Genetics and Biochemistry of Seed Flavonoids

Lepiniec, Loïc; Debeaujon, Isabelle; Routaboul, Jean‐Marc; Baudry, Antoine; Pourcel, Lucille; Nési, Nathalie; Caboche, Michel

doi:10.1146/annurev.arplant.57.032905.105252

Cited by 1,059 publications

(1,080 citation statements)

References 160 publications

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“…Stracke et al (2007) found that AtMYB11/PFG1, AtMYB12/PFG1 and AtMYB111/PFG3 controled flavones biosynthesis in all tissues while AtMYB75/ PAP1, AtMYB90/PAP2, AtMYB113 and AtMYB114 regulated anthocyanin biosynthesis in vegetative tissues (Gonzalez et al 2008) while AtMYB123/TT2 controlled the biosynthesis of proanthocyanidins (PAs) in the seed coat of Arabidopsis (Lepiniec et al 2006). Verdier et al (2012).…”

Section: Regulation Of Primary and Secondary Metabolismmentioning

confidence: 99%

MYB transcription factor genes as regulators for plant responses: an overview

Ambawat

Sharma

Yadav

et al. 2013

Physiol Mol Biol Plants

783

544

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Regulation of gene expression at the level of transcription controls many crucial biological processes. Transcription factors (TFs) play a great role in controlling cellular processes and MYB TF family is large and involved in controlling various processes like responses to biotic and abiotic stresses, development, differentiation, metabolism, defense etc. Here, we review MYB TFs with particular emphasis on their role in controlling different biological processes. This will provide valuable insights in understanding regulatory networks and associated functions to develop strategies for crop improvement.

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Section: Regulation Of Primary and Secondary Metabolismmentioning

confidence: 99%

MYB transcription factor genes as regulators for plant responses: an overview

Ambawat

Sharma

Yadav

et al. 2013

Physiol Mol Biol Plants

783

544

View full text Add to dashboard Cite

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“…Because fie and msi1 mutants are seed lethal (Ohad et al, 1999;Hennig et al, 2003;Köhler et al, 2003), we studied heterozygous plants. Emasculated fie/+ and msi1/+ flowers produce both wild-type-looking ovules ( Figure 3A) and enlarged autonomous seeds having a partially developed seed coat that accumulates proanthocyanidins (PAs) (Figures 3B and 3D) (Roszak and Köhler, 2011), which are flavonoid compounds responsible for the characteristic brown color of Arabidopsis seeds (Supplemental Figure 4) (Lepiniec et al, 2006) . We counted the number of nucellus cells in the central longitudinal section of unfertilized fie/+ and msi1/+ ovules and enlarged autonomous seeds.…”

Section: Polycomb Proteins Repress the Degeneration Of The Nucellusmentioning

confidence: 99%

“…TT16 promotes the differentiation of the seed endothelium, the innermost layer of the seed coat. tt16 mutant seeds display abnormally shaped endothelium cells and fail to synthetize PAs (Nesi et al, 2002;Lepiniec et al, 2006). We analyzed the central longitudinal section of tt16 mutant ovules and seeds between 0 and 8 DAF ( Figures 1F to 1J).…”

Section: Tt16 Promotes Nucellus Degenerationmentioning

confidence: 99%

Endosperm and Nucellus Develop Antagonistically in Arabidopsis Seeds

et al. 2016

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In angiosperms, seed architecture is shaped by the coordinated development of three genetically different components: embryo, endosperm, and maternal tissues. The relative contribution of these tissues to seed mass and nutrient storage varies considerably among species. The development of embryo, endosperm, or nucellus maternal tissue as primary storage compartments defines three main typologies of seed architecture. It is still debated whether the ancestral angiosperm seed accumulated nutrients in the endosperm or the nucellus. During evolution, plants shifted repeatedly between these two storage strategies through molecular mechanisms that are largely unknown. Here, we characterize the regulatory pathway underlying nucellus and endosperm tissue partitioning in Arabidopsis thaliana. We show that Polycomb-group proteins repress nucellus degeneration before fertilization. A signal initiated in the endosperm by the AGAMOUS-LIKE62 MADS box transcription factor relieves this Polycomb-mediated repression and therefore allows nucellus degeneration. Further downstream in the pathway, the TRANSPARENT TESTA16 (TT16) and GORDITA MADS box transcription factors promote nucellus degeneration. Moreover, we demonstrate that TT16 mediates the crosstalk between nucellus and seed coat maternal tissues. Finally, we characterize the nucellus cell death program and its feedback role in timing endosperm development. Altogether, our data reveal the antagonistic development of nucellus and endosperm, in coordination with seed coat differentiation.

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“…Thirty-five flavonoid biosynthetic genes, including genes encoding 12 transcription factors, 10 structural enzymes, and 10 modification enzymes, have been identified (Tohge et al, 2005Lepiniec et al, 2006;Fraser et al, 2007;Luo et al, 2007;Stracke et al, 2007;Yonekura-Sakakibara et al, 2007), but even in this highly studied species, the full details of the flavonoid pathway, including modification, are still unknown. The major flavonoid structures in Arabidopsis suggest that there are as yet unidentified modification enzyme genes and pathways in flavonoid metabolism.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Flavonol Profiling and Transcriptome Coexpression Analysis Leading to Decoding Gene–Metabolite Correlations inArabidopsis

et al. 2008

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To complete the metabolic map for an entire class of compounds, it is essential to identify gene-metabolite correlations of a metabolic pathway. We used liquid chromatography-mass spectrometry (LC-MS) to identify the flavonoids produced by Arabidopsis thaliana wild-type and flavonoid biosynthetic mutant lines. The structures of 15 newly identified and eight known flavonols were deduced by LC-MS profiling of these mutants. Candidate genes presumably involved in the flavonoid pathway were delimited by transcriptome coexpression network analysis using public databases, leading to the detailed analysis of two flavonoid pathway genes, UGT78D3 (At5g17030) and RHM1 (At1g78570). The levels of flavonol 3-Oarabinosides were reduced in ugt78d3 knockdown mutants, suggesting that UGT78D3 is a flavonol arabinosyltransferase. Recombinant UGT78D3 protein could convert quercetin to quercetin 3-O-arabinoside. The strict substrate specificity of UGT78D3 for flavonol aglycones and UDP-arabinose indicate that UGT78D3 is a flavonol arabinosyltransferase. A comparison of flavonol profile in RHM knockout mutants indicated that RHM1 plays a major role in supplying UDPrhamnose for flavonol modification. The rate of flavonol 3-O-glycosylation is more affected than those of 7-O-glycosylation by the supply of UDP-rhamnose. The precise identification of flavonoids in conjunction with transcriptomics thus led to the identification of a gene function and a more complete understanding of a plant metabolic network.

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Genetics and Biochemistry of Seed Flavonoids

Cited by 1,059 publications

References 160 publications

MYB transcription factor genes as regulators for plant responses: an overview

MYB transcription factor genes as regulators for plant responses: an overview

Endosperm and Nucellus Develop Antagonistically in Arabidopsis Seeds

Comprehensive Flavonol Profiling and Transcriptome Coexpression Analysis Leading to Decoding Gene–Metabolite Correlations inArabidopsis

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