Genetically stable plants with boosted flavonoids content after in vitro regeneration of the endangered Capparis spinosa L

Goda, Marwa S.; Sá, A.J.; F, El Sherif; Hassanean, HA; Ak, Ibrahim

doi:10.15761/gdt.1000124

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…Another direction of the study can be dealt with the selection of clones with high flavonoid content. Our results are in accordance with the reports on high flavonoid content of micropropagated plants (Rad et al, 2014;Goda et al, 2017), but the level can be increased.…”

Section: Discussionsupporting

confidence: 93%

Flavonoids and Antimicrobial Properties of Begonia fischeri var. palustris in vitro Plantlets

Karpova¹,

Nabieva²,

Fershalova³

et al. 2019

OnLine Journal of Biological Sciences

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The objective of this study was to design a protocol of the successful establishment of plants of Begonia fischeri var. palustris obtained in the in vitro seeds culture, to evaluate flavonoid content and antimicrobial properties of in vitro plantlets. The significant increase of percentage of seed germination (92.5%) was recorded in the half strength MS medium and 1% agar medium (83.3%) in comparison with 50-60% in greenhouse conditions. Flavonoid composition of the leaves of in vitro plantlets and greenhouse stock plants had no substantial differences. Significant differences (p<0.05) were not found between flavonoid contents of the leaves (13.6 and 15.5 mg•g −1 of Dry Weight (DW), respectively). Aqueous ethanol extracts of plants showed antimicrobial effects against reference strains of Bacillus subtilis, Streptococcus pyogenes and Staphylococcus aureus. Concentration of flavonoids in acetone and ethanol extracts of exudative compounds of the leaves of in vitro plantlets was 0.02 and 2.0 mg•g −1 DW, respectively. Our results indicated that this protocol can be used for the production of a large number of plants of B. fischeri var. palustris with rich flavonoid composition and high quantities of flavonoids, including exudative compounds, with healing and air cleaning properties.

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Section: Discussionsupporting

confidence: 93%

Flavonoids and Antimicrobial Properties of Begonia fischeri var. palustris in vitro Plantlets

Karpova¹,

Nabieva²,

Fershalova³

et al. 2019

OnLine Journal of Biological Sciences

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show abstract

“…In this study, the total avonoid in the 2 mg L NAA + 1 mg/L BAP + 0.1 µM (P < 0.05) treatment increased by 19% when compared to the control. Goda et al (2017) found that the total avonoid content in C. spinosa callus from the 2,4-D medium was 0.85 mg/100 mg. It has also been stated in many studies that the rutin contained in the aerial parts were dominant avonoid (Zhou et al 2011;Argentieri et al 2012).…”

Section: Resultsmentioning

confidence: 96%

Evaluating The Impact of Strigolactone GR24 On Capparis Spinosa L. Callus Production And Phenolic Compound Content

Issah

Duran

2021

Preprint

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The effect of strigolactones on plants, which has been recently described as a new group of plant hormones, has not been fully characterized. Capparis spinosa L. callus formation using synthetic strigolactone GR24 (0.1 and 0.2 μM) alone or in combination with 1-naphthalene acetic acid (NAA) (2 mg / L) and 6-benzylaminopurine (BAP) (1 mg / L) and its effect on phenolic substance production were evaluated. 2 mg/L NAA+1 mg/L BAP+0.1 μM GR24 was the medium with the highest callus formation (60.3%) and callus fresh weight (120.8 mg). In the phytochemical analysis, the highest total flavonoid and phenolic substance and the highest rutin, quercetin, chlorogenic acid content were found in this application and their amounts increased at various rates compared to the control. Aromatic substances in caper calluses were grouped as sulfur compounds (66.97% -87.53%), aldehydes (4.88% -7.90%), ketones (0.34% -19.3%), hydrocarbons and derivatives (0.56%-5.8%), alcohols (% 1.62-6.08%), others (0.61% -2.37%) and their amounts varied at various hormone applications. When 0.1 μM GR24 was applied alone, the total sulfur compound in callus samples was 87.53% and the dominant substance was found to be methyl isothiocyanate.

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The impact of strigolactone GR24 on Capparis spinosa L. callus production and phenolic compound content

Duran

Issah

2022

Plant Cell Tiss Organ Cult

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Genetically stable plants with boosted flavonoids content after in vitro regeneration of the endangered Capparis spinosa L

Cited by 5 publications

References 16 publications

Flavonoids and Antimicrobial Properties of Begonia fischeri var. palustris in vitro Plantlets

Flavonoids and Antimicrobial Properties of Begonia fischeri var. palustris in vitro Plantlets

Evaluating The Impact of Strigolactone GR24 On Capparis Spinosa L. Callus Production And Phenolic Compound Content

The impact of strigolactone GR24 on Capparis spinosa L. callus production and phenolic compound content

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