Genome composition of ‘Elatior’-begonias hybrids analyzed by genomic in situ hybridisation

Marasek-Ciołakowska, Agnieszka; Ramanna, M.S.; Laak, W. A. ter; Tuyl, J.M. van

doi:10.1007/s10681-009-0057-7

Cited by 10 publications

(9 citation statements)

References 38 publications

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“…Some authors (Matsuura & Okuno, 1936;Matsuura & Okuno, 1943;Okuna & Nagai, 1953;Okuna & Nagai, 1954) have suggested x = 6, x = 7 and x = 13 as the basic chromosome number, where x=13 may be of secondary origin. By using genomic in situ hybridisation (GISH), Marasek-Ciolakowska (2010) concluded that x = 7 may be the basic chromosome number of B. socotrana. They based this conclusion on the presence of 7 B. socotrana chromosomes and 56 chromosomes derived from tuberous Begonia in Elatior hybrids.…”

Section: Chromosome Numbermentioning

confidence: 99%

The Origin of Diversity in Begonia: Genome Dynamism, Population Processes and Phylogenetic Patterns

Dewitte¹,

Twyford²,

Thomas³

et al. 2011

The Dynamical Processes of Biodiversity - Case Studies of Evolution and Spatial Distribution

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Section: Chromosome Numbermentioning

confidence: 99%

The Origin of Diversity in Begonia: Genome Dynamism, Population Processes and Phylogenetic Patterns

Dewitte¹,

Twyford²,

Thomas³

et al. 2011

The Dynamical Processes of Biodiversity - Case Studies of Evolution and Spatial Distribution

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“…Intergenomic recombination has not been observed in these hybrids. In begonia breeding, genome composition and ploidy estimation are key points for further approaches and this can be accomplished by GISH molecular cytogenetic tools [ 39 ].…”

Section: Genomic Constitutionmentioning

confidence: 99%

Application of Genomic In Situ Hybridization in Horticultural Science

Ramzan

Younis

Lim

2017

International Journal of Genomics

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Molecular cytogenetic techniques, such as in situ hybridization methods, are admirable tools to analyze the genomic structure and function, chromosome constituents, recombination patterns, alien gene introgression, genome evolution, aneuploidy, and polyploidy and also genome constitution visualization and chromosome discrimination from different genomes in allopolyploids of various horticultural crops. Using GISH advancement as multicolor detection is a significant approach to analyze the small and numerous chromosomes in fruit species, for example, Diospyros hybrids. This analytical technique has proved to be the most exact and effective way for hybrid status confirmation and helps remarkably to distinguish donor parental genomes in hybrids such as Clivia, Rhododendron, and Lycoris ornamental hybrids. The genome characterization facilitates in hybrid selection having potential desirable characteristics during the early hybridization breeding, as this technique expedites to detect introgressed sequence chromosomes. This review study epitomizes applications and advancements of genomic in situ hybridization (GISH) techniques in horticultural plants.

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“…Knowledge of the genome constitution of wide hybrids is important for further progress in breeding (Marasek-Ciolakowska et al 2010). GISH analysis is a powerful tool for discriminating chromosomes from different genomes and allows visualization of the genome constitution at the level of whole chromosomes in allopolyploids and wide hybrids (Ji et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…GISH analysis of wide hybrids has so far been carried out in a wide range of plant species: interspecific hybrids in Lilium ( Barba-Gonzalez et al 2004Khan et al 2009;Marasek et al 2004;Zhou et al 2007), Tulipa (Marasek et al 2006;Marasek and Okazaki 2008), and Begonia spp. (Marasek-Ciolakowska et al 2010); and intergeneric hybrids in Aloeaceae (Takahashi et al 1997), Solanaceae (Ji et al 2004) and Asteraceae (Deng et al 2010). However, GISH analysis has not yet been performed in Colchicaceae.…”

Section: Introductionmentioning

confidence: 99%

Genomic in situ hybridization (GISH) analysis of intergeneric hybrids in Colchicaceae

et al. 2011

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Some intergeneric hybrids produced by the crosses among several colchicaceous ornamentals, Gloriosa superba 'Lutea' (2n = 2x = 22), G. 'Marron Gold' (2n = 4x = 44), G. 'Verschild' (2n = 7x = 77), Littonia modesta Hook. (2n = 2x = 22), and Sandersonia aurantiaca Hook. (2n = 2x = 24), were subjected to genomic in situ hybridization (GISH) analysis in order to clarify their genome constitutions. Chromosome preparation was made from root tip cells of L. modesta 9 G. superba 'Lutea', L. modesta 9 S. aurantiaca, S. aurantiaca 9 G. superba 'Lutea', L. modesta 9 G. 'Marron Gold', S. aurantiaca 9 G. 'Marron Gold' and G. 'Verschild' 9 S. aurantiaca. Total DNA of one parent was labeled with digoxigenin or biotin and used as probe, and chromosomes were counterstained with 4 0 -6-diamidono-2-phenylindole (DAPI). For all the nine intergeneric hybrids, chromosomes from each parent could be clearly distinguished by GISH analysis. Thus GISH analysis is a powerful tool for identifying the genome constitution of intergeneric hybrids in colchicaceous ornamentals. The results obtained by GISH analysis study may be important for further progress in breeding of colchicaceous ornamentals.

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Genome composition of ‘Elatior’-begonias hybrids analyzed by genomic in situ hybridisation

Cited by 10 publications

References 38 publications

The Origin of Diversity in Begonia: Genome Dynamism, Population Processes and Phylogenetic Patterns

The Origin of Diversity in Begonia: Genome Dynamism, Population Processes and Phylogenetic Patterns

Application of Genomic In Situ Hybridization in Horticultural Science

Genomic in situ hybridization (GISH) analysis of intergeneric hybrids in Colchicaceae

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