Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma

Abstract

BackgroundThe Andean cultivar Paloma is resistant to Mesoamerican and Andean races of Colletotrichum lindemuthianum, the fungal pathogen that causes the destructive anthracnose disease in common bean. Remarkably, Paloma is resistant to Mesoamerican races 2047 and 3481, which are among the most virulent races of the anthracnose pathogen. Most genes conferring anthracnose resistance in common bean are overcome by these races. The genetic mapping and the relationship between the resistant Co-Pa gene of Paloma and previously characterized anthracnose resistance genes can be a great contribution for breeding programs.ResultsThe inheritance of resistance studies for Paloma was performed in F2 population from the cross Paloma (resistant) × Cornell 49–242 (susceptible) inoculated with race 2047, and in F2 and F2:3 generations from the cross Paloma (resistant) × PI 207262 (susceptible) inoculated with race 3481. The results of these studies demonstrated that a single dominant gene confers the resistance in Paloma. Allelism tests performed with multiple races of C. lindemuthianum showed that the resistance gene in Paloma, provisionally named Co-Pa, is independent from the anthracnose resistance genes Co-1, Co-2, Co-3, Co-4, Co-5, Co-6, Co-12, Co-13, Co-14, Co-15 and Co-16. Bulk segregant analysis using the SNP chip BARCBean6K_3 positioned the approximate location of Co-Pa in the lower arm of chromosome Pv01. Further mapping analysis located the Co-Pa gene at a 390 kb region of Pv01 flanked by SNP markers SS82 and SS83 at a distance of 1.3 and 2.1 cM, respectively.ConclusionsThe results presented here showed that Paloma cultivar has a new dominant gene conferring resistance to anthracnose, which is independent from those genes previously described. The linkage between the Co-Pa gene and the SS82 and SS83 SNP markers will be extremely important for marker-assisted introgression of the gene into elite cultivars in order to enhance resistance.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3685-7) contains supplementary material, which is available to authorized users.

North-Western Himalayan Common Beans: Population Structure and Mapping of Quantitative Anthracnose Resistance Through Genome Wide Association Study

“…Alternatively, our material showed anthracnose resistance to race 2047 and resistance can easily be transferred to local commercially grown cultivars because these cultivars are adapted to the local environment and do not have limitations as those of G2333 and other differential cultivars. Over past few years, new anthracnose resistance genes were identified and mapped from diverse anthracnose resistant genotypes ( Gonçalves-Vidigal et al, 2011 ; Richard et al, 2014 ; Lacanallo and Gonçalves-Vidigal, 2015 ; Trabanco et al, 2015 ; Chen et al, 2017 ; de Lima Castro et al, 2017 ). We expect our anthracnose resistant common bean material may contain diverse anthracnose resistance genes as the C. lindemuthianum virulence spectrum in NW region is different than the US, France and Canada ( Padder et al, 2017 ).…”

“…Seventeen of these genes are numbered from Co-1 to Co-17 , however many anthracnose resistance genes have alphabets as suffix such as Co-x , Co-y , Co-w , Co-Pa , Co-z , Co-1 HY , and are mapped to bean chromosomes where numbered genes are located ( Zuiderveen et al, 2016 ). Co-1 and its alleles, Co-14 , Co-x , Co-w , Co-1 HY , Co-1 65-X , Co-1 73-X , and Co-Pa anthracnose resistance genes are mapped to the distal end of bean chromosome Pv01 ( Melotto and Kelly, 2000 ; Geffroy et al, 2008 ; Gonçalves-Vidigal et al, 2011 ; Campa et al, 2014 ; Richard et al, 2014 ; Chen et al, 2017 ; de Lima Castro et al, 2017 ). Five anthracnose resistance genes ( Co-u , CoPv02c 3-X , CoPv02c 7-X , CoPv02c 19-X , and CoPv0c 2449-X ) are mapped to Pv02 ( Kelly et al, 2003 ; Campa et al, 2014 ).…”

“…Genome wide association studies (GWAS) showed association of a SNP and SSR marker located at Pv10 with anthracnose resistance ( Zuiderveen et al, 2016 ; Wu et al, 2017 ). Access to P. vulgaris whole genome ( Schmutz et al, 2014 ; Vlasova et al, 2016 ) and SNP Bean Chips ( Hyten et al, 2010 ; Song et al, 2015 ) has resulted in the fine mapping of many economically important traits in beans including anthracnose resistance ( Richard et al, 2014 ; Burt et al, 2015 ; Gonzalez et al, 2015 ; Oblessuc et al, 2015 ; Padder et al, 2016 ; Zuiderveen et al, 2016 ; Chen et al, 2017 ; de Lima Castro et al, 2017 ; Mungalu et al, 2020 ).…”

Role of phospholipase C in banana in response to anthracnose infection

“…PLC is an enzyme that hydrolyzes plasma membrane phospholipids at the ester bond of the third position of the glycerol backbone (Fain, ). The PLC family comprises multiple members in plants (Canonne, Froidure‐Nicolas, & Rivas, ), and PLCs have been reported to play an important role in plant immunity (de Lima Castro et al, ; Tsai & Chung, ), depending on the types of pathogens and specific PLCs (Legendre et al, ; van der Luit et al, ). A previous study demonstrated that three phosphoinositide‐specific PLCs were mapped by the anthracnose resistance gene (Richard et al, ).…”

Identification of race‐specific quantitative trait loci for resistance to Colletotrichum lindemuthianum in an Andean population of common bean

“…Middle American genes include Co‐2 , Co‐3 , Co‐4 , Co‐5 , Co‐6 , Co‐11 , Co‐16 , Co‐17 , Co‐u , and Co‐v on chromosomes Pv02, Pv03, Pv04, Pv07, Pv08, and Pv11 (Bannerot, Derieux, & Fouilloux, 1971; Coelho et al., 2013; Coimbra‐Gonçalves et al., 2016; Fouilloux, 1976; Geffroy, 1997; Geffroy, Sévignac, Billant, Dron, & Langin, 2008; Goncalves‐Vidigal, Cardoso, Vieira, & Saralva, 1997; Gonçalves‐Vidigal, Silva, Vidigal Filho, Gonela, & Kvitschal, 2007; Mastenbroek, 1960; Trabanco, Campa, & Ferreira, 2015; Young & Kelly, 1996; Young, Melotto, Nodari, & Kelly, 1998). Andean genes are Co‐1 , Co‐12 , Co‐13 , Co‐14 , Co‐15 , Co‐x , Co‐w , Co‐y , Co‐z , Co‐Pa , and Co‐AC on chromosomes Pv01, Pv03, and Pv04 (Cardenas, Adams, & Andersen, 1964; de Lima Castro et al., 2017; Geffroy et al., 1999; Geffroy et al., 2008; Gilio et al., 2017; Gonçalves‐Vidigal & Kelly, 2006; Gonçalves‐Vidigal, Lacanallo, & Vidigal, 2008; Gonçalves‐Vidigal et al., 2012; Gonçalves‐Vidigal, Vidigal Filho, Medeiros, & Pastor‐Corrales, 2009; Gonçalves‐Vidigal et al., 2016; Lacanallo & Gonçalves‐Vidigal, 2015; Melotto & Kelly, 2000; Sousa et al., 2015). The genes Co‐1 , Co‐3 , Co‐4 , and Co‐5 are multi‐allelic (Fouilloux, 1976; Melotto & Kelly, 2000; Young & Kelly, 1996; Young et al., 1998).…”