Stability of RAPD markers for determining cultivar specific DNA profiles in rye (Secale cereale L.)

Iqbal, Muhammad Javed; Rayburn, A. Lane

doi:10.1007/bf00025606

Cited by 30 publications

(14 citation statements)

References 17 publications

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“…Random amplified polymorphic DNA (RAPD) markers (Williams et al 1990) have been successfully used for cultivar analysis and species identification in many plants, due to the technical simplicity and speed of the RAPD methodology (Gepts 1993). The species include papaya (Carica papaya) (Stiles et al 1993), rye (Secale cereale L.) (Iqbal and Rayburn 1994), cotton (Multani and Lyon 1995;Khan et al 2000), lentil (Sharma et al 1995), Phaseolus vulgaris (Yonghe Bai et al 1998), Cicer (Ahmad 1999), Perilla crops (Nitta and Ohnishi 1999), buckwheat (Tsuji and Ohnishi 2000), groundnut (Subramanian et al 2000), tea (Camellia sinensis var. sinensis) (Kaundun et al 2000) and Lachenalia bulbifera (Kleynhans and Spies 2000).…”

Section: Introductionmentioning

confidence: 99%

Species relationships in Fagopyrum revealed by PCR-based DNA fingerprinting

Sharma

Jana

2002

Theor Appl Genet

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Random amplified polymorphic DNA (RAPD) markers were used to distinguish between 28 different accessions belonging to 14 species and two sub-species of Fagopyrum. Of the 75 random 10-mer primers tested, only 19 generated robust, easily interpretable amplification products. A total of 364 bands were observed with an average of 19.15 bands per primer, of which 99.45% were polymorphic. Primer OPN-08 produced the maximum number of fragments and UBC-183 produced the minimum number of fragments. The data were utilized to elucidate genetic relationships among 14 species and two sub-species of Fagopyrum. Cluster analysis using the unweighted paired group method of arithmetic means (UPGMA) showed four main clusters, two each of the cymosum and urophyllum groups. The results showed that Fagopyrum tataricum is closer to its wild ancestor F. tataricum ssp. potanini Batalin, closely followed by Fagopyrum giganteum. Cultivated common buckwheat ( Fagopyrum esculentum) showed affinity with its putative wild ancestor F. esculentum ssp. ancestrale and the other closely related diploid species Fagopyrum homotropicum. In the urophyllum group, Fagopyrum macrocarpum and Fagopyrum pleioramosum formed one cluster, whereas Fagopyrum capillatum, Fagopyrum gracilipes and Fagopyrum gilessii clustered separately. Except for a few cases, our results correspond with previously reported studies on Fagopyrum using the isozyme, RFLP and RAPD methods. Species-diagnostic amplification products specific to some species in the cymosum and urophyllum groups were identified. Our results show that RAPDs can be successfully used to analyze species relationships in Fagopyrum and also for constructing linkage maps.

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Section: Introductionmentioning

confidence: 99%

Species relationships in Fagopyrum revealed by PCR-based DNA fingerprinting

Sharma

Jana

2002

Theor Appl Genet

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“…Here, each variety consists of a population of genetically distinct individuals. In these cases, markers can either be used to differentiate varieties via distinctive marker allele combinations (Iqbal and Rayburn, 1994) or the differentiation of populations has to be expressed as genetic distances based on allele frequencies (Huff et al, 1993;Yu and Pauls, 1993).This, however, cannot rule out misinterpretations for small sample sizes. In each case, the discriminative power of molecular markers is much smaller than in vegetatively propagated plants.…”

Section: Variety and Genotype Identificationmentioning

confidence: 99%

Molecular Markers as a Tool for Analyses of Genetic Relatedness and Selection in Ornamentals

Debener

2002

Breeding for Ornamentals: Classical and Molecular Approaches

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“…Sources of reliability lie in the purity of the template DNA, magnesium (Mg 2+ ) concentration, the choice of thermal-stable DNA polymerase and thermal cycler used in PCR amplification. It also depends on the imprecise matches between short oligonucleotide primers (decamers) and the template DNA at the low annealing temperatures (35)(36)(37)(38)(39)(40) o C) of amplification conditions (Iqbal and Rayburn, 1994;Kelly, 1995;Qiu et al, 1995).…”

Section: Random Amplified Polymorphic Dna (Rapd)mentioning

confidence: 99%

Cassava Genetic Improvement

Ceballos¹,

Fregene²,

Carlos³

et al. 2007

Breeding Major Food Staples

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PREFACEThis Handbook is the result of a combined effort by several current and previous cassava researchers at CIAT to review and summarize the most important results of cassava research during the past 40 years. Most of the chapters are based on the various presentations during the Regional Cassava Training Course, held in Thailand from October 6 to 17, 2008. This course was organized upon the realization that many people that were actively involved with cassava research in the 1970s and 80s, both at CIAT and in national programs, have now retired or will soon do so, and that a whole new generation of cassava researchers are currently being hired to take their place.As cassava is now becoming a very important, and mostly industrial, crop in Asia, there are many new opportunities, but also a host of new problems and challenges. These include the appearance in Asia of new cassava diseases and pests; the decreasing availability and increasing cost of rural labor, resulting in the need for partial or complete mechanization of cassava production; the rapidly increasing demand for cassava roots for production of food, feed and fuel, and the unavailability in many countries of new land for any expansion of cassava area, thus requiring a rapid increase in cassava yields to increase supplies. This requires a renewed focus on cassava research for the development of new higher-yielding varieties and more sustainable production practices.While many cassava researchers in national programs in Asia received individual or group training at CIAT-Colombia during the 1970s and 80s, this training was greatly reduced during the following two decades due to funding limitations. Thus, the objective of the Regional Cassava Training Course in 2008 was to provide a new training opportunity for young scientists in Asian countries, and to hand over the knowledge and experience of the older cassava researchers, mostly from CIAT, to a new generation that will have to face the new challenges. Thus, the course was taught mostly by current or already retired CIAT cassava researchers, while the 60-plus participants of the course included cassava researchers from Cambodia, China, East Timor, India, Indonesia, Lao PDR, Malaysia, Myanmar, the Philippines, Thailand and Vietnam. The training course not only provided knowledge -from physiology and biotechnology to animal feeding and production of fuelethanol -but also an opportunity for people from different institutions and countries to get to know each other, which will greatly facilitate future collaboration.Course participants returned home with a CD containing the PowerPoint presentations of the course. However, it was felt that a more comprehensive review of all the topics covered was warranted, as this would provide more in-depth knowledge for those working in the various specialized fields. Most of this information is available in many refereed journals, in workshop proceedings and old CIAT annual reports, but many of these are now out of print or otherwise difficult to obtain. Thus, the Cas...

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Stability of RAPD markers for determining cultivar specific DNA profiles in rye (Secale cereale L.)

Cited by 30 publications

References 17 publications

Species relationships in Fagopyrum revealed by PCR-based DNA fingerprinting

Species relationships in Fagopyrum revealed by PCR-based DNA fingerprinting

Molecular Markers as a Tool for Analyses of Genetic Relatedness and Selection in Ornamentals

Cassava Genetic Improvement

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