Genome editing and its applications in genetic improvement in aquaculture

Yang, Zituo; Yu, Yang; Tay, Yi Xuan; Yue, Gen Hua

doi:10.1111/raq.12591

Cited by 62 publications

(49 citation statements)

References 134 publications

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“…To induce heritable modifications through gene editing, it must happen very early in the development, ideally at the onecell stage so that they can be incorporated in its germ line, hence the need for specialized methods like microinjection to deliver the CRISPR/Cas complex on a microscopic scale. Nevertheless, other procedures to deliver the CRISPR/Cas9 complex into one-cell fertilized eggs or cultured cell are lentivirus-mediated methods, electroporation, and ribonucleoprotein complexes (Okoli et al, 2021;Yang et al, 2022). Mutants should be carefully screened using sequence analysis, and off-target affects should be assessed.…”

Section: Advances Of Crispr/cas-genome Editing In Aquaculture and Fis...mentioning

confidence: 99%

“…-For the rapid selection of optimal gRNAs, CRISPR/Cas constructs better to be tested in the cell culture before in vivo editing (Gratacap et al, 2020;Yang et al, 2022). However, a problem arises since for aquaculture research, there is a short fall of well-characterized and suitable cell lines for many species of interest.…”

Section: Technical Challengesmentioning

confidence: 99%

“…-CRISPR/Cas construct needs to be delivered into fertilized eggs at the one-cell stage in fish (Güralp et al, 2020;Yang et al, 2022). The timing for the development of the fertilized egg to first cell division varies from species to species in fish; therefore, it is essential to know the time duration from fertilization to the first cell division so that the critical time for genome editing is not missed.…”

Section: Technical Challengesmentioning

confidence: 99%

“…In GMOs, new-foreign genes combine into the original DNA, whereas CRISPR genome editing does not introduce any foreign DNA into genome of interest species. Rather, it is a small-controlled change to the original DNA that improves the targeted trait by removing the inferior alleles (Yang et al, 2022). This means that the GMOs product/fish is not a creature that would exist in nature, whereas CRISPR-edited product/fish where the original genes are partly altered similar to the selective breeding process, thus shortening the mutation evolution process that occurs in nature over many generations of breeding.…”

Section: Regulatory and Consumer Acceptancementioning

confidence: 99%

See 3 more Smart Citations

CRISPR/Cas Genome Editing—Can It Become a Game Changer in Future Fisheries Sector?

Roy

Kumar²,

Behera³

et al. 2022

Front. Mar. Sci.

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Fisheries and aquaculture are the fastest-growing food-producing sector and rapidly becoming an important element for the global food security since they are the primary source of seafood and high animal protein in the human diet. Genome editing offers new possibilities such as the clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology, which has the potential to accelerate the sustainable genetic improvement in fisheries and aquaculture. The CRISPR/Cas9 system has four key components, namely, target DNA, Cas9, the protospacer adjacent motif sequence, and the guide RNA or single-guide RNA. CRISPR/Cas is cheaper, easier, and more precise than the other genome editing technologies and can be used as a new breeding technology in fisheries and aquaculture to solve the far-reaching challenges. The attributes like high fecundity, external fertilization, short generation interval, the established method of breeding, and the larval rearing of most aquaculture species have advantages for CRISPR/Cas9 genome editing applications. CRISPR/Cas9 has recently been applied to the traits valued in some aquaculture species (almost >20 species), targeting the main traits of traditional genetic improvement initiatives like growth, disease resistance, reproduction, sterility, and pigmentation. Genome editing can fast forward the breeding process with precision where changes occur in the targeted genes. The probability of desired changes occurring and passing the trait in the next generation is high, so it takes 1-3 generations to establish a breed. Moreover, CRISPR/Cas genome editing rapidly introduces favorable changes by disrupting genes with targeted minor changes, in contrast to transgenesis, which introduces foreign genes into the host genome and thereby alleviates major public concerns on safety. Although the CRISPR/Cas technology has a tremendous potential, there are several technical challenges and regulatory and public issues concerning the applications in fisheries and the aquaculture breeding sector. Nonetheless, the exciting point in the CRISPR/Cas9 genome editing is that two CRISPR-edited fish, namely, red sea bream and tiger puffer developed by the Kyoto-based startup got approval and are now on the market for sale, and another fish, FLT-01 Nile tilapia developed by the AquaBounty, is not classified under genetically modified organism regulatory. However, there is still a way to go before it revolutionizes and becomes viable in commercial aquaculture as the new breeding technology for aquaculture-important traits and species.

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Section: Advances Of Crispr/cas-genome Editing In Aquaculture and Fis...mentioning

confidence: 99%

Section: Technical Challengesmentioning

confidence: 99%

Section: Technical Challengesmentioning

confidence: 99%

Section: Regulatory and Consumer Acceptancementioning

confidence: 99%

See 2 more Smart Citations

CRISPR/Cas Genome Editing—Can It Become a Game Changer in Future Fisheries Sector?

Roy

Kumar²,

Behera³

et al. 2022

Front. Mar. Sci.

View full text Add to dashboard Cite

show abstract

“…Genome editing is an efficient technology in functional genomics to assess the effects of causative genes and variants on traits of interest and may also be a promising targeted breeding technology to expedite sustainable genetic improvement and innovation. This genetic tool can quickly fix target alleles in a population, even in a single generation, improve introgression from alleles in other populations or even create de novo alleles 115,223,224 . Genome editing has been used in mammals, and there has been detailed discussion of the availability of editing target genes in vivo and in cell lines of several aquaculture species 115,223 .…”

Section: Future Perspectives For Common Carp Genetic Improvementmentioning

confidence: 99%

Research advances and future perspectives of genomics and genetic improvement in allotetraploid common carp

Chen

Sun

et al. 2021

Reviews in Aquaculture

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Aquatic species have become an integral part of human culture. As an internationally important food fish species, the common carp Cyprinus carpio accounted for over 7% of global aquaculture production in 2020. In addition to serving as a food source, common carp varieties are used as ornamental fish and valued in recreational fisheries. The continuously updated reference genome of common carp has recently served as a foundation for both basic genetic studies and evolutionary studies. With the recent emphasis on the allotetraploid signature of common carp, researchers have begun to focus on how hybridization and polyploidization affect the successful speciation and advantageous behaviour of common carp. In this review, we survey current and emerging research topics on common carp, including genomic and genetic tools, germplasm resources, genetic diversity, genetic mechanism and improvement of economic traits, as well as multiple breeding technologies employed in common carp. We also provide an overview of recent trends in genetic studies and improvements in common carp and encourage researchers to monitor the allotetraploid signature of common carp. Understanding the genetic basis of economically important traits and the mechanisms of allopolyploidization in common carp will be important for genetic improvement and germplasm innovation in agronomically important fish species, although the road ahead will be challenging for this widely domesticated allotetraploid species.

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