Genome Profiling for Health Promoting and Disease Preventing Traits Unraveled Probiotic Potential of Bacillus clausii B106

Kapse, Neelam; Engineer, A.S; Gowdaman, V.; Wagh, Shilpa A.; Dhakephalkar, Prashant K.

doi:10.4014/mbl.1804.04001

Cited by 6 publications

(8 citation statements)

References 54 publications

(60 reference statements)

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“…Studies characterizing the mechanism of antibiotic resistance across the O/C, N/R, SIN and T strains of B. clausii have demonstrated the presence of chromosomal sequences conferring resistance to aminoglycosides [ 42 ], macrolides, lincosamides and streptogramin B [ 43 ], chloramphenicol [ 44 ], and rifampicin and streptomycin [ 40 ]. Findings describing similar identified genomic features have also been reported following genome profiling of the B106 strain of B. clausii [ 45 ]. It is noteworthy that resistance of B. clausii strains O/C, N/R, SIN and/or T to the antibiotics described above could not be transferred by conjugation to other bacterial species [ 42 – 44 ].…”

Section: Resultssupporting

confidence: 66%

“…Multiple strains of B. clausii , including O/C, N/R, SIN and T and reference strains DSM 8716 T , DSM 9783 and DSM 2512, exhibit inhibitory activity against different genotoxins, restricted to vegetative cells and not evident with spore suspensions [ 62 ]. As described above, genome profiling of the B106 strain of B. clausii by Kapse et al [ 45 ] identified the presence of numerous genes encoding bacteriocins, supporting an antimicrobial activity of this strain. In contrast to these studies, Duc et al [ 46 ] reported little evidence of bacteriocin production by B. clausii (O/C, N/R, SIN and T) following screening assays with 23 indicator strains that included both gram-positive and gram-negative organisms.…”

Section: Resultsmentioning

confidence: 81%

“…Findings from these studies show the ability of B. clausii spores to survive, germinate and grow after acid and bile salt challenge and under restricted oxygen levels, with some evidence of variation in responses between the different strains [ 47 ]. Separately, strain B106 is likely to exhibit such properties based on the presence of genes encoding proteins related to tolerance of acids, bases and bile salts [ 45 , 50 ].…”

Section: Resultsmentioning

confidence: 99%

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Bacillus clausii for Gastrointestinal Disorders: A Narrative Literature Review

Acosta-Rodríguez-Bueno

Abreu

Guarner

et al. 2022

Adv Ther

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The gut microbiota is intrinsically linked to human health; disturbances in microbial homeostasis are implicated in both intestinal and extraintestinal disorders. Probiotics are “live microorganisms that, when administered in adequate amounts, confer a health benefit on the host,” and many commercial preparations comprising a diverse range of species are available. While probiotics have been much researched, better understanding of the probiotic effects and applications of species such as Bacillus clausii is warranted. In this narrative literature review, we review the characteristics and mechanisms of action supporting B. clausii as a probiotic and discuss the evidence from clinical studies evaluating B. clausii probiotics for the management of a variety of gastrointestinal disorders and symptoms in children and adults. Finally, we highlight the challenges of future research and the need for more robust and diverse clinical evidence to guide physicians in the clinical application of probiotics for gastrointestinal disorders and other conditions. Supplementary Information The online version contains supplementary material available at 10.1007/s12325-022-02285-0.

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Section: Resultssupporting

confidence: 66%

Section: Resultsmentioning

confidence: 81%

Section: Resultsmentioning

confidence: 99%

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Bacillus clausii for Gastrointestinal Disorders: A Narrative Literature Review

Acosta-Rodríguez-Bueno

Abreu

Guarner

et al. 2022

Adv Ther

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“…Enolase is a protein that promotes the adherence of the strain to the gastro-intestinal tract of the host, while the fibronectin-binding protein allows the bacterium to adhere to fibronectin of the host. Additionally, the production of EPS by probiotic strains is linked with the adherence to intestinal mucus, while proteins with the LPXTG-type anchor, as the identified sortase, are associated both with cell surface localization and interaction with peptidoglycans [ 38 ]. Another desirable probiotic trait is the production of micronutrients, such as vitamins.…”

Section: Resultsmentioning

confidence: 99%

Draft Genome Sequence of Lactococcus lactis Subsp. cremoris WA2-67: A Promising Nisin-Producing Probiotic Strain Isolated from the Rearing Environment of a Spanish Rainbow Trout (Oncorhynchus mykiss, Walbaum) Farm

et al. 2022

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Probiotics are a viable alternative to traditional chemotherapy agents to control infectious diseases in aquaculture. In this regard, Lactococcus lactis subsp. cremoris WA2-67 has previously demonstrated several probiotic features, such as a strong antimicrobial activity against ichthyopathogens, survival in freshwater, resistance to fish bile and low pH, and hydrophobicity. The aim of this manuscript is an in silico analysis of the whole-genome sequence (WGS) of this strain to gain deeper insights into its probiotic properties and their genetic basis. Genomic DNA was purified, and libraries prepared for Illumina sequencing. After trimming and assembly, resulting contigs were subjected to bioinformatic analyses. The draft genome of L. cremoris WA2-67 consists of 30 contigs (2,573,139 bp), and a total number of 2493 coding DNA sequences (CDSs). Via in silico analysis, the bacteriocinogenic genetic clusters encoding the lantibiotic nisin Z (NisZ) and two new bacteriocins were identified, in addition to several probiotic traits, such as the production of vitamins, amino acids, adhesion/aggregation, and stress resistance factors, as well as the absence of transferable antibiotic resistance determinants and genes encoding detrimental enzymatic activities and virulence factors. These results unveil diverse beneficial properties that support the use of L. cremoris WA2-67 as a probiotic for aquaculture.

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“…All of these strains share a common ancestor, the KSM-K16 strain used in industrial applications [43]. The genome of B. clausii strain B106 reveals the presence of several genes that support its role as a probiotic: acid tolerance, bile tolerance, fibronectin-binding proteins, enolase, bacteriocins, synthesis of vitamins, and antibiotic resistance [44]. The genomes of the UBBC07 strain [45] and the AKU0647 strain [46] of B. clausii have also been sequenced.…”

Section: Preclinical Studies On the Probiotic Effects Of B Clausiimentioning

confidence: 99%

Current Progress and Future Perspectives on the Use of Bacillus clausii

et al. 2022

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Bacillus clausii is a probiotic that benefits human health. Its key characteristics include the ability to form spores; the resulting tolerance to heat, acid, and salt ensures safe passage through the human gastrointestinal tract with no loss of cells. Although B. clausii has been widely used for many decades, the beneficial properties of other probiotics, such as Lactobacillus spp. and Bifidobacterium spp., are better disseminated in the literature. In this review, we summarize the physiological, antimicrobial, and immunomodulatory properties of probiotic B. clausii strains. We also describe findings from studies that have investigated B. clausii probiotics from the perspective of quality and safety. We highlight innovative properties based on biochemical investigations of non-probiotic strains of B. clausii, revealing that B. clausii may have further health benefits in other therapeutic areas.

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Genome Profiling for Health Promoting and Disease Preventing Traits Unraveled Probiotic Potential of Bacillus clausii B106

Cited by 6 publications

References 54 publications

Bacillus clausii for Gastrointestinal Disorders: A Narrative Literature Review

Bacillus clausii for Gastrointestinal Disorders: A Narrative Literature Review

Draft Genome Sequence of Lactococcus lactis Subsp. cremoris WA2-67: A Promising Nisin-Producing Probiotic Strain Isolated from the Rearing Environment of a Spanish Rainbow Trout (Oncorhynchus mykiss, Walbaum) Farm

Current Progress and Future Perspectives on the Use of Bacillus clausii

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