Genital infection in mares

Crowhurst, R. C.

doi:10.1136/vr.100.22.476-a

Cited by 100 publications

(56 citation statements)

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“…Since the first reported outbreaks in 1977 in England [7] and Ireland [23], CEM has spread worldwide [15,22] and remains a considerable economical problem among horse breeders in some countries including Europe [25] and Japan. The causative agent of CEM, Taylorella equigenitalis, is a gramnegative, nonmotile, coccoid rod which is classified in the family Pasteurellaceae and whose closest phylogenetic relative is Oligella urethralis [2,4,5,16].…”

Section: Bacteria and Culture Conditionsmentioning

confidence: 99%

Development of a PCR Test for Rapid Diagnosis of Contagious Equine Metritis.

et al. 1999

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ABSTRACT. In order to establish a rapid diagnostic method for contagious equine metritis (CEM), we developed and evaluated a polymerase chain reaction (PCR) test. Species-specific PCR primer sets were derived from the DNA sequence of a cloned DNA fragment of Taylorella equigenitalis that did not hybridize with the genome of a taxomonically related species, Oligella urethralis. Single step PCR with primer set P1-N2 and two-step semi-nested PCR with primer sets P1-N2 and P2-N2 detected as low as 100 and 10 CFU of the bacteria, respectively. Single-step PCR detected T. equigenitalis from genital swabs of experimentally infected mares with sensitivity comparable to that of bacterial isolation. Furthermore, two-step PCR was more sensitive than the culture method. Upon examination of field samples, 12 out of 3,123 samples were positive by single-step PCR while only 2 were positive by bacterial culture. The 12 PCRpositive samples originated from 5 mares, of which 3 animals were considered to be carriers based on previous bacteriologic and serologic diagnoses for CEM. The PCR test described in this study would provide a specific and highly sensitive tool for the rapid diagnosis of CEM.-KEY WORDS: contagious equine metritis, equine, metritis, polymerase chain reaction, Taylorella equigenitalis.

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Section: Bacteria and Culture Conditionsmentioning

confidence: 99%

Development of a PCR Test for Rapid Diagnosis of Contagious Equine Metritis.

et al. 1999

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“…Originally CEM was first reported in England in 1977 [4]. Since then, CEM and its causative agent have been detected in many countries and in various breeds of horses.…”

Section: Introductionmentioning

confidence: 99%

Possible misidentification of Bacteroides sp., probably B. ureolyticus as Taylorella equigenitalis: Implications for the laboratory diagnosis of CEM

Moore¹,

Millar²,

Xu³

et al. 2001

Vet. Res.

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-A wild-type isolate with similar morphological and phenotypic properties to Taylorella equigenitalis, the causative bacterial agent of contagious equine metritis (CEM), was referred for molecular identification by PCR amplification of the 16S rRNA gene. A species-specific PCR failed to yield a product compatible with that of T. equigenitalis. The direct sequencing of the universal 16S rRNA PCR amplicon suggested the presence of a Bacteroides sp., probably Bacteroides ureolyticus, with no consequent effects on the movement and transportation of the animal. Adoption of such a molecular means of identification through sequencing may aid in the identification of the atypical forms of Taylorella equigenitalis, as recently described, as well as differentiating this species from Taylorella asinigenitalis. horse / CEM / PCR / Taylorella equigenitalis / BacteroidesRésumé -Possibilité d'erreur dans l'identification d'un Bacteroides sp., probablement B. ureolyticus, détecté comme Taylorella equigenitallis : conséquences pour le diagnostic en laboratoire de la métrite contagieuse équine. Un isolat de type sauvage ayant des caractéristiques morphologiques et phénotypiques similaires à celles de Taylorella equigenitallis, l'agent bactérien responsable de la métrite contagieuse équine, a été soumis pour identification moléculaire par la technique d'amplification en chaîne par la polymérase (PCR) du gène de l'ARNr 16S. Une PCR spécifique à l'espèce n'a pas permis d'obtenir un produit compatible avec celui de T. equigenitalis. Le séquençage direct de l'amplicon de PCR de l'ARNr 16S universel suggère la présence d'un Bacteroides sp., probablement Bacteroides ureolyticus, n'ayant aucune conséquence sur le mouvement et le transport de l'animal. L'adoption d'une telle technique moléculaire d'identification par le séquençage pourrait aider à l'identification de formes atypiques de Taylorella equigenitalis qui ont été récemment décrites, ainsi que pour différencier cette espèce de Taylorella asinigenitalis. cheval / amplification en chaîne par polymérase / métrite contagieuse équine / Bacteroides Vet. Res. 32 (2001) 611-616 611

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“…It was first reported as a previously undescribed disease of horses in the United Kingdom and Ireland (Crowhurst, 1977;Platt et al, 1977), where it ravaged the Thoroughbreds racing industry in the late 70's and early 80's. Since then, the disease has been recorded in various horse breeds world-wide (Timoney, 1996;Ozgur et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Contagious equine metritis in Portugal: A retrospective report of the first outbreak in the country and recent contagious equine metritis test results

Rocha¹

2017

Open Vet J.

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Contagious equine metritis (CEM), a highly contagious bacterial venereal infection of equids, caused by Taylorella equigenitalis, is of major international concern, causing short-term infertility in mares. Portugal has a long tradition of horse breeding and exportation and until recently was considered CEM-free. However, in 2008, T. equigenitalis was isolated at our laboratory from a recently imported stallion and 2 mares from the same stud. Following this first reported outbreak, the Portuguese Veterinary Authority (DGVA) performed mandatory testing on all remaining equines at the stud (n=30), resulting in a further 4 positive animals. All positive animals were treated and subsequently tested negative for T. equigenitalis. Since this outbreak, over 2000 genital swabs from Portuguese horses have been tested at our laboratory, with no further positive animals identified. The available data suggests that this CEM outbreak was an isolated event and we have no further evidence of CEM cases in Portugal, however, an extended and wider epidemiological study would be needed to better evaluate the incidence of the disease in Portuguese horses.

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Genital infection in mares

Cited by 100 publications

References 0 publications

Development of a PCR Test for Rapid Diagnosis of Contagious Equine Metritis.

Development of a PCR Test for Rapid Diagnosis of Contagious Equine Metritis.

Possible misidentification of Bacteroides sp., probably B. ureolyticus as Taylorella equigenitalis: Implications for the laboratory diagnosis of CEM

Contagious equine metritis in Portugal: A retrospective report of the first outbreak in the country and recent contagious equine metritis test results

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