GeneXpert Mycobacterium tuberculosis/rifampicin assay for molecular epidemiology of rifampicin-Resistant Mycobacterium tuberculosis in an Urban Setting of Banten province, Indonesia

Christopher, Paulus Mario; Cucunawangsih,; Widysanto, Allen

doi:10.4103/ijmy.ijmy_138_19

Cited by 13 publications

(9 citation statements)

References 47 publications

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“…The chances of tested as being false positive and false negative were also as low as 2.7% and 0.3% respectively. The results of current study are in line with previous studies conducted for the performance of GeneXpert diagnostic method [9,10].…”

Section: Discussionsupporting

confidence: 91%

“…GeneXpert MTB/RIF (Cepheid Sunnyvale, United States) is a novel revolutionary development in tuberculosis diagnostics with endorsement from WHO [6][7][8]. The accuracy of GeneXpert has been reported as high in many previous studies [9,10]. GeneXpert may be used for diagnosis of MTB and particularly rifampicin (RIF) resistant TB cases.…”

Section: Conventional Methods Of Diagnosis Including Sputum Microscopmentioning

confidence: 99%

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Evaluating the diagnostic accuracy of GeneXpert MTB/RIF assay for the diagnosis of Tuberculosis

Ullah

Lateef

et al. 2020

Preprint

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Background: Tuberculosis (TB) is one of the major global public health concern particularly affecting population of low-income countries. Early detection of disease coupled with other parameters help in treatment and reducing disease transmission. Methods: The current study was conducted to assess the sensitivity and specificity of the GeneXpert MTB/RIF (Cepheid Sunnyvale, CA, United States) in comparison to conventional techniques used for the diagnosis of TB. Our study is one of the first ones from Pakistan investigating and assessing the performance of GeneXpert. We recruited eight hundred clinically TB suspects initially and included seven hundred and sixteen clinically TB suspects in the final analysis. Results: The results of GeneXpert were compared with Mycobacteria Growth Indicator Tube (MGIT) and Ziehl-Neelsen (ZN) staining. In comparison to MGIT and ZN staining the sensitivity of GeneXpert with 95 % confidence interval (CI) was (99.7 %, CI 0.98-0.99) and (95.1 %, CI 0.92-0.97) respectively. The positive and negative predictive values with 95 % CI were (97.1 %, CI 0.94-0.98) and (99.7 %, CI 0.98-0.99) when results of GeneXpert were compare with MGIT results. Conclusion: The results of this study confirms the performance of GeneXpert. With high sensitivity and rapid detection, GeneXpert is ready to be considered as preferred diagnostic tool for TB.

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Section: Discussionsupporting

confidence: 91%

Section: Conventional Methods Of Diagnosis Including Sputum Microscopmentioning

confidence: 99%

Evaluating the diagnostic accuracy of GeneXpert MTB/RIF assay for the diagnosis of Tuberculosis

Ullah

Lateef

et al. 2020

Preprint

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“…All the research showed similar results compared to our research outcomes. These studies concluded that the application of the Gene-Xpert MTB/ RIF assay showed dramatic improvements for the rapid detection of tuberculosis and drug resistance, which offers promising advancements in TB control programs [53][54][55][56][57][58][59][60].…”

Section: Plos Onementioning

confidence: 99%

Diagnostic challenges and Gene-Xpert utility in detecting Mycobacterium tuberculosis among suspected cases of Pulmonary tuberculosis

et al. 2021

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The incidence of pulmonary tuberculosis (PTB) can be reduced by preventing transmission with rapid and precise case detection and early treatment. The Gene-Xpert MTB/RIF assay is a useful tool for detecting Mycobacterium tuberculosis (MTB) with rifampicin resistance within approximately two hours by using a nucleic acid amplification technique. This study was designed to reduce the underdiagnosis of smear-negative pulmonary TB and to assess the clinical and radiological characteristics of PTB patients. This cross-sectional study included 235 participants who went to the Luyang primary health care clinic from September 2016 to June 2017. The demographic data were analyzed to investigate the association of patient gender, age group, and ethnicity by chi-square test. To assess the efficacy of the diagnostic test, the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy were calculated. The area under the curve for sputum for both AFB and gene-Xpert was analyzed to compare their accuracy in diagnosing TB. In this study, TB was more common in males than in females. The majority (50.71%) of the cases belonged to the 25–44-year-old age group and the Bajau ethnicity (57.74%). Out of 50 pulmonary TB cases (smear-positive with AFB staining), 49 samples were positive according to the Gene-Xpert MTB/RIF assay and was confirmed by MTB culture. However, out of 185 smear-negative presumptive cases, 21 cases were positive by Gene-Xpert MTB/RIF assay in that a sample showed drug resistance, and these results were confirmed by MTB culture, showing resistance to isoniazid. In comparison to sputum for AFB, Gene-Xpert showed more sensitivity and specificity with almost complete accuracy. The additional 21 PTB cases detection from the presumptive cases by GeneXpert had significant impact compared to initial observation by the routine tests which overcame the diagnostic challenges and ambiguities.

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“…This study was reported by the Strengthening the Reporting of Cohort Studies in Surgery (STROCSS) 2019 guideline [ 12 ]. RR-TB is defined as TB resistant to rifampicin according to a molecular rapid test using GeneXpert MTB/RIF (Cepheid, Sunnyvale, CA, USA) [ 13 , 14 ]. RR-TB patients who are on intensive phase were defined as RR-TB patients who are diagnosed with RR-TB and start intensive phase of treatment with shorter regimens.…”

Section: Methodsmentioning

confidence: 99%

Correlation of inflammatory cytokines on corrected QT interval in rifampicin-resistant tuberculosis patients

Kusmiati

Mertaniasih

Putranto

et al. 2021

Annals of Medicine and Surgery

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Background The cases of Rifampicin-Resistant Tuberculosis (RR-TB) in our country have increased every year and RR-TB deaths are thought to be caused by prolongation of the QTc interval due to side effects of anti-tuberculosis drugs. Thus, cytokines are needed to be used as early markers of prolongation of the QTc interval in RR-TB patients. Objective This study aims to analyze the correlation of inflammatory cytokines on QTc interval in RR-TB patients who received shorter regimens. Methods This study uses a case-control study with a time series conducted in the period September 2019 to February 2020 in one of the referral hospitals for Tuberculosis in Indonesia. Cytokines levels from blood samples were measured using the ELISA method, while QTc intervals were automatically recorded using an electrocardiography machine. The statistical analysis used was the Chi-square test, Man Whitney test, Independence t -test, and Spearman-rank test with p < 0.05. Results There was no significant correlation between inflammatory cytokines and QTc prolongation in intensive phase which TNF-α value (6.8 pg/ml; r = 0.207; p = 0.281), IL-1β (20.13 pg/ml; r = 0.128; p = 0.509), and IL-6 (43.17 pg/ml; r = −0.028; p = 0.886). Meanwhile, in the continuation phase, the values for TNF-α (4.79 pg/ml; r = 0.046; p = 0.865), IL-1β (7.42 pg/ml; r = −0.223; p = 0.406), and IL- 6 (40.61 pg/ml; r = −0.147; p = 0.586). Conclusion inflammatory cytokines (TNF-α, IL-1β, and IL-6) cannot be used to identify QTc interval prolongation in RR-TB patients who received shorter regimens.

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GeneXpert Mycobacterium tuberculosis/rifampicin assay for molecular epidemiology of rifampicin-Resistant Mycobacterium tuberculosis in an Urban Setting of Banten province, Indonesia

Cited by 13 publications

References 47 publications

Evaluating the diagnostic accuracy of GeneXpert MTB/RIF assay for the diagnosis of Tuberculosis

Evaluating the diagnostic accuracy of GeneXpert MTB/RIF assay for the diagnosis of Tuberculosis

Diagnostic challenges and Gene-Xpert utility in detecting Mycobacterium tuberculosis among suspected cases of Pulmonary tuberculosis

Correlation of inflammatory cytokines on corrected QT interval in rifampicin-resistant tuberculosis patients

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