2021
DOI: 10.1101/2021.03.15.435372
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Genome assembly of the maize inbred line A188 provides a new reference genome for functional genomics

Abstract: Heretofore, little is known about the mechanism underlying the genotype-dependence of embryonic callus (EC) induction, which has severely inhibited the development of maize genetic engineering. Here, we report the genome sequence and annotation of a maize inbred line with high EC induction ratio, A188, which is assembled from single-molecule sequencing and optical genome mapping. We assembled a 2,210 Mb genome with a scaffold N50 size of 11.61 million bases (Mb), compared to those of 9.73 Mb for B73 and 10.2 M… Show more

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“…Multiple maize reference genomes have been developed and well annotated (Ge et al., 2022; Haberer et al., 2020; Hirsch et al., 2016; Hufford et al., 2021; Jiao et al., 2017; Springer et al., 2018; Sun et al., 2018; Yang et al., 2019), and more are being developed every day. The genetic material used to create the reference genome may be different from that utilized in your experiment.…”
Section: Commentarymentioning
confidence: 99%
“…Multiple maize reference genomes have been developed and well annotated (Ge et al., 2022; Haberer et al., 2020; Hirsch et al., 2016; Hufford et al., 2021; Jiao et al., 2017; Springer et al., 2018; Sun et al., 2018; Yang et al., 2019), and more are being developed every day. The genetic material used to create the reference genome may be different from that utilized in your experiment.…”
Section: Commentarymentioning
confidence: 99%