Genetics of esterases in Drosophila. III. Influence of different chromosomes on esterase pattern in Drosophila

Regulatory aspects of esterase 6 activity variation in sibling Drosophila species

“…simulans-derived EST6 activity (distinguished by its different electrophoretic mobility) is unaffected (Korochkin et a!., 1974;. Similar but smaller effects of the D. simulansderived X chromosome are sometimes seen when a D. melanogaster X chromosome is also present in hybrid females, depending on the parental strains used (Korochkin eta!., 1974).…”

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“…Electrophoresis was performed in Helicon electrophoretic chamber with current rate of 80 mA, voltage of 200 V for 2.5 hours. Histochemical identification of proteins was carried out in accordance with the guidelines (Korochkin et al, 1977;Richardson, 1986). Six enzyme systems were studied: NAD-dependent malate dehydrogenase (MDH, 1.1.1.37), lactate dehydrogenase (LDH, 1.1.1.27), aspartate aminotransferase (AAT, 2.6.1.1), superoxide dismutase (SOD, 1.15.1.1), nonspecific esterases (EST, 3.1.1.1, 3.1.1.2) and myogene system.…”

The expression of esterase S gene of Drosophila virilis in Drosophila melanogaster

“…The estS gene of Drosophila virilis encoding esterase S is an attractive model for studies of gene expression control as this gene is switched on at a well defined period of development (the 3rd day after emergence), and in one tissue (the epithelium of ejaculatory bulbs in males) [1,2]. The enzyme is carboxylic-ester hydrolase; its enzymatic activity is determined by hydrolyzing fl-naphtylacetate [3,4].…”

“…The enzyme is responsible for processes of females fertilization. Esterase S is accumulated in ejaculatory bulbs, excreted and transferred into the genitals of females upon copulation [1][2][3][4][5]. Earlier we described the cloning and some aspects of regulation of estS gene of Drosophila virilis [6][7][8].…”