Genetically modified plants and food hypersensitivity diseases: Usage and implications of experimental models for risk assessment

Safe use of Cry genes in genetically modified crops

“…For example, interaction of the transgene conferring regulatory proteins if transferred into an entirely different background may fluctuate (De Schrijver et al 2007). At this point of time, sequence analysis of amino acids is difficult to predict, thus limiting their utility for comparing their sequences with the known allergens (Prescott and Hogan 2006). Also quantifying their degradation in vitro system has been the major challenge in establishing valid correlations with allergens (Bannon et al 2003) which set a stage for conducting such experiments in vivo systems (Pusztai et al 2003).…”

Biosafety Risk of Genetically Modified Crops Containing Cry Genes

“…For example, interaction of the transgene conferring regulatory proteins if transferred into an entirely different background may change (De Schrijver et al 2007). At this point of time, sequence analysis of amino acids is difficult to predict, thus limiting their utility for comparing their sequences with the known allergens (Alinorm 2003;Prescott and Hogan 2005). Also quantifying their degradation in vitro system has been the major challenge in establishing valid correlations with allergens (Bannon et al 2003) which set a stage for conducting such experiments in vivo systems (Pusztai et al 2003).…”

Systematic analysis of histamine and N-methylhistamine concentrations in organs from two common laboratory mouse strains: C57Bl/6 and Balb/c

“…The cause for this reaction could be an unidentified infection, which, however, must be a very mild one, since the gross pathology of the mice was normal. However, the preferred use of Balb/c mice in allergic models, including those for food allergies, correlates well with the increased concentration of histamine in the intestine [28,29].…”