Genetic tool development and systemic regulation in biosynthetic technology

Abstract

With the increased development in research, innovation, and policy interest in recent years, biosynthetic technology has developed rapidly, which combines engineering, electronics, computer science, mathematics, and other disciplines based on classical genetic engineering and metabolic engineering. It gives a wider perspective and a deeper level to perceive the nature of life via cell mechanism, regulatory networks, or biological evolution. Currently, synthetic biology has made great breakthrough in energy, chemical industry, and medicine industries, particularly in the programmable genetic control at multiple levels of regulation to perform designed goals. In this review, the most advanced and comprehensive developments achieved in biosynthetic technology were represented, including genetic engineering as well as synthetic genomics. In addition, the superiority together with the limitations of the current genome-editing tools were summarized.

Recent Advances of CRISPR/Cas9-Based Genetic Engineering and Transcriptional Regulation in Industrial Biology

“…The core concept of synthetic biology is to transform existing natural systems and construct gene circuits by building and integrating standardized components and modules (Dai et al, 2018). However, an ultimate challenge in the construction of gene circuits was lack of effective, programmable, secure, and sequence-specific gene editing tools.…”

Aspergillus fumigatus AR04 obeys Arrhenius' rule in cultivation temperature shifts from 30 to 40°C

“…Currently, artificial genome reduction and chromosome synthesis are performed to gain both, an understanding of a system reduced in complexity as well as a cell factory equipped with a minimum of structure and an optimum of function (Dai et␣al , 2018). There are no reports published that growth rate increased for Bacillus subtilis after genome reduction.…”

Microbial Biodiversity and Bioremediation Assessment Through Omics Approaches

“…In genome driven approach reporter gene-based in vivo screening and selection strategies are employed. The ease in accessing metagenomics sequencing data and the availability of multiple softwares makes this approach in demand for exploring metagenomic libraries (Lin et al, 2006;Dai et al, 2018). Genome driven approach is dependent on reporter gene present in the clones which may be colorimetric (LacZ), fluorescent (GFP), bioluminescent (LuxCDABE), conditional survival (CAT), acidification (AraBAD), ice nucleation (InaZ), motility (CheZ), cell display (LamB), etc.…”