2022
DOI: 10.1002/cpz1.591
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Mapping Maize Mutants Using Bulked‐Segregant Analysis and Next‐Generation Sequencing

Abstract: Forward genetics is used to identify the genetic basis for a phenotype. The approach involves identifying a mutant organism exhibiting a phenotype of interest and then mapping the causative locus or gene. Bulked‐segregant analysis (BSA) is a quick and effective approach to map mutants using pools of mutants and wild‐type plants from a segregating population to identify linkage of the mutant phenotype, and this approach has been successfully used in plants. Traditional linkage mapping approaches are outdated an… Show more

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