Genome Engineering with TALE and CRISPR Systems in Neuroscience

Lee, Han B.; Sundberg, Brynn N.; Sigafoos, Ashley N.; Clark, Karl J.

doi:10.3389/fgene.2016.00047

Cited by 27 publications

(22 citation statements)

References 280 publications

(347 reference statements)

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“…Thus, the hypothesis of a shared genetic factor underlying the expression of female courtship in both females and sneakers is the most parsimonious of the two presented above. Newly available techniques of genome editing (e.g., CRISPR/Cas9, Heidenreich & Zhang, ; Lee, Sundberg, Sigafoos, & Clark, ) open the door to testing the role of specific candidate genes in the control of female courtship behaviour in both females and sneakers.…”

Section: Discussionmentioning

confidence: 99%

Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish

et al. 2017

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Distinct patterns of gene expression often underlie intra- and intersexual differences, and the study of this set of coregulated genes is essential to understand the emergence of complex behavioural phenotypes. Here, we describe the development of a de novo transcriptome and brain gene expression profiles of wild-caught peacock blenny, Salaria pavo, an intertidal fish with sex-role reversal in courtship behaviour (i.e., females are the courting sex) and sequential alternative reproductive tactics in males (i.e., larger and older nest-holder males and smaller and younger sneaker males occur). Sneakers mimic both female's courtship behaviour and nuptial coloration to get access to nests and sneak fertilizations, and later in life transition into nest-holder males. Thus, this species offers the unique opportunity to study how the regulation of gene expression can contribute to intersex phenotypes and to the sequential expression of male and female behavioural phenotypes by the same individual. We found that at the whole brain level, expression of the sneaker tactic was paralleled by broader and divergent gene expression when compared to either females or nest-holder males, which were more similar between themselves. When looking at sex-biased transcripts, sneaker males are intersex rather than being either nest-holder or female-like, and their transcriptome is simultaneously demasculinized for nest-holder-biased transcripts and feminized for female-biased transcripts. These results indicate that evolutionary changes in reproductive plasticity can be achieved through regulation of gene expression, and in particular by varying the magnitude of expression of sex-biased genes, throughout the lifetime of the same individual.

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Section: Discussionmentioning

confidence: 99%

Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish

et al. 2017

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“…In addition, although not considered a gene targeting method per se , optogenetics (which allow light controlled activation or deactivation of particular neuronal circuits, Gross (2011) ) and promising combinations between pharmacology and genetics, such as DREADDS (which utilizes genetically modified channels or receptors designed to bind particular artificial ligands, Whissell et al (2016) ), may also enrich the toolset of the neurobiologist leading to unprecedented sophistication with which the central nervous system may be probed. Some of these techniques are discussed in this very issue/special topic ( Lee et al, 2016 ; Walters et al, 2016 ; Whissell et al, 2016 ), others have been reviewed and explained in detail elsewhere. Thus, I only briefly discuss the novel gene targeting systems, particularly in the context of how they compare to classical gene targeting.…”

Section: Will Homologous Recombination-based Gene Targeting Survive Tmentioning

confidence: 99%

“…It can be conducted in any species. For example, while classical gene targeting is currently restricted to the house mouse and recently extended to the rat, the TALEN-based gene targeting method is not dependent upon the availability of ES cells, and has already been successfully utilized in a range of species, including, for example, one of the simplest vertebrates, the zebrafish ( Clark et al, 2011 ; Lee et al, 2016 ). The method utilizes the transcription activator-like effector proteins or TALEs, which are site-specific DNA-binding proteins discovered in Xanthomonas species, bacterial pathogens of plants.…”

Section: Alternatives Of Homologous Recombination In Es Cell-based Gementioning

confidence: 99%

Gene Targeting Using Homologous Recombination in Embryonic Stem Cells: The Future for Behavior Genetics?

Gerlai

2016

Front. Genet.

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Gene targeting with homologous recombination in embryonic stem cells created a revolution in the analysis of the function of genes in behavioral brain research. The technology allowed unprecedented precision with which one could manipulate genes and study the effect of this manipulation on the central nervous system. With gene targeting, the uncertainty inherent in psychopharmacology regarding whether a particular compound would act only through a specific target was removed. Thus, gene targeting became highly popular. However, with this popularity came the realization that like other methods, gene targeting also suffered from some technical and principal problems. For example, two decades ago, issues about compensatory changes and about genetic linkage were raised. Since then, the technology developed, and its utility has been better delineated. This review will discuss the pros and cons of the technique along with these advancements from the perspective of the neuroscientist user. It will also compare and contrast methods that may represent novel alternatives to the homologous recombination based gene targeting approach, including the TALEN and the CRISPR/Cas9 systems. The goal of the review is not to provide detailed recipes, but to attempt to present a short summary of these approaches a behavioral geneticist or neuroscientist may consider for the analysis of brain function and behavior.

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“…Synthetic biology covers various aspects, including genetic circuits, minimal genomes, DNA synthesis, and genetic modification [ 2 ]. And also, synthetic biology can be developed in organisms with reverse genetic tools and methodologies, including genetic transformation, selection markers, specific plasmid vectors, different promoters, and other advanced molecular biology techniques, such as (zinc finger proteins) ZFPs, (transcription activators like effector nucleases) TALENs, and (clustered regularly interspaced short palindromic repeats) CRISPR [ 3 , 4 ]. To provide insight to further understand synthetic biology and discuss future perspectives in this area, the most updated techniques occurring in synthetic biology are summarized in this review.…”

Section: Introductionmentioning

confidence: 99%

Genetic tool development and systemic regulation in biosynthetic technology

Dai

Zhang

Qiao

et al. 2018

Biotechnol Biofuels

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With the increased development in research, innovation, and policy interest in recent years, biosynthetic technology has developed rapidly, which combines engineering, electronics, computer science, mathematics, and other disciplines based on classical genetic engineering and metabolic engineering. It gives a wider perspective and a deeper level to perceive the nature of life via cell mechanism, regulatory networks, or biological evolution. Currently, synthetic biology has made great breakthrough in energy, chemical industry, and medicine industries, particularly in the programmable genetic control at multiple levels of regulation to perform designed goals. In this review, the most advanced and comprehensive developments achieved in biosynthetic technology were represented, including genetic engineering as well as synthetic genomics. In addition, the superiority together with the limitations of the current genome-editing tools were summarized.

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Genome Engineering with TALE and CRISPR Systems in Neuroscience

Cited by 27 publications

References 280 publications

Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish

Temporal variation in brain transcriptome is associated with the expression of female mimicry as a sequential male alternative reproductive tactic in fish

Gene Targeting Using Homologous Recombination in Embryonic Stem Cells: The Future for Behavior Genetics?

Genetic tool development and systemic regulation in biosynthetic technology

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