Genome-Scale Characterization of Fungal Phytases and a Comparative Study Between Beta-Propeller Phytases and Histidine Acid Phosphatases

Ferreira, Roberta Corsino; Tavares, Murillo Peterlini; Morgan, Túlio; Clevelares, Yan da Silva; Rodrigues, Marina Quádrio Raposo Branco; Kasuya, Maria Catarina Megumi; Mendes, Tiago Antônio de Oliveira; Guimarães, Valéria Monteze

doi:10.1007/s12010-020-03309-7

Cited by 2 publications

(1 citation statement)

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“…Fungal species from the Fusarium species complex produce phosphatases with significant phytase activity ( Marlida et al, 2010 ; Gontia-Mishra et al, 2014 ; Ferreira et al, 2020 ). Some phytase-producing Fusarium species are important pathogens of crop plants.…”

Section: Introductionmentioning

confidence: 99%

Barley Nepenthesin-Like Aspartic Protease HvNEP-1 Degrades Fusarium Phytase, Impairs Toxin Production, and Suppresses the Fungal Growth

et al. 2021

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Nepenthesins are categorized under the subfamily of the nepenthesin-like plant aspartic proteases (PAPs) that form a distinct group of atypical PAPs. This study describes the effect of nepenthesin 1 (HvNEP-1) protease from barley (Hordeum vulgare L.) on fungal histidine acid phosphatase (HAP) phytase activity. Signal peptide lacking HvNEP-1 was expressed in Pichia pastoris and biochemically characterized. Recombinant HvNEP-1 (rHvNEP-1) strongly inhibited the activity of Aspergillus and Fusarium phytases, which are enzymes that release inorganic phosphorous from phytic acid. Moreover, rHvNEP-1 suppressed in vitro fungal growth and strongly reduced the production of mycotoxin, 15-acetyldeoxynivalenol (15-ADON), from Fusarium graminearum. The quantitative PCR analysis of trichothecene biosynthesis genes (TRI) confirmed that rHvNEP-1 strongly repressed the expression of TRI4, TRI5, TRI6, and TRI12 in F. graminearum. The co-incubation of rHvNEP-1 with recombinant F. graminearum (rFgPHY1) and Fusarium culmorum (FcPHY1) phytases induced substantial degradation of both Fusarium phytases, indicating that HvNEP-1-mediated proteolysis of the fungal phytases contributes to the HvNEP-1-based suppression of Fusarium.

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Section: Introductionmentioning

confidence: 99%