Diversity in Expression of Phosphorus (P) Responsive Genes in Cucumis melo L

Abstract

BackgroundPhosphorus (P) is a major limiting nutrient for plant growth in many soils. Studies in model species have identified genes involved in plant adaptations to low soil P availability. However, little information is available on the genetic bases of these adaptations in vegetable crops. In this respect, sequence data for melon now makes it possible to identify melon orthologues of candidate P responsive genes, and the expression of these genes can be used to explain the diversity in the root system adaptation to low P availability, recently observed in this species.Methodology and FindingsTranscriptional responses to P starvation were studied in nine diverse melon accessions by comparing the expression of eight candidate genes (Cm-PAP10.1, Cm-PAP10.2, Cm-RNS1, Cm-PPCK1, Cm-transferase, Cm-SQD1, Cm-DGD1 and Cm-SPX2) under P replete and P starved conditions. Differences among melon accessions were observed in response to P starvation, including differences in plant morphology, P uptake, P use efficiency (PUE) and gene expression. All studied genes were up regulated under P starvation conditions. Differences in the expression of genes involved in P mobilization and remobilization (Cm-PAP10.1, Cm-PAP10.2 and Cm-RNS1) under P starvation conditions explained part of the differences in P uptake and PUE among melon accessions. The levels of expression of the other studied genes were diverse among melon accessions, but contributed less to the phenotypical response of the accessions.ConclusionsThis is the first time that these genes have been described in the context of P starvation responses in melon. There exists significant diversity in gene expression levels and P use efficiency among melon accessions as well as significant correlations between gene expression levels and phenotypical measurements.

Transcriptomic Analysis of Banana in Response to Phosphorus Starvation Stress

“…PEPC-malate dehydrogenase-malic enzyme glycolytic bypass, which reduces the requirement for Pi, is a well-documented alteration of glycolysis under Pi starvation [11]. Up-regulation of PEPC and PPCK by P deficiency has been documented in Arabidopsis, rice, white lupin, orange, and melon [12,20,21].…”

Analysis of root traits of watermelon under phosphate fertilization using minirhizotrons

“…The underlying cause for this is the elongation of roots in C1 treatment, a possible strategy of adaptation to low availability of P in the soil (Fita et al, 2012). Research, Society and Development, v. 9, n. 7, e973974560, 2020(CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i7.4560 Meng et al (2014 classified seven genotypes of watermelon with regard to responsiveness and P use efficiency and observed that the capacity of P absorption by the plants correlated with the morphological traits of roots.…”

“…One factor that affects root growth is the availability of phosphorus (P) for plants, to which the latter respond in the form of adaptations in root system morphology and architecture (Fita et al, 2012;Meng et al, 2014) as well as in the partition of assimilates between the roots and the aerial part (Marschner, 2012).…”

Characterization, Identification and Expression Profiling of Genome-Wide R-Genes in Melon and Their Putative Roles in Bacterial Fruit Blotch Resistance

“…Melon (Cucumis melo L.) is a highly diversi ed eudicot diploid (2n = 2x = 24) cucurbitaceous crop with a genome size of approximately 375 Mb [1]. Melon is economically important and ranks as the 9 th most cultivated horticultural crop in terms of worldwide production [2,3]. Its sweet, musky-avored, eshy fruit is rich in vitamins, minerals, and health-promoting antioxidants, including ascorbic acid, carotene, folic acid, and potassium [4][5][6].…”