Genome engineering for crop improvement and future agriculture

Gao, Caixia

doi:10.1016/j.cell.2021.01.005

Cited by 517 publications

(397 citation statements)

References 145 publications

(177 reference statements)

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“…Protein & Cell LETTER Additionally, retrons coupled with CRISPR can efficiently insert a GFP gene in yeast with efficiency up to 87% (Sharon et al, 2018), making us anticipating retron editing a more versatile genome editor in both therapeutic applications and crop improvement. Precise genome editing on locus corresponding to agronomic traits can greatly accelerate the crop breeding (Mao et al, 2019;Gao, 2021). Although, base transversions, small insertion and deletion by base editors and prime editors have been successfully achieved in plant cells (Gao, 2021).…”

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confidence: 99%

Precise genome editing without exogenous donor DNA via retron editing system in human cells

et al. 2021

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Precise genome editing without exogenous donor DNA via retron editing system in human cells

et al. 2021

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“…Meanwhile, NP-mediated passive delivery has been reported with tobacco (Burlaka et al, 2015;Mitter et al, 2017;Golestanipour et al, 2018;Kwak et al, 2019), cowpea (Mitter et al, 2017), and arugula crops (Kwak et al, 2019). NPs and other new materials might serve as useful vehicles for editing systems (Gao, 2021). Working within this context, Hamada et al (2017) proposed a method involving plant bombardment in which the shoot apical meristems of wheat were used as the target tissue (Imai et al, 2020).…”

Section: Advanced Plant Biomolecule Delivery Approaches Via the Application Of Nanobiotechnologymentioning

confidence: 99%

Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

et al. 2021

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Agriculture is an important source of human food. However, current agricultural practices need modernizing and strengthening to fulfill the increasing food requirements of the growing worldwide population. Genome editing (GE) technology has been used to produce plants with improved yields and nutritional value as well as with higher resilience to herbicides, insects, and diseases. Several GE tools have been developed recently, including clustered regularly interspaced short palindromic repeats (CRISPR) with nucleases, a customizable and successful method. The main steps of the GE process involve introducing transgenes or CRISPR into plants via specific gene delivery systems. However, GE tools have certain limitations, including time-consuming and complicated protocols, potential tissue damage, DNA incorporation in the host genome, and low transformation efficiency. To overcome these issues, nanotechnology has emerged as a groundbreaking and modern technique. Nanoparticle-mediated gene delivery is superior to conventional biomolecular approaches because it enhances the transformation efficiency for both temporal (transient) and permanent (stable) genetic modifications in various plant species. However, with the discoveries of various advanced technologies, certain challenges in developing a short-term breeding strategy in plants remain. Thus, in this review, nanobased delivery systems and plant genetic engineering challenges are discussed in detail. Moreover, we have suggested an effective method to hasten crop improvement programs by combining current technologies, such as speed breeding and CRISPR/Cas, with nanotechnology. The overall aim of this review is to provide a detailed overview of nanotechnology-based CRISPR techniques for plant transformation and suggest applications for possible crop enhancement.

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“…This is useful not only for basic research such as functional genomics but also for crop breeding. The current state‐of‐play in crop breeding by genome editing is summarized in recent reviews (Gao, 2021; Zhu et al, 2020). The merit of crop breeding by genome editing is that an elite variety can be modified directly and rapidly.…”

Section: Crop Breedingmentioning

confidence: 99%

Plant genome editing: ever more precise and wide reaching

2021

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SUMMARY Genome‐editing technologies consisting of targeted mutagenesis and gene targeting enable us to modify genes of interest rapidly and precisely. The discovery in 2012 of CRISPR/Cas9 systems and their development as sequence‐specific nucleases has brought about a paradigm shift in biology. Initially, CRISPR/Cas9 was applied in targeted mutagenesis to knock out a target gene. Thereafter, advances in genome‐editing technologies using CRISPR/Cas9 developed rapidly, with base editing systems for transition substitution using a combination of Cas9 nickase and either cytidine or adenosine deaminase being reported in 2016 and 2017, respectively, and later in 2021 bringing reports of transversion substitution using Cas9 nickase, cytidine deaminase and uracil DNA glycosylase. Moreover, technologies for gene targeting and prime editing systems using DNA or RNA as donors have also been developed in recent years. Besides these precise genome‐editing strategies, reports of successful chromosome engineering using CRISPR/Cas9 have been published recently. The application of genome editing to crop breeding has advanced in parallel with the development of these technologies. Genome‐editing enzymes can be introduced into plant cells, and there are now many examples of crop breeding using genome‐editing technologies. At present, it is no exaggeration to say that we are now in a position to be able to modify a gene precisely and rearrange genomes and chromosomes in a predicted way. In this review, we introduce and discuss recent highlights in the field of precise gene editing, chromosome engineering and genome engineering technology in plants.

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Genome engineering for crop improvement and future agriculture

Cited by 517 publications

References 145 publications

Precise genome editing without exogenous donor DNA via retron editing system in human cells

Precise genome editing without exogenous donor DNA via retron editing system in human cells

Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

Plant genome editing: ever more precise and wide reaching

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