Application of Genomic In Situ Hybridization in Horticultural Science

Ramzan, Fahad; Younis, Adnan; Lim, Ki-Byung

doi:10.1155/2017/7561909

Cited by 30 publications

(22 citation statements)

References 58 publications

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“…In situ hybridization, using the genomic DNA of one or two species as a probe, is an effective way of identifying chromosomes from different sources in interspecific hybrids (Ramzan et al 2017). It has often been used for characterizing somatic hybrids, especially from the families Brassicaceae (Tu et al 2008;Du et al 2009), Poaceae (Cai et al 2007), Solanaceae (Escalante et al 1998;Kulawiec et al 2003), or Amaryllidaceae (Buiteveld et al 1998b;Yamashita et al 2002).…”

Section: Cytogenetic Description Of Somatic Hybridsmentioning

confidence: 99%

Molecular and cytogenetic description of somatic hybrids between Gentiana cruciata L. and G. tibetica King

Tomiczak

2019

J Appl Genetics

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Somatic hybridization provides an opportunity to create cells with new genetic constitution. Here, the interspecific somatic hybrid plants regenerated in vitro following fusion of cell suspension-derived protoplasts of tetraploid Cross Gentian (Gentiana cruciata L., 2n = 52) with protoplasts released from mesophyll tissue of another tetraploid species, Tibetan Gentian (G. tibetica King, 2n = 52), were studied. According to the results of genome analyses with AFLP, ISSR, and CAPS markers, all somatic hybrids were genetically closer to "suspension" fusion partner G. cruciata than to "mesophyll" partner G. tibetica, but they got G. tibetica chloroplasts. Chromosome counting revealed little variation in the number of chromosomes in hybrid's cells (2n = 88 or 2n = 90), although all plants possessed similar nuclear DNA content which remained stable even after 2 years of in vitro culture. Fluorescence in situ hybridization (FISH) showed that hybrids possessed 4 to 7 chromosomes bearing 5S rDNA sites and 6 or 7 chromosomes with 35S rDNA sites. A part of FISH signals was smaller than those observed in the parental species, which could indicate the loss of rDNA sequences. Genomic in situ hybridization (GISH) showed the predominance of the number of G. cruciata chromosomes over chromosomes of G. tibetica. However, a significant level of cross-hybridization was observed for about one-third of hybrid chromosomes, indicating a high degree of homeology between the genomes of G. cruciata and G. tibetica.

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Section: Cytogenetic Description Of Somatic Hybridsmentioning

confidence: 99%

Molecular and cytogenetic description of somatic hybrids between Gentiana cruciata L. and G. tibetica King

Tomiczak

2019

J Appl Genetics

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“…Intergenomic recombination chromosomes containing chromosome segments from both parental species can be readily identified, and their behaviour and transmission can be monitored through meiosis into the progenies as shown in Lilium interspecific hybrids and Elymus dahuricus (Kwon et al, 2017;Yang et al, 2017). Ramzan et al (2017) has recently reviewed the application of GISH in horticultural plants and concluded that GISH has been used to perform chromosomal evaluation, cytogenetical classification, genomic constitution, polyploidy confirmation, hybrid verification and introgression breeding. In this study, the OxG hybrids were backcrossed using E. guineensis (tenera) as male parent for developing (GISH) method.…”

Section: Short Communication: Genomic Constitution Of Oil Palm Intersmentioning

confidence: 99%

SHORT COMMUNICATION: GENOMIC CONSTITUTION OF OIL PALM INTERSPECIFIC HYBRID CROSSES MONITORED BY GENOMIC in situ HYBRIDISATION (GISH)

Madon¹

2018

JOPR

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Interspecific hybridisation of Elaeis oleifera (O) and Elaeis guineensis (G) oil palm was done to create recombinant progenies, containing various composition of parental genetic material. Genomic in situ hybridisation (GISH) on OxG hybrids had previously shown presence of 16 E. oleifera and 16 E. guineensis chromosomes. In this study, GISH was used to examine the metaphase chromosomes of the OxG interspecific hybrids hybridised with E. guineensis [(OxG)xG] to produce backcross one (BC 1) progenies. Optimisation of unlabelled E. guineensis genomic DNA quantity were required to produce optimum discrimination of the E. oleifera and E. guineensis chromosomes. The amount needed was 120 µg indicating the high genome similarity. The chromosomes of BC 1 individuals were 2n=32 and consisted of variable composition of E. oleifera and E. guineensis chromosomes while the interphase nuclei showed regions of parental genomes in discrete, non-intermixed domains indicating non-random organisation of the nucleus. The seedlings were created to assist in GISH analysis, hence, only leaflet plane correlation with the number of E. oleifera chromosomes transmitted into the progenies were done. No correlation can be observed between the numbers of E. oleifera chromosomes and the leaflet plane.

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“…GISH analyses may facilitate the choice of promising hybrids during the early stages of breeding through the detection of alien chromatin. This characterization allows the planning of crosses, aiming to maximize the segregation for the recovery of superior genotypes (Fukuhara et al, 2016;Liu et al, 2017;Ramzan et al, 2017;Grewal et al, 2018;).…”

Section: Introductionmentioning

confidence: 99%

Genome composition and pollen viability of Jatropha (Euphorbiaceae) interspecific hybrids by Genomic In Situ Hybridization (GISH)

et al. 2019

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Interspecific hybridization is required for the development of Jatropha curcas L. improved cultivars, due to its narrow genetic basis. The present study aimed to analyze the parental genomic composition of F 1 and BC 1 F 1 generations derived from interspecific crosses (J. curcas/J. integerrima and J. curcas/J. multifida) by GISH (Genomic In Situ Hybridization), and the meiotic index and pollen viability of F 1 hybrids. In F 1 cells from both hybrids, 11 chromosomes of each parental was observed, as expected, but chromosome rearrangement events could be detected using rDNA chromosome markers, suggesting unbalanced cells. In the BC 1 F 1 , both hybrids had 22 chromosomes, suggesting that only n = 11 gametes were viable in the next generation. However, GISH allowed the identification of three and two alien chromosomes in J. curcas//J. integerrima and J. curcas//J. multifida BC 1 F 1 hybrids, respectively, suggesting a preferential transmission of J. curcas chromosomes for both hybrids. Pollen viability in F 1 hybrids derived from J. curcas/J. integerrima crosses were higher (82-83%) than those found for J. curcas/J. multifida (68%), showing post-meiotic problems in these last hybrids, with dyads, triads, polyads, and micronuclei as post-meiosis results. The here presented cytogenetic characterization of interspecific hybrids and their backcross progenies can contribute to the selection of the best genotypes for future assisted breeding of J. curcas.

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Application of Genomic In Situ Hybridization in Horticultural Science

Cited by 30 publications

References 58 publications

Molecular and cytogenetic description of somatic hybrids between Gentiana cruciata L. and G. tibetica King

Molecular and cytogenetic description of somatic hybrids between Gentiana cruciata L. and G. tibetica King

SHORT COMMUNICATION: GENOMIC CONSTITUTION OF OIL PALM INTERSPECIFIC HYBRID CROSSES MONITORED BY GENOMIC in situ HYBRIDISATION (GISH)

Genome composition and pollen viability of Jatropha (Euphorbiaceae) interspecific hybrids by Genomic In Situ Hybridization (GISH)

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