Genetical and Biochemical Studies of Pteridine Granule Membrane in Larval Hypodermal Cells of the Silkworm

Sakurai, Susumu; Tsujita, Mitsuo

doi:10.1266/jjg.51.39

Cited by 6 publications

(1 citation statement)

References 20 publications

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“…During early stages of the fifth instar, ribosomes were attached to the granule membrane but decreased to zero on the 5th day of the instar (15). Sakurai paid special attention to the granule membrane and described properties of the membrane protein and considered that the ribosomes attached to the membrane may be concerned with the biosynthesis of the membrane protein (16).…”

Section: Introductionmentioning

confidence: 99%

Localization of Sepiapterin Deaminase and Pteridines In the Granules In Epidermal Cells of the Silkworm, Bombyx mori

1990

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SummaryA new method for assay of sepiapterin deaminase (3.5.4.24) activity by use of high performance liquid chromatography (HPLC) was developed. By this sensitive method the enzyme activity in the cell organelle was assayed. After cell fractionation, the enzyme was extracted with deoxycholate from pteridine granules of epidermal cells of the lemon mutant silkworm. On stepwise sucrose gradient centrifugation, most of the enzyme activity localized in the aggregated pteridine granules fraction, while the soluble fraction contained only one fourth of the total enzyme activity. The enzyme in the granule fraction had the same properties as the previously reported sepiapterin deaminase. These data show that the enzyme is localized in pteridine granules in the living cells. The attachment of the enzyme to the granule membrane is rather loose and previous papers studied the enzyme released from the granules.Cell fractionation and morphological observation showed that sepiapterin, sepialumazine and other pteridines were also localized in the granules together with uric acid.

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Section: Introductionmentioning

confidence: 99%