Genome assembly and annotation of <i>Periplaneta americana</i> reveal a comprehensive cockroach allergen profile

Wang, Lingyi; Xiong, Qing; Saelim, Nawannaporn; Wang, Lin; Nong, Wenyan; Wan, Angel Tsz-Yau; Shi, Mai; Liu, Xiaoyu; Cao, Qin; Hui, Jerome H.L.; Sookrung, Nitat; Leung, Ting Fan; Tungtrongchitr, Anchalee; Tsui, Stephen Kwok‐Wing

doi:10.1111/all.15531

Cited by 11 publications

(4 citation statements)

References 68 publications

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“…Analysis of the brain transcritome SRAs from the American cockroach stung by the sphecid wasp Ampulex compressa (Arvidson et al, 2019) yielded one more sirp than those found in the first genome assembly of P. americana (Li et al, 2018), however this gene is present in two more recent genome assemblies from this species (Wang et al, 2022; Zheng et al, 2022).…”

Section: Resultsmentioning

confidence: 95%

Different neuroendocrine cell types in thepars intercerebralisofPeriplaneta americanaproduce their own specific IGF-related peptides

Veenstra

2022

Preprint

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Of the nine genes of the American cockroach, Periplaneta americana, coding for peptides related to insulin and insulin-like growth factor, seven show significant expression in the central nervous system as demonstrated by the polymerase chain reaction on reverse transcribed RNA. In situ hybridisation shows that five of those are expressed by cells in the pars intercerebralis. Antisera raised to the predicted peptides show that these cells are neuroendocrine in nature and project to the corpora cardiaca. Interestingly, and in contrast to Drosophila where a single cell type expresses a number of genes expressing several such peptides, there are at least three cell types that each express different genes. Whereas in Drosophila the neuroendocrine cells producing insulin-like peptides also express sulfakinins, the arthropod orthologs of gastrin and cholecystokinin, in Periplaneta the sulfakinins are produced by different cells. Other neuropeptides known to be produced by the pars intercerebralis in this and other insect species, such as the CRF-like diuretic hormone, neuroparsin, leucokinin or myosuppressin, neither colocalize with an insulin-related peptide. The differential expression of these peptides and the existence of multiple insulin receptors in this species implies a more complex regulation by insulin and IGF-related peptides in cockroaches than in the fruit fly.

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Section: Resultsmentioning

confidence: 95%

Different neuroendocrine cell types in thepars intercerebralisofPeriplaneta americanaproduce their own specific IGF-related peptides

Veenstra

2022

Preprint

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“…The abundance of MA genes combined with the repeated sequences makes the assembly of transcripts and genes very difficult. The third Periplaneta genome assembly, obtained by a group working on cockroach allergens, still did not allow for their unambiguous assembly [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Evolution of a Cockroach Allergen into the Major Protein of Termite Royal Jelly

Veenstra

2023

IJMS

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Termites live in colonies, and their members belong to different castes that each have their specific role within the termite society. In well-established colonies of higher termites, the only food the founding female, the queen, receives is saliva from workers; such queens can live for many years and produce up to 10,000 eggs per day. In higher termites, worker saliva must thus constitute a complete diet and therein resembles royal jelly produced by the hypopharyngeal glands of honeybee workers that serves as food for their queens; indeed, it might as well be called termite royal jelly. However, whereas the composition of honeybee royal jelly is well established, that of worker termite saliva in higher termites remains largely unknown. In lower termites, cellulose-digesting enzymes constitute the major proteins in worker saliva, but these enzymes are absent in higher termites. Others identified a partial protein sequence of the major saliva protein of a higher termite and identified it as a homolog of a cockroach allergen. Publicly available genome and transcriptome sequences from termites make it possible to study this protein in more detail. The gene coding the termite ortholog was duplicated, and the new paralog was preferentially expressed in the salivary gland. The amino acid sequence of the original allergen lacks the essential amino acids methionine, cysteine and tryptophan, but the salivary paralog incorporated these amino acids, thus allowing it to become more nutritionally balanced. The gene is found in both lower and higher termites, but it is in the latter that the salivary paralog gene got reamplified, facilitating an even higher expression of the allergen. This protein is not expressed in soldiers, and, like the major royal jelly proteins in honeybees, it is expressed in young but not old workers.

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“…In brief, the protocol steps included: chopping the tissue into tiny pieces using a sterilized razor blade with a few drops of 40% glacial acetic then leaving it for 48 hours in the dark; immersing the processed slides into th fixation reagent (85:10:5 volumes of methanol:formaline:acetic acid); then hydrolysing them (using hydrochloric acid) and staining them (using Schiff’s reagent). We used three standards of known C-values: Periplaneta americana (3.41 pg) (61), Lasius niger (0.33 pg) (JF Flot, unpublished data), and Ambystoma mexicanum (32 pg) (62). A digital camera (5 megapixels) mounted on a compound microscope was used for imaging the slides, and nuclei measurements were performed using ImageJ (63).…”

Section: Methodsmentioning

confidence: 99%

First chromosome-level genome assembly of the colonial tunicateBotryllus schlosseri

De Thier,

M.Tawfeeq,

Faure

et al. 2024

Preprint

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Botryllus schlosseri(Tunicata) is a colonial chordate that has long been studied for its multiple developmental pathways and regenerative abilities and its genetically determined allorecognition system based on a polymorphic locus that controls chimerism and cell parasitism. We present the first chromosome-level genome assembly from an isogenic colony ofB. schlossericlade A1 using a mix of long and short reads scaf-folded using Hi-C. This haploid assembly spans 533 Mb, of which 96% are found in 16 chromosome-scale scaffolds. With a BUSCO completeness of 91.2%, this complete and contiguousB. schlosserigenome assembly provides a valuable genomic resource for the scientific community and lays the foundation for future investigations into the molecular mechanisms underlying coloniality, regeneration, histocompatibility, and the immune system in tunicates.

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Genome assembly and annotation of Periplaneta americana reveal a comprehensive cockroach allergen profile

Cited by 11 publications

References 68 publications

Different neuroendocrine cell types in thepars intercerebralisofPeriplaneta americanaproduce their own specific IGF-related peptides

Different neuroendocrine cell types in thepars intercerebralisofPeriplaneta americanaproduce their own specific IGF-related peptides

Evolution of a Cockroach Allergen into the Major Protein of Termite Royal Jelly

First chromosome-level genome assembly of the colonial tunicateBotryllus schlosseri

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