Genic heterozygosity maintained by chromosomal interchanges in rye

Figueiras, A. M.; González-Jaén, M. Teresa; Candela, M.; Benito, C.

doi:10.1038/hdy.1988.8

Cited by 6 publications

(7 citation statements)

References 26 publications

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“…Several studies have documented both enzymatic and chromosomal polymorphisms within rye cultivars (Ramiriz & Pisabarro, 1986 ;Adam et al ., 1987 ;Lelley et al ., 1978 ;Figueiras et al ., 1988) . Such primers would result in polymorphic amplification profiles between cultivars .…”

Section: Discussionmentioning

confidence: 99%

Stability of RAPD markers for determining cultivar specific DNA profiles in rye (Secale cereale L.)

Iqbal

Rayburn

1994

Euphytica

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Cultivar specific DNA profiles in rye were revealed by polymerase chain reaction (PCR) using randomly amplified polymorphic DNA (RAPD) sequences . Ten base primers were used for the amplification of genomic DNA of rye cultivars by PCR . RAPD analysis was found to be reproducible among samples between PCR runs . When amplification profiles of different rye cultivars were compared using various primers, the overall profiles were cultivar specific . However, not all primers revealed polymorphisms . These primers appear to amplify conserved sequences in all rye cultivars . Intracultivar studies were conducted on two of the cultivars . In the cultivar Imperial, no polymorphisms were observed among ten plants analyzed with five primers . In the cultivar Balboa, polymorphisms were observed among fifty plants with four of the ten primers analyzed . Despite the small amount of intracultivar variability, RAPD analysis has the potential to be a rapid and reliable method of cultivar identification in this outcrossing species .

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Section: Discussionmentioning

confidence: 99%

Stability of RAPD markers for determining cultivar specific DNA profiles in rye (Secale cereale L.)

Iqbal

Rayburn

1994

Euphytica

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“…On the other hand, no significant relationship was found Figueiras et al (1985Figueiras et al ( , 1988Figueiras et al ( , 1991b, Benito et al (1991a, b).…”

Section: Resultsmentioning

confidence: 76%

“…The effect of background genotype (family or cross effect) on the five fitness components was studied in the two types of crosses by ANOVA analyses taking also into account the karyotype in the 1IV+5II Â 7II crosses (two way ANOVA). Allozyme genotypes were analyzed using a single leaf from each plant, following the protocols of Figueiras et al (1985Figueiras et al ( , 1988. The allozyme systems studied and the chromosomal location of the different loci are listed in Table 2.…”

Section: Methodsmentioning

confidence: 99%

“…On the other hand, the analysis of allozyme loci of several rye cultivars revealed a higher frequency of polymorphic loci, heterozygosity per locus and mean heterozygosity per cultivar in those cultivars of rye having translocations, including Aile´s, than in non-polymorphic ones (Figueiras et al, 1988). This fact could concur with the Burnham's hypothesis which proposed that the maintenance of interchange heterozygosity in some populations could be due to some advantage of this chromosome condition, since it could preserve genic heterozygosity in translocation heterozygotes at the interstitial segments due to the totally or partially suppressed crossing-over in these segments (Burnham, 1962).…”

Section: Introductionmentioning

confidence: 96%

“…This cultivar is allogamous and has always been maintained as a large panmictic population, with a highly developed self-incompatibility system and very low percentage of self-fertilization (Pe´rez de la Vega & Allard, 1984) and a heterozygosity level, estimated by allozyme loci, of 0.19 (Figueiras et al, 1988). Three different issues are considered: (i) the effects of interchange heterozygosity on fitness components; (ii) the correlations of these components with the number of heterozygous allozyme loci in both interchange heterozygous and standard homozygous plants and (iii) the effect of the genotype of each allozyme locus on fitness in structural heterozygotes and standard homozygotes.…”

Section: Introductionmentioning

confidence: 99%

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Genic heterozygosity, chromosomal interchanges and fitness in rye: any relationship?

et al. 2006

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Relationship between heterozygosity at allozyme loci, chromosomal interchanges and fitness was analyzed in a rye cultivar showing a polymorphism for such rearrangements. Nine allozyme systems (ACO, ACPH, GOT, GPI, LAP, MDH, PER, PGD and PGM) and five components of fitness (number of fertile tillers, total offspring, egg cell fertility, flowers/ear and seeds/ear) were studied. The estimated selection coefficients against interchange heterozygotes ranged from s = 0.12 to s = 0.34. A significant effect of the genic heterozygosity on some fitness components was observed in interchange heterozygotes (tillering and total offspring), in their standard homozygous sibs (flowers/ear and seeds/ear) and in the descendants of the crosses between standard karyotypes (flowers/ear, seeds/ear and egg cell fertility). However, the main effect was linked to genetic background associated to different crosses. Significant differences for Acph-1, Gpi-1, Lap-1, Mdh-1, Mdh-4, Pgd-2 and Pgm-1 loci were also found in some of these crosses although these differences were inconsistent. This suggests that probably the allozyme loci analyzed were not directly contributing to the fitness and that they are linked, in some cases, to different deleterious alleles depending on both cross and locus. This fact could support the local effect hypothesis as explanation although we do not discard the existence of some inbreeding level (general effect hypothesis) since all crosses and loci studied show a overall consistent trend of increased fitness with increased heterozygosity.

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Association of four isozyme loci with a reciprocal translocation between 1R/4R chromosomes in cultivated rye (Secale cereale L.)

Figueiras

Elorrieta

Benito

1989

Theoret. Appl. Genetics

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The progeny of four crosses between a structural heterozygote for a reciprocal translocation and a homozygote for the standard chromosome arrangement were analyzed in rye (Secale cereale L. cv "Ailés") for the electrophoretic patterns of eight different leaf and endosperm isozymes and also for the meiotic configuration at metaphase I. The Pgi-1, 6-Pgd-2 and Mdh-1 loci are linked to each other and also to the reciprocal translocation. These loci have been located on chromosome 1R. The Mdh-1 locus is located in the interstitial segment of chromosome 1R, between the centromere and the breakpoint. The Pgm-1 locus has been located on chromosome arm 4RS and is linked to Pgi-1, 6-Pgd-2, Mdh-1 and the reciprocal translocation. The estimated distance between the Pgm-1 locus and the centromere is 14.98 ± 2.27 cM. Therefore, the reciprocal translocation involves the 1R and 4R chromosomes. Other linked loci detected have been Mdh-2b and Est-2 (7.40 ± 2.90 cM) and Got-3 and Est-2 (5.62 ± 3.07 cM). These three last loci are located on chromosome 3R and their order most probably is Mdh-2b - Est-2 - Got-3.

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Genic heterozygosity maintained by chromosomal interchanges in rye

Cited by 6 publications

References 26 publications

Stability of RAPD markers for determining cultivar specific DNA profiles in rye (Secale cereale L.)

Stability of RAPD markers for determining cultivar specific DNA profiles in rye (Secale cereale L.)

Genic heterozygosity, chromosomal interchanges and fitness in rye: any relationship?

Association of four isozyme loci with a reciprocal translocation between 1R/4R chromosomes in cultivated rye (Secale cereale L.)

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