Genome profiles in maternal blood during early onset preeclampsia and towards term

Dahlstrøm, Berit; Esbensen, Ying; Vollan, Hilde Synnøve; Øian, Pål; Bukholm, Geir

doi:10.1515/jpm.2010.095

Cited by 14 publications

(17 citation statements)

References 35 publications

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“…Matrix metalloproteinase ( MMP)-9 was found to be differentially expressed in that study, but was not confirmed in our qRT-PCR analysis. Dahlstrom et al, using the PAX gene Blood RNA system, reported 19 differentially expressed genes between 8 early-onset PE and 8 normal pregnant women [195]. Only lower expression of CCR3 (CC-chemokine receptor 3) gene in late-onset PE was confirmed in our study.…”

Section: Discussionsupporting

confidence: 66%

Differences and similarities in the transcriptional profile of peripheral whole blood in early and late-onset preeclampsia: insights into the molecular basis of the phenotype of preeclampsia^a

Chaiworapongsa¹,

Romero

Whitten³

et al. 2013

Journal of Perinatal Medicine

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Objective Preeclampsia (PE) has been sub-divided into early- and late-onset phenotypes. The pathogenesis of these two phenotypes has not been elucidated. To gain insight into the mechanisms of disease, the transcriptional profiles of whole blood from women with early- and late-onset PE were examined. Methods A cross-sectional study was conducted to include women with: 1) early-onset PE (diagnosed prior to 34 weeks, n=25); 2) late-onset PE (after 34 weeks, n=47); and 3) uncomplicated pregnancy (n=61). Microarray analysis of mRNA expression in peripheral whole blood was undertaken using Affymetrix microarrays. Differential gene expression was evaluated using a moderated t-test (false discovery rate <0.1 and fold change >1.5), adjusting for maternal WBC count and gestational age. Validation by real-time qRT-PCR was performed in a larger sample size [early PE (n=31), late PE (n=72) and controls (n=99)] in all differentially expressed genes. Gene Ontology analysis and pathway analysis were performed. Results 1) 43 and 28 genes were differentially expressed in early- and late-onset PE compared to the control group respectively; 2) qRT-PCR confirmed the microarray results for early and late-onset PE in 77% (33/43) and 71% (20/28) of genes, respectively; 3) 20 genes that are involved in coagulation (SERPINI2), immune regulation (VSIG4, CD24), developmental process (H19) and inflammation (S100A10) were differentially expressed in early-onset PE alone. In contrast, only seven genes that encoded proteins involved in innate immunity (LTF, ELANE) and cell-to-cell recognition in the nervous system (CNTNAP3) were differentially expressed in late-onset PE alone. Thirteen genes that encode proteins involved in host defense (DEFA4, BPI, CTSG, LCN2), tight junctions in blood-brain barrier (EMP1) and liver regeneration (ECT2) were differentially expressed in both early- and late-onset PE. Conclusion Early- and late-onset PE are characterized by a common signature in the transcriptional profile of whole blood. A small set of genes were differentially regulated in early- and late-onset PE. Future studies of the biological function, expression timetable and protein expression of these genes may provide insight into the pathophysiology of PE.

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Section: Discussionsupporting

confidence: 66%

Differences and similarities in the transcriptional profile of peripheral whole blood in early and late-onset preeclampsia: insights into the molecular basis of the phenotype of preeclampsia^a

Chaiworapongsa¹,

Romero

Whitten³

et al. 2013

Journal of Perinatal Medicine

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“…Maternal inflammatory reactions may change toward the end of the term. The study evaluating genome signaling in peripheral blood during preeclampsia and toward the end of the term using microarrays revealed upregulation of TNFSF15 (Dahlstrøm et al, 2010).…”

Section: Tnfsf15mentioning

confidence: 99%

Expression Profile of C19MC microRNAs in Placental Tissue in Pregnancy-Related Complications

Hromadníková

Kotlabova

Ondráčková

et al. 2015

DNA and Cell Biology

106

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“…Previous investigations have also shown the effect of vitamin D levels on peripheral blood gene expression during pregnancy and in preeclampsia (10,11). Global gene expression profiling has the potential to identify representative pathway genes that might point to the underlying molecular mechanism of preeclampsia (12)(13)(14)(15). Therefore, we performed a nested case-control study in the VDAART to identify vitamin D gene signatures related to preeclampsia during early pregnancy.…”

Section: Introductionmentioning

confidence: 99%

Early pregnancy vitamin D status and risk of preeclampsia

Mirzakhani

Litonjua

McElrath

et al. 2016

Journal of Clinical Investigation

180

154

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Genome profiles in maternal blood during early onset preeclampsia and towards term

Abstract: a possible type 1 immune response was identified both during preeclampsia and towards term. In pre-eclampsia a premature activation of leucocytes might be present.

Cited by 14 publications

References 35 publications

Differences and similarities in the transcriptional profile of peripheral whole blood in early and late-onset preeclampsia: insights into the molecular basis of the phenotype of preeclampsia^a

Differences and similarities in the transcriptional profile of peripheral whole blood in early and late-onset preeclampsia: insights into the molecular basis of the phenotype of preeclampsia^a

Expression Profile of C19MC microRNAs in Placental Tissue in Pregnancy-Related Complications

Early pregnancy vitamin D status and risk of preeclampsia

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Genome profiles in maternal blood during early onset preeclampsia and towards term

Abstract: a possible type 1 immune response was identified both during preeclampsia and towards term. In pre-eclampsia a premature activation of leucocytes might be present.

Cited by 14 publications

References 35 publications

Differences and similarities in the transcriptional profile of peripheral whole blood in early and late-onset preeclampsia: insights into the molecular basis of the phenotype of preeclampsiaa

Differences and similarities in the transcriptional profile of peripheral whole blood in early and late-onset preeclampsia: insights into the molecular basis of the phenotype of preeclampsiaa

Expression Profile of C19MC microRNAs in Placental Tissue in Pregnancy-Related Complications

Early pregnancy vitamin D status and risk of preeclampsia

Contact Info

Product

Resources

About

Differences and similarities in the transcriptional profile of peripheral whole blood in early and late-onset preeclampsia: insights into the molecular basis of the phenotype of preeclampsia^a

Differences and similarities in the transcriptional profile of peripheral whole blood in early and late-onset preeclampsia: insights into the molecular basis of the phenotype of preeclampsia^a