Duale Funktionalisierung von Fluorophoren für die Konstruktion zielgerichteter und selektiver Fluoreszenz‐Sensoren

Trinh, Natalie; Jolliffe, Katrina A.; New, Elizabeth J.

doi:10.1002/ange.202007673

Angewandte Chemie

2020

DOI: 10.1002/ange.202007673

|View full text |Cite

Duale Funktionalisierung von Fluorophoren für die Konstruktion zielgerichteter und selektiver Fluoreszenz‐Sensoren

Natalie Trinh¹,

Katrina A. Jolliffe

Elizabeth J. New

Abstract: Abbildung 4. (a) BODIPY-Struktur mit Nummerierung (b) Strukturen von BODIPY-basierenden dual-funktionalisiertenS ensoren.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2021

2023

Publication Types

Select...

Article5

Relationship

Self Cite0

Independent5

Authors

Journals

Cited by 11 publications

References 110 publications

(208 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Shadow Electrochemiluminescence Microscopy of Single Mitochondria

Colin

Descamps

et al. 2021

Angewandte Chemie

View full text Add to dashboard Cite

Mitochondria are the subcellular bioenergetic organelles. The analysis of their morphology and topology is essential to provide useful information on their activity and metabolism. Herein, we report a label‐free shadow electrochemiluminescence (ECL) microscopy based on the spatial confinement of the ECL‐emitting reactive layer to image single living mitochondria deposited on the electrode surface. The ECL mechanism of the freely‐diffusing [Ru(bpy)3]2+ dye with the sacrificial tri‐n‐propylamine coreactant restrains the light‐emitting region to a micrometric thickness allowing to visualize individual mitochondria with a remarkable sharp negative optical contrast. The imaging approach named “shadow ECL” (SECL) reflects the negative imprint of the local diffusional hindrance of the ECL reagents by each mitochondrion. The statistical analysis of the colocalization of the shadow ECL spots with the functional mitochondria revealed by classical fluorescent biomarkers, MitoTracker Deep Red and the endogenous intramitochondrial NADH, validates the reported methodology. The versatility and extreme sensitivity of the approach are further demonstrated by visualizing single mitochondria, which remain hardly detectable with the usual biomarkers. Finally, by alleviating problems of photobleaching and phototoxicity associated with conventional microscopy methods, SECL microscopy should find promising applications in the imaging of subcellular structures.

show abstract

Shadow Electrochemiluminescence Microscopy of Single Mitochondria

Colin

Descamps

et al. 2021

Angewandte Chemie

View full text Add to dashboard Cite

show abstract

Photocleavable Fluorescent Membrane Tension Probes: Fast Release with Spatiotemporal Control in Inner Leaflets of Plasma Membrane, Nuclear Envelope, and Secretory Pathway

et al. 2021

View full text Add to dashboard Cite

Mechanosensitive flipper probes are attracting interest as fluorescent reporters of membrane order and tension in biological systems. We introduce PhotoFlippers, which contain a photocleavable linker and an ultralong tether between mechanophore and various targeting motifs. Upon irradiation, the original probe is released and labels the most ordered membrane that is accessible by intermembrane transfer. Spatiotemporal control from photocleavable flippers is essential to access open, dynamic or elusive membrane motifs without chemical or physical interference. For instance, fast release with light is shown to place the original small‐molecule probes into the innermost leaflet of the nuclear envelope to image changes in membrane tension, at specific points in time of membrane trafficking along the secretory pathway, or in the inner leaflet of the plasma membrane to explore membrane asymmetry. These results identify PhotoFlippers as useful chemistry tools to enable research in biology.

show abstract

Dual‐Color Photoconvertible Fluorescent Probes Based on Directed Photooxidation Induced Conversion for Bioimaging

et al. 2022

View full text Add to dashboard Cite

We herein present a new concept to produce dual-color photoconvertible probes based on a mechanism called Directed Photooxidation Induced Conversion (DPIC). As a support of this mechanism, styrylcoumarins (SCs) bearing Aromatic Singlet Oxygen Reactive Moieties (ASORMs) like furan and pyrrole have been synthesized. SCs are bright fluorophores, which undergo a hypsochromic conversion upon visible light irradiation due to directed photooxidation of the ASORM that leads to the disruption of conjugation. SC-P, a yellow emitting probe bearing a pyrrole moiety, converts to a stable blue emitting coumarin with a 68 nm shift allowing the photoconversion and tracking of lipid droplet in live cells. This new approach might pave the way to a new generation of photoconvertible dyes for advanced bioimaging applications.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Duale Funktionalisierung von Fluorophoren für die Konstruktion zielgerichteter und selektiver Fluoreszenz‐Sensoren

Abstract: Abbildung 4. (a) BODIPY-Struktur mit Nummerierung (b) Strukturen von BODIPY-basierenden dual-funktionalisiertenS ensoren.

Cited by 11 publications

References 110 publications

Shadow Electrochemiluminescence Microscopy of Single Mitochondria

Shadow Electrochemiluminescence Microscopy of Single Mitochondria

Photocleavable Fluorescent Membrane Tension Probes: Fast Release with Spatiotemporal Control in Inner Leaflets of Plasma Membrane, Nuclear Envelope, and Secretory Pathway

Dual‐Color Photoconvertible Fluorescent Probes Based on Directed Photooxidation Induced Conversion for Bioimaging

Contact Info

Product

Resources

About