Genome characterization of novel lytic Myoviridae bacteriophage ϕVP-1 enhances its applicability against MDR-biofilm-forming Vibrio parahaemolyticus

Matamp, Nandita; Bhat, Sarita G.

doi:10.1007/s00705-019-04493-6

Cited by 28 publications

(15 citation statements)

References 45 publications

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“…A positive theoretical correlation is assumed between lysis time and burst size, that is, the longer the lysis time, the larger the burst size. This is corroborated by observing the vB_Pd_PDCC‐1 titration for the first 25 min and then remained in the plateau phase (Wang 2006; Matamp and Bhat 2019). However, a deeper understanding of the survival of phages under stress conditions is necessary for their application as biocontrol agents in different areas of aquaculture (Ackermann et al .…”

Section: Discussionmentioning

confidence: 56%

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Use of bacteriophage vB_Pd_PDCC‐1 as biological control agent of Photobacterium damselae subsp. damselae during hatching of longfin yellowtail ( Seriola rivoliana ) eggs

et al. 2020

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Aims This study describes the effect of phage therapy on hatching of longfin yellowtail (Seriola rivoliana) eggs challenged with Photobacterium damselae subsp. damselae. Methods and results A lytic phage (vB_Pd_PDCC‐1) against P. damselae subsp. damselae was isolated and characterized. The use of phage vB_Pd_PDCC‐1 increased the hatching rate of eggs, and reduced presumptive Vibrio species to non‐detectable numbers, even in non‐disinfected eggs. High‐throughput 16S rRNA gene sequencing analysis revealed that phage vB_Pd_PDCC‐1 caused significant changes in the composition and structure of the associated microbiota, allowing that members (e.g. those belonging to the family Vibrionaceae) of the class Gammaproteobacteria to be displaced by members of the class Alphaproteobacteria. Conclusions To the best of our knowledge, this represents the first study evaluating phage therapy to control potential negative effects of P. damselae subsp. damselae during hatching of longfin yellowtail eggs. Significance and Impact of the Study The Seriola genus includes several important commercial fish species due to its rapid growth and easy adaptability to confinement conditions. However, bacterial infections (especially those caused by Vibrio and Photobacterium species) are among the main limiting factors for the intensification of marine fish aquaculture, particularly during early development stages. Therefore, the use of phages, which are natural killers of bacteria, represents a promising strategy to reduce the mortality of farmed organisms caused by pathogenic bacteria.

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Section: Discussionmentioning

confidence: 56%

“…2010; Yamaki et al . 2015; Matamp and Bhat 2019). A positive theoretical correlation is assumed between lysis time and burst size, that is, the longer the lysis time, the larger the burst size.…”

Section: Discussionmentioning

confidence: 99%

Use of bacteriophage vB_Pd_PDCC‐1 as biological control agent of Photobacterium damselae subsp. damselae during hatching of longfin yellowtail ( Seriola rivoliana ) eggs

et al. 2020

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“…This study also elucidates that the phage is able to hijack protein translation machinery of the host bacteria. In comparison to other phages that infect vibrios (Table S2) [46][47][48][49][50][51][52] , phage Seahorse has relatively big burst size with a shorter latency period than phages VP-1, VP-2, VP-3, VpKK5, and VhKM4 that replicate in V. parahaemolyticus 27,29,[53][54][55][56] . It is also tolerant to the wide range of pHs and temperatures compared to other vibriophages suggesting unique survivability in harsh natural environments.…”

Section: Discussionmentioning

confidence: 95%

A novel vibriophage exhibits inhibitory activity against host protein synthesis machinery

Thammatinna

Egan

Htoo

et al. 2020

Sci Rep

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Since the emergence of deadly pathogens and multidrug-resistant bacteria at an alarmingly increased rate, bacteriophages have been developed as a controlling bioagent to prevent the spread of pathogenic bacteria. One of these pathogens, disease-causing Vibrio parahaemolyticus (Vp AHPND ) which induces acute hepatopancreatic necrosis, is considered one of the deadliest shrimp pathogens, and has recently become resistant to various classes of antibiotics. Here, we discovered a novel vibriophage that specifically targets the vibrio host, VP AHPND . The vibriophage, designated Seahorse, was classified in the family Siphoviridae because of its icosahedral capsid surrounded by head fibers and a non-contractile long tail. Phage Seahorse was able to infect the host in a broad range of pH and temperatures, and it had a relatively short latent period (nearly 30 minutes) in which it produced progeny at 72 particles per cell at the end of its lytic cycle. Upon phage infection, the host nucleoid condensed and became toroidal, similar to the bacterial DNA morphology seen during tetracycline treatment, suggesting that phage Seahorse hijacked host biosynthesis pathways through protein translation. As phage Seahorse genome encodes 48 open reading frames with many hypothetical proteins, this genome could be a potential untapped resource for the discovery of phage-derived therapeutic proteins.Vibrio is a genus of motile Gram-negative bacteria that possesses a curved-rod cell shape with a single flagellum. Vibrios are abundant and diverse bacteria that are typically found in marine habitats. The genus Vibrio consists of 14 recognized clades and at least 86 different species 1 . While some of them are not pathogenic, many can cause serious health effects in both human and aquatic life. Due to the continuously rising ocean temperature, the composition of vibrio in the ocean microbiome has been reported to be higher than usual 2-4 . This vibrio-rich environment might increase the incident of a vibrio outbreak in the near future posing risks to global health 2 .Vibrio parahaemolyticus, which is one of the disease-causing Vibrio species, is pathogenic to both humans and marine animals 5 . Consumption of raw seafoods contaminated with the bacteria can cause acute gastroenteritis 5,6 . This opportunistic bacterium is also able to infect through an open wound which can lead to sepsis and, in rare cases, subsequent death in immunocompromised patients 7,8 . Moreover, V. parahaemolyticus that has acquired a plasmid encoding the deadly binary toxins PirA vp /PirB vp is even more virulent 9 . The V. parahaemolyticus strain harboring the plasmid has been found to cause a newly emerging disease in shrimp, known as acute hepatopancreatic necrosis disease (AHPND) 9 . Moreover, the AHPND-causing plasmid is also found to be transferable among other vibrios, increasing the chance of the disease spreading regionally and globally 10 . Unsurprisingly, the spread of AHPND has been reported in many countries, including China, Vietnam, Malaysia, Thailand, Me...

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“…Similarly, several studies reported the isolation of Vibrio spp. bacteriophages from a similar source such as shrimp aquaculture environments (Alagappan et al, 2010;Stalin and Srinivasan, 2017;Matamp and Bhat, 2020). For instance, Alagappan et al (2010) reported that five bacteriophages with the ability to infect V. parahaemolyticus MTCC-451 strain were isolated from the shrimp pond.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Six Vibrio parahaemolyticus Lytic Bacteriophages From Seafood Samples

et al. 2021

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Vibrio parahaemolyticus is a foodborne pathogen that is frequently isolated from a variety of seafood. To control this pathogenic Vibrio spp., the implementation of bacteriophages in aquaculture and food industries have shown a promising alternative to antibiotics. In this study, six bacteriophages isolated from the seafood samples demonstrated a narrow host range specificity that infecting only the V. parahaemolyticus strains. Morphological analysis revealed that bacteriophages Vp33, Vp22, Vp21, and Vp02 belong to the Podoviridae family, while bacteriophages Vp08 and Vp11 were categorized into the Siphoviridae family. All bacteriophages were composed of DNA genome and showed distinctive restriction fragment length polymorphism. The optimal MOI for bacteriophage propagation was determined to be 0.001 to 1. One-step growth curve revealed that the latent period ranged from 10 to 20 min, and the burst size of bacteriophage was approximately 17 to 51 PFU/cell. The influence of temperature and pH levels on the stability of bacteriophages showed that all bacteriophages were optimally stable over a wide range of temperatures and pH levels. In vitro lytic activity of all bacteriophages demonstrated to have a significant effect against V. parahaemolyticus. Besides, the application of a bacteriophage cocktail instead of a single bacteriophage suspension was observed to have a better efficiency to control the growth of V. parahaemolyticus. Results from this study provided a basic understanding of the physiological and biological properties of the isolated bacteriophages before it can be readily used as a biocontrol agent against the growth of V. parahaemolyticus.

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Genome characterization of novel lytic Myoviridae bacteriophage ϕVP-1 enhances its applicability against MDR-biofilm-forming Vibrio parahaemolyticus

Cited by 28 publications

References 45 publications

Use of bacteriophage vB_Pd_PDCC‐1 as biological control agent of Photobacterium damselae subsp. damselae during hatching of longfin yellowtail ( Seriola rivoliana ) eggs

Use of bacteriophage vB_Pd_PDCC‐1 as biological control agent of Photobacterium damselae subsp. damselae during hatching of longfin yellowtail ( Seriola rivoliana ) eggs

A novel vibriophage exhibits inhibitory activity against host protein synthesis machinery

Isolation and Characterization of Six Vibrio parahaemolyticus Lytic Bacteriophages From Seafood Samples

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