Genistein Reverses Changes of the Proteome Induced by Oxidized-LDL in EA·hy 926 Human Endothelial Cells

Abstract

Endothelial cells are primary targets for pro-atherosclerotic stressors such as oxidized LDL (ox-LDL). The isoflavone genistein, on the other hand, is suggested to prevent a variety of processes underlying atherosclerosis and cardiovascular diseases. By analyzing the proteome of EA.hy 926 endothelial cells, here we show, that genistein reverses the ox-LDL-induced changes of the steady-state levels of several proteins involved in atherosclerosis. These alterations caused by genistein are functionally linked to the inhibition of ox-LDL induced apoptosis.

Does genistein lower plasma lipids and homocysteine levels in postmenopausal women? A meta-analysis

“…Due to its potential cardioprotective role and favorable safety profile, genistein has received substantial attention over the last few years. It has been revealed that genistein not only affects the expression of atherosclerosis-relevant genes but also inhibits lesion and thrombus formations caused by endothelial stressors such as oxidized LDL or homocysteine [27][28][29][30] . In addition, genistein is effective in ameliorating cardiovascular profiles in experimental models of postmenopausal metabolic syndrome 31 .…”

Potential anti-inflammatory, anti-adhesive, anti/estrogenic, and angiotensin-converting enzyme inhibitory activities of anthocyanins and their gut metabolites

“…S. Edgell, University of North Carolina at Chapel Hill. EA.hy 926 cells were generated by fusion of human umbilical vein endothelial cells (HUVEC) with the human lung carcinoma cell line A549 and have been extensively used as a cell model for endothelial function (Fuchs et al 2005). Cells were grown in DMEM medium containing 4.5 g/L glucose and L-glutamine and supplemented with 10% FBS and 1% penicillin/streptomycin (5,000 U/mL).…”

Food Proteomics: Mapping Modifications

“…Proteomic studies have even been used to back up claims of the health benefits of soy (Erickson, 2005). In a recent study, an isoflavone from soybean has been found to prevent the regulation of proteins that induce apoptosis, cells which are the prime targets of arteriosclerotic stressors such as oxidised low density lipoproteins (Fuchs et al, 2005).…”