Flavonoids and Antimicrobial Properties of Begonia fischeri var. palustris in vitro Plantlets

Abstract

The objective of this study was to design a protocol of the successful establishment of plants of Begonia fischeri var. palustris obtained in the in vitro seeds culture, to evaluate flavonoid content and antimicrobial properties of in vitro plantlets. The significant increase of percentage of seed germination (92.5%) was recorded in the half strength MS medium and 1% agar medium (83.3%) in comparison with 50-60% in greenhouse conditions. Flavonoid composition of the leaves of in vitro plantlets and greenhouse stock plants had no substantial differences. Significant differences (p<0.05) were not found between flavonoid contents of the leaves (13.6 and 15.5 mg•g −1 of Dry Weight (DW), respectively). Aqueous ethanol extracts of plants showed antimicrobial effects against reference strains of Bacillus subtilis, Streptococcus pyogenes and Staphylococcus aureus. Concentration of flavonoids in acetone and ethanol extracts of exudative compounds of the leaves of in vitro plantlets was 0.02 and 2.0 mg•g −1 DW, respectively. Our results indicated that this protocol can be used for the production of a large number of plants of B. fischeri var. palustris with rich flavonoid composition and high quantities of flavonoids, including exudative compounds, with healing and air cleaning properties.

In Vitro Screening for Antimicrobial Potential of Ethanolic Leaf Extracts of Some Begonia Species Against Methicillin-Resistant Staphylococcus Aureus (Mrsa) Strain

“…The contents of anthocyanins were between 60 and 157 mg%, ascorbic acid -5-43 mg% of fresh weight. These results suggest that studied plants of Begonia species can be considered as the sources of biologically active compounds with promising antioxidant and antimicrobial activities [12,13].…”

“…Staphylococcus epidermidis, Escherichia coli, and Candida albicans). As a result, 14 Begonia species, possessing well-expressed phytoncide activity have been recommended to use as indoor plants, based on their ability to reduce microbial air pollution indoor by a factor of 1,5-3,0 in particular, by decreasing the Staphylococcus aureus load [12,13,25].…”

Screening for Antimicrobial Activity of Nine Ethanolic Extracts Obtained from Leaves of Begonia Plant: a Possible Alternative in the Treatment of Infections Caused by Citrobacter Freundii

“…Antimicrobial properties of Begonia fischeri var. palustris plantlets were assessed by Karpova et al (2019). Flavonoid composition of the leaves of in vitro plantlets and greenhouse stock plants had no substantial differences.…”

“…Aqueous ethanolic extracts of plants showed antimicrobial effects against reference strains of Bacillus subtilis, Streptococcus pyogenes and Staphylococcus aureus. The concentration of flavonoids in acetone and ethanol extracts of exudative compounds of the leaves of in vitro plantlets was 0.02 and 2.0 mg/g DW, respectively(Karpova et al, 2019). Streptococcus pyogenes) by the disc diffusion method.…”

Seasonal antimicrobial activity of volatile substances emitted by the representatives of <i>Begonia</i> L. genus (Begoniaceae)

“…Т. 10, № 1 виды и культивары, расширить ассортимент для озеленения интерьеров различного назначения [1].…”