Genome-editing technologies and their potential application in horticultural crop breeding

Xiong, Jinsong; Ding, Jing; Li, Yang

doi:10.1038/hortres.2015.19

Cited by 131 publications

(70 citation statements)

References 96 publications

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“…As one of the earliest agricultural activities, plant breeding has begun in very ancient times (early on Neolithic revolution, 10,000 BC) parallel to human culture [1,2]. For the plant breeding, genetic variations are the pre-request.…”

Section: Introductionmentioning

confidence: 99%

The Use of Gamma Irradiation in Plant Mutation Breeding

Beyaz¹,

Yıldız²

2017

Plant Engineering

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In plant breeding programs, one of the oldest methods is mutation breeding. Currently, mutation breeding has became popular among the breeders and scientists again with its use in plant biotechnology and due to some restrictions on the other techniques such as hybridization, cross breeding, and transgenic plants. Physical mutagens (X-rays, UV light, neutrons-alpha-beta particles, fast and thermal neutrons, especially gamma rays) are used more widely than chemical (ethyl methanesulfonate [EMS]) ones to artificially induce mutations (mutagenesis). However, among the physical mutagens, gamma-rays are widely used. During the irradiation of the seeds with ionizing radiation to generate mutants with desirable traits, reactive oxygen species (ROS) or free radicals can generate in cells. Although, these radicals/species generally can be very dangerous for the cell compartments, they can take an important role as a signal molecule activation of genes of antioxidant enzymes and proline, which are defense systems against these radicals in plant cells. In this chapter, usability of gamma-irradiation to provide the permanent gene expression of antioxidant enzymes and proline through the production of reactive oxygen species (ROS) is discussed.

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Section: Introductionmentioning

confidence: 99%

The Use of Gamma Irradiation in Plant Mutation Breeding

Beyaz¹,

Yıldız²

2017

Plant Engineering

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“…This results in a reduction of land, water, pesticide, and fertilizer use [82]. In addition, genome editing for inhibition of ethylene biosynthesis, which plays a very important role in fruit ripening process [82] or its signaling pathways, enables creation of new varieties with extended shelf life [63].…”

Section: Application Of Genome Editing Systems Inmentioning

confidence: 99%

“…It is also possible to overcome this 5 Thymidine constraint by developing mutant variants of TALEN Nterminal domain which can bind other nucleotides [57]. Considering the ease of site-directed manipulation using TALEN system, within a short period of time after the unraveling of the TALEN mode of action, the genes modified by this system have been used successfully in several animal and plant species and the plant examples include rice, wheat, Arabidopsis, potato, and tomato (Table 2) [63].…”

Section: Transcription Activator-like Effector Nucleases (Talens)mentioning

confidence: 99%

“…Genome editing technologies have wide practical applications for solving one of the most important tasks of modern biotechnology-the creation of new varieties of crops, which are high-yielding and resistant to abiotic and biotic stresses and also have high nutritional value (Table 2) [31,63,[75][76][77][78][79][80]. To this end, genome editing system has been used in plant breeding (1) to insert point mutations similar to natural SNPs [26,27], (2) to make small modifications to gene function [13], (3) for integration of foreign genes, (4) for gene pyramiding and knockout, and (5) for the repression or activation of gene expression, as well as (6) epigenetic editing [6].…”

Section: Application Of Genome Editing Systems Inmentioning

confidence: 99%

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Genome Editing in Plants: An Overview of Tools and Applications

Kamburova

Никитина

Shermatov

et al. 2017

International Journal of Agronomy

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The emergence of genome manipulation methods promises a real revolution in biotechnology and genetic engineering. Targeted editing of the genomes of living organisms not only permits investigations into the understanding of the fundamental basis of biological systems but also allows addressing a wide range of goals towards improving productivity and quality of crops. This includes the creation of plants with valuable compositional properties and with traits that confer resistance to various biotic and abiotic stresses. During the past few years, several novel genome editing systems have been developed; these include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9). These exciting new methods, briefly reviewed herein, have proved themselves as effective and reliable tools for the genetic improvement of plants.

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“…One such novel breeding technology is targeted genome editing (TGE) using engineered nucleases (EN). TGE has emerged as an alternative breeding tool for developing plants with novel traits (Xiong et al, 2016). TGE mainly relies on four types of ENs, including homing endonucleases or meganucleases (EMNs), zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspersed short palindromic repeats (CRISPR)/Cas9 reagents (Gaj et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Targeted genome editing, an alternative tool for trait improvement in horticultural crops

Subburaj

Jin

et al. 2016

Hortic. Environ. Biotechnol.

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Abstract. Improving crops through plant breeding, an important approach for sustainable agriculture, has been utilized to increase the yield and quality of foods and other biomaterials for human use. Crops, including cereals, vegetables, ornamental flowers, fruits, and trees, have long been cultivated to produce high-quality products for human consumption. Conventional breeding technologies, such as natural cross-hybridization, mutation induction through physical or chemical mutagenesis, and modern transgenic tools are often used to enhance crop production. However, these breeding methods are sometimes laborious and complicated, especially when attempting to improve desired traits without inducing pleiotropic effects. Recently, targeted genome editing (TGE) technology using engineered nucleases, including meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR) nucleases, has been used to improve the traits of economically important plants. TGE has emerged as a novel plant-breeding tool that represents an alternative approach to classical breeding, but with higher mutagenic efficiency. Here, we briefly describe the basic principles of TGE and the types of engineered nucleases utilized, along with their advantages and disadvantages. We also discuss their potential use to improve the traits of horticultural crops through genome engineering.

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Genome-editing technologies and their potential application in horticultural crop breeding

Cited by 131 publications

References 96 publications

The Use of Gamma Irradiation in Plant Mutation Breeding

The Use of Gamma Irradiation in Plant Mutation Breeding

Genome Editing in Plants: An Overview of Tools and Applications

Targeted genome editing, an alternative tool for trait improvement in horticultural crops

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