Genetic variations in some Egyptian Zea mexicana genotypes based on RAPD and AFLP markers

Radwan, Khaled; Abdel-Fattah, G.; Badawi, M. A.; Zayed, E. M.; Tarrd, M. M.; El-Baghdady, Manal; El-Maksoud, Reem M. Abd

doi:10.21608/jbaar.2021.177577

Cited by 4 publications

(5 citation statements)

References 43 publications

(59 reference statements)

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“…Data presented in Table (1) showed that there are significant differences at 0.05 probability levels among the seven genotypes over the two seasons and the three cuts for all studied traits i.e., plant height, stem diameter, fresh yield, dry yield and their total fresh and dry yields. These results were in line with those of Wright (1935), Radwan and Zayed (2021) and El-Gaafarey et al (2023).…”

Section: Results and Discussion A-forage Experiment: Analysis Of Vari...supporting

confidence: 90%

Enhancement the productivity of teosintina, Millet and Sourgham crops for resistance to common smut and downy mildew diseases

El-Gaafarey

Elsayed

El-Nahrawy

2023

EBSS

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O STUDY correlation and path analysis for yield and its attributing characters ten forage and silage summer forage crops were evaluated at Sakha Agricultural Research Station in (Northern Delta) A.R.C., Egypt, 2020 and 2021 summer seasons. the studied crops were seven summer forage crops (three cuts) and three summer forage crops (only one cut). Highly significant differences were detected among the seven genotypes over the two seasons for plant height, stem diameter, fresh yield, dry yield at the three cuts over two seasons and their total fresh and dry yields. Highly significant differences were found among the three crops for silage fresh, dry yield and its components in the combined analysis. Correlation coefficients were highly significant in positive direction between fresh forage yield cut 1, 2, 3, total fresh yield, dry yield cut 1, 2, 3, total dry yield, plant height cut 1, 2, 3, stem diameter cut 1, 2 and cut 3 implying the effectiveness of these traits in selection for fresh forage yield. In addition, insignificant negative estimates of correlation coefficients observed between fresh forage yield cut 1, 2, 3, total fresh yield, dry yield cut 1,2,3, total dry yield, plant height cut 1,2,3, stem diameter cut 1, 2 and cut 3 and each of fresh leaf stem percent cut 1, 2, 3, LI%, SI and TI% traits, indicating that selection of low LI%, SI and TI% contribute to high estimates of these characters. Highly significant and negative estimates of correlation coefficients observed among fresh leaf stem percent cut 1, 2, 3 with LI%, SI and TI%. Total infection % (TI%) had positive and highly significant correlation with dry yield cut 1, 2, 3, total dry yield, plant height cut 1, 2, 3, stem diameter cut 1, 2, fresh leaf stem percent cut1, 2 and fresh leaf/stem percent cut 3. Meanwhile, relationship among total fresh forage yield with local infection % (LI %), systemic infection % (SI %) and total infection % (TI %) were insignificant and negative, meaning presence of non-influential losses in yield because most genotypes were resistant.

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Section: Results and Discussion A-forage Experiment: Analysis Of Vari...supporting

confidence: 90%

Enhancement the productivity of teosintina, Millet and Sourgham crops for resistance to common smut and downy mildew diseases

El-Gaafarey

Elsayed

El-Nahrawy

2023

EBSS

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“…They constructed a dendrogram in which 3 main clusters emerged. On the other hand, the authors Radwan et al (2021), Al-Obaidi et al (2018, Ristic et al (2013) and Sharawy et al (2011) detected a higher number of DNA fragments per primer and a higher percentage of polymorphism. Radwan et al (2021) examined the genetic diversity of 16 Zea mexicana populations.…”

Section: Discussionmentioning

confidence: 92%

“…On the other hand, the authors Radwan et al (2021), Al-Obaidi et al (2018, Ristic et al (2013) and Sharawy et al (2011) detected a higher number of DNA fragments per primer and a higher percentage of polymorphism. Radwan et al (2021) examined the genetic diversity of 16 Zea mexicana populations. 14 RADP primers amplified 141 DNA fragments (average of 10.1 bands per primer) out of which, 102 (72.3%) were polymorphic.…”

Section: Discussionmentioning

confidence: 92%

“…Balážová ( 2016) determined lower values of MI and DDI using the RAPD technique (MI = 5.66; DI = 1.84) and SCoT (MI = 4.21; DDI = 2.11) compared to our results, however, she considered RAPD to be the most effective technique for determining maize diversity. Radwan et al (2021) compared the efficiency of RAPD and AFLP markers in the analysis of the Zea mexicana genome and found that the marker index (MI) of RAPD (1.1) was significantly lower compared to AFLP (11.21). Other authors used MI and DDI to evaluate the polymorphism and diversity analyzes in other cereals.…”

Section: Discussionmentioning

confidence: 99%

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Genetic diversity of maize resources revealed by different molecular markers

Chňapek,

Špaleková,

Gálová

et al. 2023

Preprint

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Maize (Zea mays L.) is the third most important cereal crop in the world because of its nutritional value and industrial benefits. Molecular markers are used mainly by the breeders to study the genetic variability of genotypes and its application in the breeding process. Two types of molecular markers, 10 RAPD primers and 10 SCoT primers, were assayed to determine the genetic diversity of 25 Slovak maize lines and 25 maize varieties. A high level of polymorphism was found with both RAPD and SCoT markers, which was confirmed by high average PIC values using both techniques. The efficiency of individual marker techniques in the detection of genotype diversity can be compared by calculating the marker index (MI) and detecting diversity index (DDI). A higher MI (11.788) and DDI (2.358) value was achieved by the SCoT technique compared to the RAPD method. Three joint dendrograms and PCoA plots constructed based on RAPD, SCoT and both methods combined confirmed the unambiguous separation of maize lines and varieties from each other. The results obtained from the RAPD and SCoT analysis can be used for the selection of potentially suitable biological sources for further marker-assisted breeding.

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“…Data presented in Table 2 showed that there are highly significant differences among the seven genotypes at the first and second seasons (2020/2021) and the combined for all studied traits i.e plant height , stem diameter , fresh yield , dry yield at the three cuts in the two seasons and their total fresh and dry yields [18,19,20,21,22].…”

Section: Results and Dissectionmentioning

confidence: 99%

Assessing of Some Summer Forage Crops to Infection by Common Smut and Downy Mildew Diseases for Forage and Silage Production

El-Gaafarey

Elsayed²,

El-Nahrawy³

2023

JAERI

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The present study was carried out at Sakha Agricultural Research Station in the summer 2020 and 2021 seasons. Ten summer forage crops namely, Millet Saudia Arabia, Selection Millet1, Selection Millet2, Millet Shandawel 1, Sudangrass Giza1, Sudangrass Piper black ,Sorghum SX-17 were evaluated for downy mildew and common smut diseases and forage yield and some traits. Three cuts were taken from the seven fresh forage crops and only one cut for silage from maize (95 days), Teosinte and maize × teosinte (100days). Mean values of forage crops genotypes revealed significant to highly significant differences. Piper black had the highest fresh and dry forage yields at the three cuts and total over the two seasons which had (26.4,22.3,17.1 and 65.7kg/plot) for fresh and 5.3,2.8,2.5 and 10.59 for dry yield ,respectively . For combined data millet Shandawal 1 had 46.7 kg/plot, and 7.8 kg/plot ,while Saudia Arabia Millet gave 31.3 and 4.9 kg/plot, for total fresh and dry forage yields, respectively, so Shandawal 1 surpass Saudia Arabia by 49.2 and 59.2% for fresh and dry yield . Also, sorghum, piper black surpass SX-17 by 19.2 and 26.5 % for total fresh and dry yield, respectively. Maize SC168 x Teosinte Sakha was the highest for plant height (298 cm) ,stem diameter (2.9 cm) ,fresh/leaf stem percent(81%) and fresh forage yield (69 kg/plot) and exceed teosinte by 5.7,93.3,24.6 and 8.7% ,respectively . Meanwhile, exceed maize SC168 by 11.2, 16.0, 72.3 and 122.6%for plant height ,stem diameter ,fresh/leaf stem percent and fresh forage yield ,respectively. Saudia Arabia Millet and hybrid SX -17 were susceptible (s) to downy mildew disease on the other hand pearl millet 2,3,Giza 1 and piper black were resistance ( R ) to downy mildew disease at the three cuts in the two seasons . The local pearl millet1 , pearl millet2 , and pearl millet 3 (Shandawal 1) recorded the lowest percent of total infection by downy mildew disease1.666,0.000 and 1.333% respectively, also Giza 1 and piper black genotypes recorded the lowest percent of total infection by downy mildew disease which had 2.13 and 4.13% ,respectively. These tested local pearl millet and Giza 1 and piper black , were resistant(R) to downy mildew disease and can be used in breeding programs as sources of resistance to tested disease, In the revers , the imported pearl millet (Saudia Arabia pearl millet) and hybrid sorghum SX -17 were susceptible and recorded 20.33and 34.26, respectively. These results indicated dangerousness of importing susceptible materials. On the other hand, teosinte genotype was resistant to infection by downy mildew disease and the total infection percent was 0.000 in the first reading and 0.666% in the second , but maize was susceptible and recorded (16.70%) as total infection. Therefore the crossing between maize and teosinte resulted in moderate resistant (MR) whereas, 5.966% and 5.166% in the first and second reading. As common smut, teosinte was susceptible (s) but maize and maize x teosinte hybrid were moderate resistant (MR) for common smut infection disease. Finely, the local genotypes are better than the imported Saudi Arabia millet. Hybrid maize x teosinte was the best had more than twice maize silage yield (122.6%).

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Genetic variations in some Egyptian Zea mexicana genotypes based on RAPD and AFLP markers

Cited by 4 publications

References 43 publications

Enhancement the productivity of teosintina, Millet and Sourgham crops for resistance to common smut and downy mildew diseases

Enhancement the productivity of teosintina, Millet and Sourgham crops for resistance to common smut and downy mildew diseases

Genetic diversity of maize resources revealed by different molecular markers

Assessing of Some Summer Forage Crops to Infection by Common Smut and Downy Mildew Diseases for Forage and Silage Production

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