Genome Organization of Four Brazilian Xanthomonas albilineans Strains Does Not Correlate with Aggressiveness

Miranda, Raquel P; Turrini, Paula Cristina Gasperazzo; Bonadio, Dora T; Zerillo, Marcelo M.; Berselli, Arthur P; Creste, Silvana; Sluys, Marie‐Anne Van

doi:10.1128/spectrum.02802-22

Cited by 2 publications

(4 citation statements)

References 50 publications

(83 reference statements)

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“…Ecological conditions such as soil, climate, water, and other factors differ by geographical locations where sugarcane grows and these conditions can also promote genetic differentiation of pathogen populations [ 13 ]. Genetic diversity revealed by a phylogenomic tree based on 21 public genomic sequences of worldwide Xa strains can be grouped based on their geographic origin (American, African, or Asiatic), to some degree [ 28 ]. Recombination-driven horizontal gene transfer is an important factor that contributes to pathogen population structure and diversity across different Xanthomonas spp.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to the MLSA and SSR methods, other fingerprinting tools have also been used to investigate Xa genetic divergence, including restriction fragment length polymorphism (RFLP) [ 18 ], random amplified fragment polymorphism (RAPD) [ 23 ], rep-PCR [ 24 ], and amplified fragment length polymorphism (AFLP) [ 25 ]. Recently, whole-genome sequencing of Xa strains was performed on two strains from China [ 26 , 27 ] and four strains from Brazil [ 28 ]. These strains were further used together alongside publicly available strains in the NCBI database to explore genomic diversity.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Divergence and Population Structure of Xanthomonas albilineans Strains Infecting Saccharum spp. Hybrid and Saccharum officinarum

Ntambo

Zhao

et al. 2023

Plants

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Leaf scald caused by Xanthomonas albilineans (Xa) is a major bacterial disease in sugarcane that represents a threat to the global sugar industry. Little is known about the population structure and genetic evolution of this pathogen. In this study, 39 Xa strains were collected from 6 provinces in China. Of these strains, 15 and 24 were isolated from Saccharum spp. hybrid and S. officinarum plants, respectively. Based on multilocus sequence analysis (MLSA), with five housekeeping genes, these strains were clustered into two distinct phylogenetic groups (I and II). Group I included 26 strains from 2 host plants, Saccharum spp. hybrid and S. officinarum collected from 6 provinces, while Group II consisted of 13 strains from S. officinarum plants in the Zhejiang province. Among the 39 Xa strains, nucleotide sequence identities from 5 housekeeping genes were: ABC (99.6–100%), gyrB (99.3–100%), rpoD (98.4–100%), atpD (97.0–100%), and glnA (97.6–100%). These strains were clustered into six groups (A–F), based on the rep-PCR fingerprinting, using primers for ERIC2, BOX A1R, and (GTG)5. UPGMA and PCoA analyses revealed that group A had the most strains (24), followed by group C with 11 strains, while there was 1 strain each in groups B and D–F. Neutral tests showed that the Xa population in S. officinarum had a trend toward population expansion. Selection pressure analysis showed purification selection on five concatenated housekeeping genes from all tested strains. Significant genetic differentiation and infrequent gene flow were found between two Xa populations hosted in Saccharum spp. hybrids and S. officinarum. Altogether, these results provide evidence of obvious genetic divergence and population structures among Xa strains from China.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic Divergence and Population Structure of Xanthomonas albilineans Strains Infecting Saccharum spp. Hybrid and Saccharum officinarum

Ntambo

Zhao

et al. 2023

Plants

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“…Xanthomonas albilineans isolate Xa11 (Xa11) was obtained from a symptomatic plant in a sugarcane-growing region in Brazil, confirmed as X. albilineans by conventional PCR and selected for the present work given its high aggressiveness to sugarcane SP78-4467-susceptible genotype ( Tardiani et al 2014 ). The isolate Xa11 was grouped with an average nucleotide identity of 99.94% after genome sequencing with the Guadeloupe Island Strain GPE PC73 ( Miranda et al 2023 ), which belongs to a specific genetic subgroup known as PFGE-B ( Pieretti et al 2012 ) and serotype 1 ( Pieretti et al 2015a ). Xa11 is phylogenetic related to American strains clustering of X. albilineans ( Miranda et al 2023 ).…”

Section: Methodsmentioning

confidence: 99%

“…The isolate Xa11 was grouped with an average nucleotide identity of 99.94% after genome sequencing with the Guadeloupe Island Strain GPE PC73 ( Miranda et al 2023 ), which belongs to a specific genetic subgroup known as PFGE-B ( Pieretti et al 2012 ) and serotype 1 ( Pieretti et al 2015a ). Xa11 is phylogenetic related to American strains clustering of X. albilineans ( Miranda et al 2023 ). Xa11 was stored at −80 °C in 20% glycerol (v:v), for long-term maintenance at the microorganism’s collection of “Instituto Agronômico (IAC), Centro de Cana, Ribeirão Preto-SP, Brazil (21º11ʹ S, 47º48ʹ W).”…”

Section: Methodsmentioning

confidence: 99%

Transmission of Xanthomonas albilineans by the spittlebug, Mahanarva fimbriolata (Hemiptera: Cercopidae), in Brazil: first report of an insect vector for the causal agent of sugarcane leaf scald

Bini,

Serra,

Pastore

et al. 2023

Journal of Insect Science

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Leaf scald is a destructive sugarcane disease caused by the bacterium Xanthomonas albilineans (Ashby) Dowson. This pathogen presents the gene cluster SPI-1 T3SS, a conserved feature in pathogens vectored by animals. In this study, the competence of Mahanarva fimbriolata (Stål), a spittlebug commonly found in sugarcane fields in Brazil, was evaluated for the transmission of X. albilineans. Artificial probing assays were conducted to investigate the ability of M. fimbriolata adults to acquire X. albilineans from artificial diets containing the pathogen with subsequent inoculation of X. albilineans into pathogen-free diets. Plant probing assays with M. fimbriolata adults were conducted to evaluate the acquisition of X. albilineans from diseased source plants and subsequent inoculation of healthy recipient sugarcane plants. The presence of X. albilineans DNA in saliva/diet mixtures of the artificial probing assays and both insects and plants of the plant probing assays were checked using TaqMan assays. The artificial probing assays showed that M. fimbriolata adults were able to acquire and inoculate X. albilineans in diets. Plant probing assays confirmed the competence of M. fimbriolata to transmit X. albilineans to sugarcane. Over the entire experiment, 42% of the insects had acquired the pathogen and successful inoculation of the pathogen occurred in 18% of the recipient-susceptible sugarcane plants at 72 or 96 h of inoculation access period. Assays evidenced the vector competence of M. fimbriolata for transmission of X. albilineans, opening new pathways for investigating the biology and the economic impacts of the interaction between X. albilineans and M. fimbriolata.

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Genome Organization of Four Brazilian Xanthomonas albilineans Strains Does Not Correlate with Aggressiveness

Cited by 2 publications

References 50 publications

Genetic Divergence and Population Structure of Xanthomonas albilineans Strains Infecting Saccharum spp. Hybrid and Saccharum officinarum

Genetic Divergence and Population Structure of Xanthomonas albilineans Strains Infecting Saccharum spp. Hybrid and Saccharum officinarum

Transmission of Xanthomonas albilineans by the spittlebug, Mahanarva fimbriolata (Hemiptera: Cercopidae), in Brazil: first report of an insect vector for the causal agent of sugarcane leaf scald

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