Genetically modified microorganisms as producers of biologically active compounds

Background. A selective system based on the M1 virus of the yeast Saccharomyces cerevisiae was proposed. Methods. To create a recipient strain, a DNA fragment encoding the killer toxin of the M1 virus under the control of the regulated promoter of the GAL1 gene was inserted into the genome of S. cerevisiae strains Y-1236 and Y-2177. Results. Integration of such expression cassette leads to the conditional lethality - resulting strains die on a medium with galactose when killer toxin synthesis occurs. A linear DNA fragment containing the gene of interest flanked by sequences homologous to the promoter of the GAL1 gene and the termination region of the CYC1 gene is used to transform the obtained strains. During transformation due to homologous recombination, the sequence encoding the killer toxin is cleaved and the transformants grow on a medium with galactose. Conclusion. The proposed selective system combines the main advantages of other systems: the use of simple media, without the need to add expensive antibiotics, and a simplified technique for constructing expression cassettes and selecting transformants.

show abstract

Application of Thermostable Lipolytic Bacterial Enzymes for Modern Biotechnological Processes: Review

Самойлова

Sorokina

Piligaev

et al. 2018

Kat.v.prom.

View full text Add to dashboard Cite

In the review paper, the modern investigations on the application of thermostable lipolytic bacterial enzymes for biotechnology are discussed, the properties of these enzymes discussed including their activity and functional stability at various temperatures, pH in organic solvents, as well as the substrate specificity and activity in the presence of various chemical compounds. The paper contains data on the development of recombinant producers of lipolytic bacterial enzymes and on approaches to improving their productivity. The application of the bacterial lipases for biotechnological processes of synthesis of biofuel, various chemicals and detergents, for food industry and wastewater treatment is considered.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Genetically modified microorganisms as producers of biologically active compounds

Abstract: In the review the data on use of genetically modified microorganisms as producers of proteins of different organisms are presented. The relative advantages and disadvantages of bacterial and yeast systems for heterologous genes expression are considered.

Cited by 3 publications

References 123 publications

Application of Bacterial Thermostable Lipolytic Enzymes in the Modern Biotechnological Processes: A Review

Application of Bacterial Thermostable Lipolytic Enzymes in the Modern Biotechnological Processes: A Review

Selective system based on fragments of the M1 virus for the yeast Saccharomyces cerevisiae transformation

Application of Thermostable Lipolytic Bacterial Enzymes for Modern Biotechnological Processes: Review

Contact Info

Product

Resources

About