introductionmentioning

confidence: 99%

“…Due to its indispensable role in augmenting viral replication and harbouring host's immune response, pharmaceutical agents have been designed to target 3CP [9,15]. Moreover, Indonesian serotype is very exclusive phylogenetically compared to another O serotype across Asiatic region [2]. With that uniqueness along with no information about the effect of natural compounds in inhibiting FMDV replication, particularly Indonesian serotype, underscores an avenue for exploration that could potentially enhance the arsenal against FMDV infections.…”

Section: Introductionmentioning

confidence: 99%

Beyond the Magic of Moringa oleifera: Its Potential to Control Indonesian Serotype of Footand-Mouth-Disease Virus Replication through Inhibition of 3-Cysteine Protease

Kusuma,

Widyananda,

Grahadi

et al. 2024

BIO Web Conf.

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Foot-and-Mouth Disease (FMD) poses a significant threat to livestock worldwide, necessitating innovative approaches to combat its causative agent, the FMD virus (FMDV). On the other hand, Moringa oleifera is a feed alternative for cattles with numerous bioactive compounds. This paper delves into the captivating realm of Moringa oleifera (MO) bioactives and their potential in thwarting FMDV replication by targeting the essential enzyme, 3C Protease (3CP). To elucidate the inhibitory potential of these bioactives, a rigorous investigation involving molecular docking and molecular dynamics simulations was conducted. Specifically, the 3CP was modeled based on the amino acid sequence of FMDV Indonesian Serotype. Results showed that most of the compounds from MO outperformed Ribavirin as the standard therapy for FMD. Among them, Baicalin, Chlorogenic Acid, and Rutin have binding affinity -9.1, -8.1, and -8.1 kcal/mol, respectively. Those compounds also formed more hydrogen bonds than Ribavirin through their binding sites. Molecular dynamics simulation also revealed that interaction of 3CP with those compounds had minor influence on its structural stability. The conformation of those compounds is also more stable than Ribavirin, supported by more hydrogen bonds. In summary, this research highlighted the potential mechanism of MO bioactives in preventing severe FMDV infection through inhibition of viral replication.

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“…The first FMD vaccination program was an emergency response that was devoted to the dairy cattle population and government breeding centers such as the National Artificial Insemination Centre (NAIC), Singosari which was mandated to produce frozen semen in Indonesia (Zainuddin et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Effect of Foot and Mouth Diseases Vaccination on Basic Semen Quality Parameter in Bali Cattle

Prihatin,

Amaliya,

Musaffak

et al. 2023

J. Med. Vet.

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This study aimed to determine the effect of post foot and mouth diseases (FMD) vaccination on the basic semen quality in Bali cattle bulls. The data used in this study were semen collection data of 25 Bali cattle bulls at the National Artificial Insemination Centre (NAIC), Singosari. The paired sample t-test was performed to compare the response variable of individual motility (%), abnormality (%), spermatozoa concentration (x 106 cells), and semen volume (mL) from regular semen collection in post and pre-vaccination groups. The results showed no significant difference in semen individual motility (t(74) = -1.72, p = 0.95), abnormality (t(74) = 2.06, p = 0.98), spermatozoa concentration (t(74) = -4.35, p = 1.00), and semen volume (t(74) = -0.11, p = 0.54) in Bali cattle bulls before and after vaccination. This study concludes that the FMD's vaccination program for Bali cattle bulls did not have the potential to reduce the basic semen quality for Bali Cattle frozen semen production.

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Genome Sequence of a Foot-and-Mouth Disease Virus Detected in Indonesia in 2022

Cited by 6 publications

References 10 publications

Expression of VP1 protein of foot-and-mouth disease virus serotype O originated from Indonesia in mammalian cells as potential immunogen

Expression of VP1 protein of foot-and-mouth disease virus serotype O originated from Indonesia in mammalian cells as potential immunogen

Beyond the Magic of Moringa oleifera: Its Potential to Control Indonesian Serotype of Footand-Mouth-Disease Virus Replication through Inhibition of 3-Cysteine Protease

Effect of Foot and Mouth Diseases Vaccination on Basic Semen Quality Parameter in Bali Cattle

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