Genome plasticity of Vibrio parahaemolyticus: microevolution of the 'pandemic group'

Han, Haihong; Wong, Hin-Chung; Kan, Biao; Guo, Zhaobiao; Zeng, Xiao-Tao; Yin, Shaowu; Liu, Xiumei; Yang, Ruifu; Zhou, Dongsheng

doi:10.1186/1471-2164-9-570

Cited by 49 publications

(40 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S7). Such rearrangements are in agreement with the supposition that extensive genome plasticity is common in Vibrio species, particularly on C-II (40). Additionally, the V. antiquarius genome contains many perfect and approximate tandem repeats.…”

Section: Resultssupporting

confidence: 72%

Deep-sea hydrothermal vent bacteria related to human pathogenic Vibrio species

Hasan

Grim

Lipp

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Vibrio species are both ubiquitous and abundant in marine coastal waters, estuaries, ocean sediment, and aquaculture settings worldwide. We report here the isolation, characterization, and genome sequence of a novel Vibrio species, Vibrio antiquarius, isolated from a mesophilic bacterial community associated with hydrothermal vents located along the East Pacific Rise, near the southwest coast of Mexico. Genomic and phenotypic analysis revealed V. antiquarius is closely related to pathogenic Vibrio species, namely Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio harveyi, and Vibrio vulnificus, but sufficiently divergent to warrant a separate species status. The V. antiquarius genome encodes genes and operons with ecological functions relevant to the environment conditions of the deep sea and also harbors factors known to be involved in human disease caused by freshwater, coastal, and brackish water vibrios. The presence of virulence factors in this deep-sea Vibrio species suggests a far more fundamental role of these factors for their bacterial host. Comparative genomics revealed a variety of genomic events that may have provided an important driving force in V. antiquarius evolution, facilitating response to environmental conditions of the deep sea.W ith more than 110 recognized species, the genus Vibrio comprises a diverse group of heterotrophic bacteria, of which many are known pathogens, causing disease in animals and humans (1, 2). Vibrio cholerae is the most notorious because it is the causative agent of cholera. Vibrio vulnificus and Vibrio parahaemolyticus cause severe illness in humans and are associated with consumption of contaminated seafood (3, 4). Vibrio harveyi (5), Vibrio anguillarum (6, 7), and V. parahaemolyticus (8) continue to cause substantial economic losses to the aquaculture industry worldwide.Vibrios demonstrate a wide range of niche specialization: for example, free-living, attached to biotic and abiotic surfaces, and resident in both estuarine and marine habitats (9). The deep sea constitutes the largest habitat of the biosphere that supports microbial communities across three domains of life and represents an environment where physiochemical parameters-such as low temperature, high salinity, and high pressure-modulate community structure (10, 11). Several studies have shown the presence of physiologically, metabolically, and phylogenetically diverse mesophilic microbial communities in the deep sea, including Vibrio species (12-15). Barotolerant Vibrio spp. have been isolated from deep-sea sediment and from the gut microflora of invertebrates and fish collected from a variety of deepsea habitats, including hydrothermal vents (16,17). For example, strains of Vibrio, Aeromonas, and Pseudomonas spp. were isolated from mud-water samples collected at a depth of 4,940 m, 150 miles east of Cape Canaveral, Florida (18). Several culturedependent and -independent studies have confirmed the ubiquity of vibrios, and suggested Vibrio populations generally comprise approximately 1% (by molecular...

show abstract

Section: Resultssupporting

confidence: 72%

Deep-sea hydrothermal vent bacteria related to human pathogenic Vibrio species

Hasan

Grim

Lipp

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Molecular analysis including ribotyping, arbitrarily primed polymerase chain reaction (AP-PCR), and pulsed-field gel electrophoresis (PFGE) revealed that O3:K6 and its serovariants exhibit a high degree of genetic similarity among each other but differ from O3:K6 strains isolated before 1996 [16][17][18]. Notably, pandemic O3:K6 isolates were shown to be generally positive for the thermostable direct hemolysin (TDH) but negative for the TDH-related hemolysin (TRH; see the section entitled "TDH and TRH toxins," later in this chapter) [19].…”

Section: Parahaemolyticusmentioning

confidence: 99%

Vibrio parahaemolyticus virulence determinants

Santos

Salomon

Krachler

et al. 2015

The Comprehensive Sourcebook of Bacterial Protein Toxins

View full text Add to dashboard Cite

“…To date, the so-called 'pandemic group' includes 21 serotypes, based on nearly identical genotypes and molecular profiles, referred to as serovariants of O3:K6 isolates (Nair et al, 2007). Currently, there is no unanimity in the scientific community with regard to identifying a single marker for pandemic strains and suspected V. parahaemolyticus pandemic isolates have been identified by positive group-specific PCR, based on a sequence variation in the toxRS gene (Okura et al, 2003;Han et al, 2008) and the presence of the tdh gene. Quilici et al (2005) and Martinez-Urtaza et al (2005) reported evidence that pandemic V. parahaemolyticus had migrated into Europe and has been isolated from clinical cases in France (Quilici et al, 2005), in Spain (Martinez-Urtaza et al, 2005) and in Russia during gastroenteritis outbreaks (Smolikova et al, 2001).…”

mentioning

confidence: 99%

Serological and molecular characterization of Vibrio parahaemolyticus marine strains carrying pandemic genetic markers

Caburlotto¹,

Gennari²,

Ghidini³

et al. 2010

The ISME Journal

View full text Add to dashboard Cite

In 2005, pandemic Vibrio parahaemolyticus was reported to have been introduced in Europe: O3:K6 strains were isolated from clinical cases in France and Spain, and were found to be associated with consumption of contaminated seafood. On the contrary, pandemic strains were not isolated from seafood or from the environment itself. Analysis of two V. parahaemolyticus strains isolated in May 2007 from Northern Italy seawater and plankton samples revealed the presence of the virulence gene tdh and the pandemic-specific markers orf8 and toxRS/new sequence (group-specific PCR). The two strains showed serotypes not included in the 'pandemic group', but their molecular typing proved that they represent a single clone showing a genetic profile very similar to that of pandemic O3:K6 reference isolates. Moreover, the two marine strains carried three virulence-related genes associated with clinical strains and, to date, hardly ever or never detected in environmental strains. The presence, in strains isolated from the marine environment, of genetic pandemic markers and virulence genes normally associated with clinical isolates proves that marine strains might constitute a public health concern.

show abstract

Genome plasticity of Vibrio parahaemolyticus: microevolution of the 'pandemic group'

Cited by 49 publications

References 29 publications

Deep-sea hydrothermal vent bacteria related to human pathogenic Vibrio species

Deep-sea hydrothermal vent bacteria related to human pathogenic Vibrio species

Vibrio parahaemolyticus virulence determinants

Serological and molecular characterization of Vibrio parahaemolyticus marine strains carrying pandemic genetic markers

Contact Info

Product

Resources

About