Genetically anchored fluorescent probes for subcellular specific imaging of hydrogen sulfide

Abstract

Imaging hydrogen sulfide (H2S) at the subcellular resolution will greatly improve the understanding of functions of this signaling molecule. Taking advantage of the protein labeling technologies, we report a general strategy for the development of organelle specific H2S probes, which enables sub-cellular H2S imaging essentially in any organelles of interest.

Organelle-Targeted H2S Probes Enable Visualization of the Subcellular Distribution of H2S Donors

“…We note that initial applications of this approach for H 2 S sensing appeared in the literature during the review process of the present manuscript. 16 Two of the most popular of such techniques include HaloTag, which uses alkyl chlorides in combination with dehalogenase enzymes, and SNAP-tag, which uses benzylguanine-ligated substrates in combination with AGT fusion proteins to generate subcellularly localized probes. 17–19 Motivated by this need, we report here the development of a genetically registered, organelle-targeted H 2 S probe utilizing SNAP-tag methodology and use this developed system to image the differential subcellular donation of H 2 S from selected synthetic donors.…”

Dual‐Functionalisation of Fluorophores for the Preparation of Targeted and Selective Probes

“…CouN 3 -BC ( Figure 2b)i satargeted, reaction-based H 2 S probe that relies on the hydrogen sulfide mediated reduction of azides to their corresponding amines. [36] Theazide sensing group,w hich quenches coumarin fluorescence,w as installed through diazotisation of 7-aminocoumarin-3-carboyxlic acid. Reduction restores the fluorescent 7-aminocoumarin (l ex = 405 nm, l em = 450 nm).…”

Duale Funktionalisierung von Fluorophoren für die Konstruktion zielgerichteter und selektiver Fluoreszenz‐Sensoren

“…Bis dato sind nur wenige dual-funktionali-sierte Cumarine beschrieben, welche sich gezielt lokalisieren und biologische Analyte nachweisen, und alle bisher beschriebenen Beispiele basieren auf Cumarinen, welche an der C3-und C7-Position funktionalisiert sind. [36][37] Spezifische Beispiele werden im Folgenden beschrieben.…”