Genetical and biochemical studies of yellow lethal larvae in the silkworm

Tsujita, Mitsuo; Sakaguchi, Bungo

doi:10.1266/jjg.30.83

Cited by 6 publications

(3 citation statements)

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“…Previous studies showed that yellow pigments in the lem larvae contain SP, sepialumazine (deaminized product of SP), and riboflavin (Tsusue et al 1965), of which SP is quantitatively major component accumulated in the integument (Tsujita et al1955, 1961). Accumulation of SP in large amounts is due to a lack of the enzyme SP reductase in the mutant strain (Matsubara et al 1963; Toshio et al 1980).…”

Section: Introductionmentioning

confidence: 99%

“…The lemon ( lem ) mutant is a body color mutant of the silkworm, Bombyx mori (L.) (Lepidoptera: Bombycidae), and displays yellow body coloration during larval developmental stages ( Figure 1A ), which is markedly different from body coloration of wild-type strains ( Figure 1B ). Previous studies showed that yellow pigments in the lem larvae contain SP, sepialumazine (deaminized product of SP), and riboflavin ( Tsusue et al 1965 ), of which SP is quantitatively major component accumulated in the integument ( Tsujita et al1955 , 1961 ). Accumulation of SP in large amounts is due to a lack of the enzyme SP reductase in the mutant strain ( Matsubara et al 1963 ; Toshio et al 1980 ).…”

Section: Introductionmentioning

confidence: 99%

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Isolation, Purification, and Identification of an Important Pigment, Sepiapterin, from Integument of thelemonMutant of the Silkworm,Bombyx mori

Gao

Wang

et al. 2013

Journal of Insect Science

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Sepiapterin is the precursor of tetrahydrobiopterin, an important coenzyme of aromatic amino acid hydroxylases, the lack of which leads to a variety of physiological metabolic diseases or neurological syndromes in humans. Sepiapterin is a main pigment component in the integument of the lemon mutant of the silkworm, Bombyx mori (L.) (Lepidoptera: Bombycidae), and is present there in extremely high content, so lemon is a valuable genetic resource to extract sepiapterin. In this study, an effective experimental system was set up for isolation and purification of sepiapterin from lemon silkworms by optimizing homogenization solvent, elution buffer, and separation chromatographic column. The results showed that ethanol was the most suitable solvent to homogenize the integument, with a concentration of 50% and solid:liquid ratio of 1:20 (g/mL). Sepiapterin was purified successively by column chromatography of cellulose Ecteola, sephadex G-25-150, and cellulose phosphate, and was identified by ultraviolet-visible absorption spectrometry. A stable and accurate high performance liquid chromatography method was constructed to identify sepiapterin and conduct qualitative and quantitative analyses. Sepiapterin of high purity was achieved, and the harvest reached about 40 ug/g of integument in the experiments. This work helps to obtaining natural sepiapterin in large amounts in order to use the lemon B. mori mutant to produce BH4 in vitro.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation, Purification, and Identification of an Important Pigment, Sepiapterin, from Integument of thelemonMutant of the Silkworm,Bombyx mori

Gao

Wang

et al. 2013

Journal of Insect Science

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“…Tsujita and Sakaguchi (1955) showed an allelomorphism in the lemon lethal, lemon and normal strains by genetic analysis of the Fl hybrid between the homozygous lemon (lem Jlem) and the heterozygous lethal yellow (+ lleml). A dominant recessive relation + lem > lem>lem' was also reported.…”

Section: Introductionmentioning

confidence: 99%

Studies on the genetical and biochemical properties of the Kiuki mutant of the silkworm, Bombyx mori.

Mazda

Tsusué

Sakate

et al. 1981

The Japanese journal of genetics

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Genetic studies of the Bombyx mori Kiuki mutant were made on the segregation of the F2 and BFl phenotypes. Assay of sepialumazine in these phenotypes revealed that the Kiuki mutant is an incomplete dominant. A symbol Sel is given to the mutant gene. Enzymatic studies on F2 progeny showed that the yellow larval integument colour characteristic of the mutant, which is due to accumulation of sepialumazine, is dependent on the activity of sepiapterin deaminase. Linkage test showed that the Sel is independent of the so far established 23 linkage groups but represents one of the still unknown group among remaining five chromosomes.

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Pterine als Wirkstoffe und Pigmente

Ziegler

1965

Ergebnisse der Physiologie und exper. Pharmakologie

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Genetical and biochemical studies of yellow lethal larvae in the silkworm

Cited by 6 publications

References 0 publications

Isolation, Purification, and Identification of an Important Pigment, Sepiapterin, from Integument of thelemonMutant of the Silkworm,Bombyx mori

Isolation, Purification, and Identification of an Important Pigment, Sepiapterin, from Integument of thelemonMutant of the Silkworm,Bombyx mori

Studies on the genetical and biochemical properties of the Kiuki mutant of the silkworm, Bombyx mori.

Pterine als Wirkstoffe und Pigmente

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