Genome organisation and comparative genomics of four novel Wolbachia genome assemblies from Indian Drosophila host

Singhal, Kopal; Mohanty, Sujata

doi:10.1007/s10142-019-00664-5

Cited by 4 publications

(1 citation statement)

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“…Interestingly, these genes involved in host cell attachment have been described as lost in Rickettsiales genomes as well as wMel and wRi (Pichon, Bouchon et al 2009) [43]. More recently, it has been highlighted that some Wolbachia genomes contain VirB2, such as some strains infecting Drosophila species [44], those infecting Dactylopius species [45] and Wolbachia from Aedes albopictus [46]. Our results indicate presence of VirB2 in all Wolbachia analyzed, with the exception of wPpe, and only one copy is observed in the genomes of wOo, wOv and wBm (Additional file 9, Table S5).…”

Section: Metabolic Pathwaysmentioning

confidence: 99%

Pseudoscorpion Wolbachia symbionts: Diversity and Evidence for a New Supergroup S

Lefoulon

Clark

Borvető

et al. 2020

Preprint

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Abstract Background Wolbachia are the most widely spread endosymbiotic bacteria, present in a wide variety of insects and two families of nematodes, but so far, relatively little genomic data is available. The Wolbachia symbiont can be a parasite, as described for many arthropods, or an obligate mutualist, as in filarial nematodes. Although, the nature of these symbioses remains largely unknown, diverse Wolbachia genomic data will contribute to understanding their diverse symbiotic mechanisms. Results Our study focuses on Wolbachia infections in pseudoscorpion species collected in Montpellier (France) and indicates two distinct groups of Wolbachia : Geogarypus minor harbors Wolbachia ( w Gmin) and Chthonius ischnocheles harbors Wolbachia ( w Cisc), both related to supergroup H and Atemnus politus harbours Wolbachia ( w Apol), forming a novel divergent clade with Wolbachia from the pseudoscorpion Cordylochernes scorpioides . This later clade forms a new supergroup S, most closely related to Wolbachia supergroups C and F. Our data suggest multiple symbiont acquisition events within the evolutionary history of pseudoscorpions. Using target enrichment by hybridization with Wolbachia -specific biotinylated probes to capture large fragments of Wolbachia DNA, we produced a draft genome of w Apol characterized by a moderate Wolbachia size (1,445,964bp) containing a moderate number of transposable elements and WO bacteriophage insertions (total of 77,522 bp). Conclusions Our analyses indicate that w Apol forms a diverget clade. Annotation highlights complete biochemical pathways which are incomplete in many sequenced Wolbachia genomes, such as vitamin B and the cytochrome bd ubiquinol oxidase pathway. Further, the biotin operon appears to have been horizontally transferred multiple times along the Wolbachia evolutionary history.