Genome-Scale Analysis of the WRI-Like Family in Gossypium and Functional Characterization of GhWRI1a Controlling Triacylglycerol Content

Zang, Xinshan; Pei, Wenfeng; Wu, Man; Geng, Yushui; Wang, Nuohan; Liu, Guoyuan; Ma, Jianjiang; Li, Dan; Cui, Yupeng; Li, Xingli; Zhang, Jinfa; Yu, Jie-Ae

doi:10.3389/fpls.2018.01516

Cited by 13 publications

(7 citation statements)

References 39 publications

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“…WRI1 affects seed oil production in plants of Zea mays (Pouvreau et al 2011), Brassica napus (Liu et al 2010;Wu et al 2014) Brachypodium distachyon (Yang et al 2015), Brassica juncea (Bhattacharya et al 2016) and Lepidium campestre (Ivarson et al 2017). Zang et al (2018) have conducted genome wide identification of wrinkled1 genes from G. hirsutum, G. barbadense, G. arboreum and G. raimondii species and have confirmed that the ectopic expression of wri1a gene from G. hirsutum can rescue the seed phenotype in wri1-7 mutant of A. thaliana. G. hirsutum was transformed to produce Ghwri1 over expressing lines and showed enhanced lipid content in seeds (Liu et al 2018).…”

Section: Introductionmentioning

confidence: 89%

Ectopic Expression of Fiber Related Gbwri1 Complements Seed Phenotype in Arabidopsis thaliana

Imran¹

2021

IJAB

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WRINKLED1 belongs to AP2/EREB family of transcription factors whose role has been well established in seed oil biosynthesis. The objective of the study was to trace the role of fiber related Gbwri1 in seed development and fatty acid biosynthesis. In this study, we isolated a transcript from elite fiber producing cotton (Gossypium barbadense), which is over-expressed in G. barbadense fibers as compared to G. hirsutum and G. arboreum. The putative protein encoded by this transcript exhibited homology in specific domains and protein structure with WRINKLED1 of Arabidopsis thaliana and was thus designated as Gbwri1. In this study, we investigated the functional homology of fiber elongation related Gbwri1 with fatty acid biosynthesis regulator Atwri1. Ectopic expression of Gbwri1 in wri1-3 mutant of A. thaliana was analyzed. In the transgenic lines of A. thaliana, Gbwri1 resumed the seed weight, seed area, and surface morphology to the wild type. Gbwri1 transformation rescued the wrinkled phenotype of wri1-3 mutants by resuming the expression of fatty acid biosynthesis genes biotin carboxyl carrier protein isoform 2 (bccp2) and keto-ACP synthase 1 (kas1). Moreover, the seedling development of transgenic lines on non-sucrose medium demonstrated that the Gbwri1 was able to regulate the supply of sucrose for normal seedling establishment. Our results showed that the transformation of Gbwri1 in A. thaliana wri1-3 mutant was able to complement wri1-3 impaired phenotype. Thus, Gbwri1 is involved in cotton fiber development and fatty acid biosynthesis in seeds. © 2021 Friends Science Publishers

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Section: Introductionmentioning

confidence: 89%

Ectopic Expression of Fiber Related Gbwri1 Complements Seed Phenotype in Arabidopsis thaliana

Imran¹

2021

IJAB

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“…Transgenic plant generation and expression analysis were performed as previously reported [24]. Briefly, complete coding sequence of GhPDAT1d (Additional file 4) was amplified with gene specific primers from G. hirsutum acc.…”

Section: Methodsmentioning

confidence: 99%

“…Total oil content was determined with about 0.3 g seeds per sample using an NMI20-Analyst nuclear magnetic resonance spectrometer (Niumag, Shanghai, China) as previously reported [24].…”

Section: Methodsmentioning

confidence: 99%

A genome-wide analysis of the phospholipid: diacylglycerol acyltransferase gene family in Gossypium

Zang

Geng

et al. 2019

BMC Genomics

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Background Cotton ( Gossypium spp.) is the most important natural fiber crop worldwide, and cottonseed oil is its most important byproduct. Phospholipid: diacylglycerol acyltransferase (PDAT) is important in TAG biosynthesis, as it catalyzes the transfer of a fatty acyl moiety from the sn -2 position of a phospholipid to the sn -3 position of sn -1, 2-diacylglyerol to form triacylglycerol (TAG) and a lysophospholipid. However, little is known about the genes encoding PDATs involved in cottonseed oil biosynthesis. Results A comprehensive genome-wide analysis of G. hirsutum , G. barbadense , G. arboreum , and G. raimondii herein identified 12, 11, 6 and 6 PDAT s, respectively. These genes were divided into 3 subfamilies, and a PDAT-like subfamily was identified in comparison with dicotyledonous Arabidopsis . All GhPDATs contained one or two LCAT domains at the C-terminus, while most GhPDATs contained a preserved single transmembrane region at the N-terminus. A chromosomal distribution analysis showed that the 12 GhPDAT genes in G. hirsutum were distributed in 10 chromosomes. However, none of the GhPDATs was co-localized with quantitative trait loci (QTL) for cottonseed oil content, suggesting that their sequence variations are not genetically associated with the natural variation in cottonseed oil content. Most GhPDATs were expressed during the cottonseed oil accumulation stage. Ectopic expression of GhPDAT1d increased Arabidopsis seed oil content. Conclusions Our comprehensive genome-wide analysis of the cotton PDAT gene family provides a foundation for further studies into the use of PDAT genes to increase cottonseed oil content through biotechnology. Electronic supplementary material The online version of this article (10.1186/s12864-019-5728-8) contains supplementary material, which is available to authorized users.

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“…Studies in rapeseed have shown that overexpression of BnWRI1 not only increases the SOC and seed mass in mature seed, but also improves chlorophyll content in developing seeds (Wu et al., 2014). GhWRI1a and GhWRI1b from cotton were highly expressed in 25‐DAP (days after pollination) developing fibers, suggesting that these two genes participate in fiber development and oil seed content (Zang et al., 2018). Knockdown of GhWRI1a expression in cotton has been found to reduce oil seed content and increase fiber length (Qaisar et al., 2017; Qu et al., 2012).…”

Section: Transcription Factors Regulating the Seed Oil Pathway In Maj...mentioning

confidence: 99%

Multi‐omics‐driven advances in the understanding of triacylglycerol biosynthesis in oil seeds

Li,

Che,

Zhu

et al. 2023

The Plant Journal

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SUMMARYVegetable oils are rich sources of polyunsaturated fatty acids and energy as well as valuable sources of human food, animal feed, and bioenergy. Triacylglycerols, which are comprised of three fatty acids attached to a glycerol backbone, are the main component of vegetable oils. Here, we review the development and application of multiple‐level omics in major oilseeds and emphasize the progress in the analysis of the biological roles of key genes underlying seed oil content and quality in major oilseeds. Finally, we discuss future research directions in functional genomics research based on current omics and oil metabolic engineering strategies that aim to enhance seed oil content and quality, and specific fatty acids components according to either human health needs or industrial requirements.

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Genome-Scale Analysis of the WRI-Like Family in Gossypium and Functional Characterization of GhWRI1a Controlling Triacylglycerol Content

Cited by 13 publications

References 39 publications

Ectopic Expression of Fiber Related Gbwri1 Complements Seed Phenotype in Arabidopsis thaliana

Ectopic Expression of Fiber Related Gbwri1 Complements Seed Phenotype in Arabidopsis thaliana

A genome-wide analysis of the phospholipid: diacylglycerol acyltransferase gene family in Gossypium

Multi‐omics‐driven advances in the understanding of triacylglycerol biosynthesis in oil seeds

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