“…A wild type strain of T. urticae Koch (strain Sambucus) which was collected by HELLE (1962) from elder bushes at Voorne (The Netherlands) in 1961, and the chromosome mutation strain J12 (obtained from the wild strain Baardse) (Overmeer, pers. communication), were cultured in cabinets (size 150 × 50 × 50 era) on bean plants (Phaseolus vulgaris L., variety "Dubbele witte zonder draad") at 23°C, an RH of about 70~ and a daily-light period of about 16 hours.…”
Section: Methodsmentioning
confidence: 99%
“…To gather eggs of a certain age, detached leaf cultures were used (HELLE, 1962). These consisted of a dish of tin-foil (diameter 6 cm) perforated in the bottom and filled with cotton wool, saturated with a sterilized nutrient solution containing per liter of tapwater: 12 gr Ca(NO3)2, 3 gr KH2PO,, 6 gr MgSO,, 5 gr K2SO,.…”
Meiosis in eggs of Tetranychus urticae Koch is described. The two maturation divisions result in (a) a haploid female pronucleus consisting of three karyomeres; -(b) a divided first polar body in which the chromosomes change into karyomeres; -(c) a second polar body, entering a new mitosis which is blocked in metaphase. Irradiation of adult females produced chromosome fragments in the meiotic divisions. The fragments behave as intact chromosomes which proves that during meiosis a diffuse kinetochore is present. The meiotic divisions show the cytologically characteristic features of an inverted meiosis. The presence of such a meiosis is corroborated by observations on eggs heterozygous for chromosome mutations. In both maturation divisions the chromosomes are orientated equatorially. It is suggested that the equatorial orientation is brought about by chiasmata having terminalized to both ends of the dyads. It is argued that in organisms with holokinetic chromosomes during meiosis an axial orientation of the bivalents does not necessarily imply a normal meiosis but can also imply an inverted meiosis.
“…A wild type strain of T. urticae Koch (strain Sambucus) which was collected by HELLE (1962) from elder bushes at Voorne (The Netherlands) in 1961, and the chromosome mutation strain J12 (obtained from the wild strain Baardse) (Overmeer, pers. communication), were cultured in cabinets (size 150 × 50 × 50 era) on bean plants (Phaseolus vulgaris L., variety "Dubbele witte zonder draad") at 23°C, an RH of about 70~ and a daily-light period of about 16 hours.…”
Section: Methodsmentioning
confidence: 99%
“…To gather eggs of a certain age, detached leaf cultures were used (HELLE, 1962). These consisted of a dish of tin-foil (diameter 6 cm) perforated in the bottom and filled with cotton wool, saturated with a sterilized nutrient solution containing per liter of tapwater: 12 gr Ca(NO3)2, 3 gr KH2PO,, 6 gr MgSO,, 5 gr K2SO,.…”
Meiosis in eggs of Tetranychus urticae Koch is described. The two maturation divisions result in (a) a haploid female pronucleus consisting of three karyomeres; -(b) a divided first polar body in which the chromosomes change into karyomeres; -(c) a second polar body, entering a new mitosis which is blocked in metaphase. Irradiation of adult females produced chromosome fragments in the meiotic divisions. The fragments behave as intact chromosomes which proves that during meiosis a diffuse kinetochore is present. The meiotic divisions show the cytologically characteristic features of an inverted meiosis. The presence of such a meiosis is corroborated by observations on eggs heterozygous for chromosome mutations. In both maturation divisions the chromosomes are orientated equatorially. It is suggested that the equatorial orientation is brought about by chiasmata having terminalized to both ends of the dyads. It is argued that in organisms with holokinetic chromosomes during meiosis an axial orientation of the bivalents does not necessarily imply a normal meiosis but can also imply an inverted meiosis.
“…For more detailed descriptions of life-histories, external and internal morphology, and reproductive physiology, reference is made to BLAI~VELT (1945), CAGLE (1949), BOUDREAUX (1963), andHELLE (1962;1967). METCALF & •EWELL (1962), and VEERMAN (pers.…”
Two mutations in Tetranychzts urticae and nine in Tetranychus paci/icus, all originating spontaneously, block the production of red and yellow carotenoid pigments in these spider mite species. Inter-mutant crosses were carried out to study complementation and recombination relationships between the mutations. In T. ,trticae, the two albino mutants complement one another completely, i.e., crosses between them produce wild-type hybrid females; while they recombine with a frequency of 0.05%. Of the nine mutants in T. pacificus, five p mutants in general are complementary to a high degree with four a mutants, p mutants fail to complement one another, while some a mutants are mutually complementary to a slight degree. Scoring the degree of complementation produced by all possible combinations of mutants permits the construction of a linear complemenration map. Certain combinations, however, are exceptional to such a representation. Moreover, marked reciprocal differences in complementation indicate that maternal effects are involved, implying that the albino locus may control more than one enzymatic step. Attempts to derive a genetic map were impeded by the absence of suitable linked markers, by a pronounced maternal effect (high pigmentation) in the haploid F2 males, and by the appearance of "pseudowild" type F2 males. The given genetic sequence, although comparable in a limited fashion to the complementation map, is considered tentative. "Pink" types appeared in crosses with certain p mutants. These were due to mutation at a separate locus, called "rose", and seem to involve the production of pink pigments in an alternative or substitute pathway. A scheme attempting to orientate the present state of understanding of pigmentation in spider mites is presented.
“…In our experiment, photoperiods with 'intermediate' long nights have a higher diapause inductive strength than photoperiods with extreme long nights and than photoperiods near the critical nightlength. Helle (1962) did find in the original 'Voorne' strain of T. urticae a small difference in diapause inductive strength between extreme long nights and intermediate long nights also at a lower temperature. Differences in diapause inductive strength between long-night photoperiods were also found in the bean bug, Riptortus clavatus (Numata, 1985), in the fruitfly, Drosophila testacea, and in the D. auraria species-complex (Kimura, 1982(Kimura, , 1983(Kimura, , 1990.…”
Section: Effect Of Temperature On the Photoperiodic Responsementioning
confidence: 99%
“…Leaves with eggs near hatching were transferred to various photoperiodic regimens at 16°C, 19°C or 22°C, at which the mites completed their entire postembryonic development (below 16°C mites develop very slowly and above 24°C no diapause can be induced; Helle, 1962). The number of females used in each test regimen was about 200.…”
Section: Effect Of Temperature On the Photoperiodic Responsementioning
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.