Genome sequence and characterization of a new virus infecting Mikania micrantha H.B.K.

Abstract

The complete RNA genomic sequence of a new virus infecting Mikania micrantha, designated as Mikania micrantha wilt virus (MMWV), has been determined. The genomic sequence and the predicted gene products of MMWV were similar to those of the other viruses of the genus Fabavirus. The MMWV nucleotide sequence showed 75.6% identity to that of gentian mosaic virus, 56.6 and 57% identity to those of two Broad bean wilt virus 1 isolates, and between 55.7 and 58% identity to those of seven Broad bean wilt virus 2 isolates. Our results suggested that MMWV represents a distinct isolate of the candidate species Gentian mosaic virus.

Digital Gene Expression Analysis Based on Integrated De Novo Transcriptome Assembly of Sweet Potato [Ipomoea batatas (L.) Lam.]

“…MMWV was only reported in Mikania micrantha H.B.K. in Guangdong province, China [78]. During the last decade, 11 viruses were reported in China [75], but to our knowledge, this is the first time to detect so many viruses in one sweet potato variety from one field.…”

Effects ofMikania micrantha wilt virusinfection on photosynthesis and the activities of four enzymes inMikania micranthaH.B.K.

“…Ellison 等 [32] 发现薇甘菊柄锈菌 Puccinia spegazzinii 仅侵染薇甘菊属植物。 安婀珍蝶( Actinote anteas) [33] 、紫红 短须螨 ( Brevipalpus phoenicis ) [34] 和 小 蓑 蛾 ( Acanthophyche sp ) [35] 等 可 取 食 薇 甘 菊 的 茎 叶。 田 野 菟 丝 子 ( Cuscuta campestris) 可 控 制 薇 甘 菊 生 长, 但 不 能 根 除 薇 甘 菊 [11] 。 生 态 控 制 目 前 主 要 是 利 用 幌 伞 枫 (Heteropanax fragrans) [11] 和芒萁( Dicranopteris pedata) [36] 等本地物种控制薇甘菊的生长。 发现和利用天敌控 制外来入侵生物作为新兴的方法值得做深入的研究 [16] 。 当前,薇甘菊的防治仍是世界性难题,研究所发现的天敌虽可使薇甘菊致病甚至死亡,但在实际 应用中控制薇甘菊的扩散尚未见报道 [11,15,37] 。 本研究表明 MMWV 通过降低薇甘菊叶片的光合特性和与抗病性相关的酶活性,从而抑制薇甘菊的生长。…”

Detection and identification of Fabavirus species by one-step RT-PCR and multiplex RT-PCR

“…BBWV-1 and BBWV-2 are distributed worldwide (Ferrer et al, 2007), while LMMV has been reported in the United Kingdom only (Lisa et al, 1982); GeMV has been found in Japan and China (Kobayashi et al, 2005;Wang et al, 2008), and CuMMV was reported recently in China (Dong et al, 2012). The availability of sensitive and rapid techniques for the detection and identification of the different fabavirus species is crucial to study their epidemiology and to implement effective disease control strategies.…”

“…The primers for multiplex RT-PCR were designed based on: (1) nucleotide sequences specific to each virus, but conserved between isolates; (2) compatibility between primers so that they could be used in the same RT-PCR; and (3) differences in RT-PCR product size from each virus, so that they could be identified by electrophoresis. The design process to obtain specific primers for BBWV-1, BBWV-2 and GeMV was very complex due to the high genetic variability of these viruses (Kobayashi et al, 1999a(Kobayashi et al, ,b, 2005Ferrer et al, 2005Ferrer et al, , 2011Wang et al, 2008) in comparison with most other plant viruses (García-Arenal et al, 2001;Rangel et al, 2011;Tiberini et al, 2011;Davino et al, 2012). The primers were designed to ensure that the expected sizes of the RT-PCR products were approximately 622, 510, 370, 238 and 98 bp for LMMV, BBWV-1, GeMV, BBWV-2 and CuMMV, respectively (Table 2).…”