Genistein attenuates D-galactose-induced oxidative damage through decreased reactive oxygen species and NF-κB binding activity in neuronal PC12 cells

Hsieh, Huei-Min; Wu, Wen-Mein; Hu, Miao‐Lin

doi:10.1016/j.lfs.2010.10.021

Cited by 41 publications

(30 citation statements)

References 32 publications

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“…To evaluate effect of CG on protecting cells from OS, the ratio of SOD, and MDA, SOD/MDA, was determined to evaluate cell OS damage in general. In line with previous reports that phytoestrogen can protect cells from OS damage [Hsieh et al, 2011], here we observed that CG reversed AGEs-induced OS in HUVECs.…”

Section: Discussionsupporting

confidence: 93%

Phytoestrogen calycosin-7-O-β-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage

Xu¹,

Feng²,

Wang³

et al. 2011

J. Cell. Biochem.

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Vasculopathy including endothelial cell (EC) apoptosis and inflammation contributes to the high incidence of stroke and myocardial infarction in diabetic patients. The aim of the present study was to investigate the effect of calycosin-7-O-β-D-glucopyranoside (CG), a phytoestrogen, on advanced glycation end products (AGEs)-induced HUVEC damage. We observed that CG can significantly ameliorate AGEs-induced HUVEC oxidative stress and apoptosis. The ratio of SOD/MDA was significantly increased to the normal level by CG pretreatment. CG preincubation dramatically increased anti-apoptotic Bcl-2 while decreased pro-apoptotic Bax and Bad expressions as detected by immunocytochemistry. Moreover, CG ameliorated macrophage migration and adhesion to HUVEC; the monocyte chemotactic protein-1 and interleukin-6 levels in the culture supernatant were dramatically reduced by CG as determined by ELISA; the expressions of inflammatory proteins including ICAM-1, TGF-β1, and RAGE in both protein and mRNA levels were significantly reduced to the normal level by CG pretreatment as determined by immunocytochemistry and real-time RT-PCR. The intracellular investigation suggests that CG can reverse AGEs-activated ERK1/2 and NF-κB phosphorylation, in which estrogen receptors were involved in. Our results strongly indicate that CG can modulate EC dysfunction by ameliorating AGEs-induced cell apoptosis and inflammation.

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Section: Discussionsupporting

confidence: 93%

Phytoestrogen calycosin-7-O-β-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage

Xu¹,

Feng²,

Wang³

et al. 2011

J. Cell. Biochem.

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“…2 and 3). D-Gal injection to induce oxidative stress in model animals was employed previously for studying ageing-related changes due to increased production of free radicals and decreased antioxidant enzyme activities (Hsieh, Wu, & Hu, 2011). In this study, administration with L. plantarum C88 was found to improve the antioxidant status of the D-gal-induced oxidative stress mice in terms of the MDA levels, GSH-Px activities and T-AOC activities in liver, and serum SOD activities (Table 3).…”

Section: Discussionmentioning

confidence: 56%

Antioxidant activity of Lactobacillus plantarum strains isolated from traditional Chinese fermented foods

et al. 2012

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“…According to previous data, however, inflammation-inducing agents such as Aβ trigger NF-κB activation in astrocytes (Gonzalez-Velasquez et al, 2011) leading to inflammatory response, and this activation can be inhibited by genistein (Hsieh et al, 2011).…”

Section: Inos Nnos and Astrogliosismentioning

confidence: 88%

Genistein inhibits aggregation of exogenous amyloid-beta1–40 and alleviates astrogliosis in the hippocampus of rats

et al. 2012

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We addressed the question of whether injection of Aβ in the rat brain is associated with pathology in the hippocampus, and if genistein has any protective effect against the neuronal damage caused by Aβ 1-40 . Genistein is a plant-derived compound with a structure similar to that of the female sex hormone oestrogen and it was recently shown that pretreatment with a single dose of genistein ameliorated learning and memory deficits in an amyloid beta (Aβ) 1-40 rat model of Alzheimer's disease. Here, we report that injection of the amyloid peptide into the hippocampus of rats led to formation of Aβ 1-40 positive aggregates close to the lateral blade of the dentate gyrus (DGlb). We also observed the following in the hippocampus: extensive cell death in the DGlb (P < 0.0001), CA1 (P = 0.03), and CA3 (P = 0.002); an increased number of iNOS-expressing cells (P = 0.01) and gliosis. Genistein given to rats by gavage one hour before Key words: amyloid-beta, Alzheimer's disease, genistein, neuronal degeneration 1 1 Abbreviations: AD, Alzheimer's disease; iNOS, inducible nitric oxide synthase; nNOS, neuronal nitric oxide synthase; CA1, cornu ammonis 1; CA2, cornu ammonis 2; CA3, cornu ammonis 3; Aβ, amyloid beta; DGlb, lateral blade of dentate gyrus; DGmb, medial blade of dentate gyrus; GFAP, glial fibrillary acidic protein; CC, corpus callosum; MAP, mitogenactivated protein; NFĸB, nuclear factor kappa B; MnSOD, manganese superoxide dismutase; APP, amyloid precursor protein; CrEL, Cremophor EL

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Genistein attenuates D-galactose-induced oxidative damage through decreased reactive oxygen species and NF-κB binding activity in neuronal PC12 cells

Cited by 41 publications

References 32 publications

Phytoestrogen calycosin-7-O-β-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage

Phytoestrogen calycosin-7-O-β-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage

Antioxidant activity of Lactobacillus plantarum strains isolated from traditional Chinese fermented foods

Genistein inhibits aggregation of exogenous amyloid-beta1–40 and alleviates astrogliosis in the hippocampus of rats

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