Genetics of Root System Architecture Using Near-isogenic Lines of Melon

Fita, Ana; Picó, Belén; Monforte, Antonio J.; Nuez, F.

doi:10.21273/jashs.133.3.448

Cited by 22 publications

(17 citation statements)

References 38 publications

(57 reference statements)

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“…Similar results were obtained in other tomato populations with the majority of the enhancing alleles for seed weight, fruit weight and total yield [13], and different botanical traits [66] coming from the S. lycopersicum parent. Our results are also supported by results in other crops in which QTLs were mainly affected by the positive allele of the parent with the heavy-weighted seed, for example in a study of the root architecture in melon [65]. Besides the observed strong positive correlation between seed dimensions and seedling traits, we also found co-location of QTLs for these traits, as might be expected from these results.…”

Section: Discussionsupporting

confidence: 91%

Exploring the Natural Variation for Seedling Traits and Their Link with Seed Dimensions in Tomato

et al. 2012

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The success of germination, growth and final yield of every crop depends to a large extent on the quality of the seeds used to grow the crop. Seed quality is defined as the viability and vigor attribute of a seed that enables the emergence and establishment of normal seedlings under a wide range of environments. We attempt to dissect the mechanisms involved in the acquisition of seed quality, through a combined approach of physiology and genetics. To achieve this goal we explored the genetic variation found in a RIL population of Solanum lycopersicum (cv. Moneymaker) x Solanum pimpinellifolium through extensive phenotyping of seed and seedling traits under both normal and nutrient stress conditions and root system architecture (RSA) traits under optimal conditions. We have identified 62 major QTLs on 21 different positions for seed, seedling and RSA traits in this population. We identified QTLs that were common across both conditions, as well as specific to stress conditions. Most of the QTLs identified for seedling traits co-located with seed size and seed weight QTLs and the positive alleles were mostly contributed by the S. lycopersicum parent. Co-location of QTLs for different traits might suggest that the same locus has pleiotropic effects on multiple traits due to a common mechanistic basis. We show that seed weight has a strong effect on seedling vigor and these results are of great importance for the isolation of the corresponding genes and elucidation of the underlying mechanisms.

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Section: Discussionsupporting

confidence: 91%

Exploring the Natural Variation for Seedling Traits and Their Link with Seed Dimensions in Tomato

et al. 2012

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“…These differences make these two groups suitable for constructing mapping populations. In fact, introgression lines derived from the cross of chi-SC and in-PS were successfully used to find QTLs associated with root architecture (Fita et al 2008b). However, plant size differences, especially in the first stages of development, constitute a serious constraint that makes it difficult to draw accurate conclusions regarding root genetics.…”

Section: Genetic Relationships Among Accessions and Root Architecturementioning

confidence: 99%

Diversity in root architecture and response to P deficiency in seedlings of Cucumis melo L.

Fita,

Nuez,

Picó

2011

Euphytica

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Breeding for more phosphorus (P)-efficient crops is one strategy to reduce the use of P fertilizers, thus mitigating the environmental and economic impacts of agriculture. Variation in root architecture and the response to P deficiency were studied in C. melo. Forty accessions representing genetic diversity within the species were screened for their root systems in normal and deficient P conditions at the seedling stage. Various parameters of P-uptake and P-use were analyzed in a subset of accessions at 40 days. Significant differences in root architecture were observed, with the taproot systems prevailing among the wild and exotic accessions, and more branched root systems in cultivated stocks. Moreover, differences in the plastic response of roots to P starvation were observed. Variation in different P-use and -uptake traits correlated with the root architecture. Within ssp. melo, the inodorus and flexuosus landraces had larger and more branched roots and more efficient P-uptake, thereby providing a close genepool for breeding. Within ssp. agrestis, conomon and momordica accessions can be sources of interest for the enhancement of variation in root architecture and P-use efficiency into cultivated melons. Therefore, the diversity observed within C. melo species could be useful in breeding P-efficient melon cultivars.

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“…melo varieties are dudaim (L.) Naudin, flexuosus (L.) Naudin, cantalupensis Naudin, and inodorus Jacquin, with cantalupensis and inodorus containing most of the commercial varieties [28]. Variability in root morphology and architecture has been described within this species, especially between varieties of both subspecies [29], [30]. The relationships between root architecture and response to Pi starvation has also been studied, showing a high variability in the acquisition and use of Pi among melon varieties [31].…”

Section: Introductionmentioning

confidence: 99%

Diversity in Expression of Phosphorus (P) Responsive Genes in Cucumis melo L

et al. 2012

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BackgroundPhosphorus (P) is a major limiting nutrient for plant growth in many soils. Studies in model species have identified genes involved in plant adaptations to low soil P availability. However, little information is available on the genetic bases of these adaptations in vegetable crops. In this respect, sequence data for melon now makes it possible to identify melon orthologues of candidate P responsive genes, and the expression of these genes can be used to explain the diversity in the root system adaptation to low P availability, recently observed in this species.Methodology and FindingsTranscriptional responses to P starvation were studied in nine diverse melon accessions by comparing the expression of eight candidate genes (Cm-PAP10.1, Cm-PAP10.2, Cm-RNS1, Cm-PPCK1, Cm-transferase, Cm-SQD1, Cm-DGD1 and Cm-SPX2) under P replete and P starved conditions. Differences among melon accessions were observed in response to P starvation, including differences in plant morphology, P uptake, P use efficiency (PUE) and gene expression. All studied genes were up regulated under P starvation conditions. Differences in the expression of genes involved in P mobilization and remobilization (Cm-PAP10.1, Cm-PAP10.2 and Cm-RNS1) under P starvation conditions explained part of the differences in P uptake and PUE among melon accessions. The levels of expression of the other studied genes were diverse among melon accessions, but contributed less to the phenotypical response of the accessions.ConclusionsThis is the first time that these genes have been described in the context of P starvation responses in melon. There exists significant diversity in gene expression levels and P use efficiency among melon accessions as well as significant correlations between gene expression levels and phenotypical measurements.

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Genetics of Root System Architecture Using Near-isogenic Lines of Melon

Cited by 22 publications

References 38 publications

Exploring the Natural Variation for Seedling Traits and Their Link with Seed Dimensions in Tomato

Exploring the Natural Variation for Seedling Traits and Their Link with Seed Dimensions in Tomato

Diversity in root architecture and response to P deficiency in seedlings of Cucumis melo L.

Diversity in Expression of Phosphorus (P) Responsive Genes in Cucumis melo L

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