Genetic variations and haplotypic diversity in the Myostatin gene of New Zealand cattle breeds

Haruna, Ishaku L.; Ekegbu, Ugonna J.; Ullah, Farman; Najafabadi, Hamed Amirpour; Zhou, Huitong; Hickford, Jonathan G. H.

doi:10.1016/j.gene.2020.144400

Cited by 6 publications

(10 citation statements)

References 37 publications

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“…All seven of the nucleotide substitutions identified in this study have been previously reported in a study of NZ cattle breeds, which included; Hereford, Angus, Shorthorn, Charolais, Red Poll, South Devon, Simmental, Murray Grey, HF × J cross cattle, and some composite breeds [19]. The nucleotide substitutions c.373+751G/T, c.373+803T/G, c.373+877A/G, c.373+895G/C, and c.374−909C/T were identified in all the ten aforementioned breeds, while c.374−842G/C was found in all but four breeds (Red Poll, Shorthorn, Simmental, and Composites breeds) and the c.374−812A/G was only found in Shorthorn and HF × J cross breeds.…”

Section: Discussionsupporting

confidence: 54%

“…The PCR-SSCP analyses coupled with DNA sequencing revealed five banding patterns (A-E) in the region of intron 1 investigated (Figure 1). A total of seven single-nucleotide substitutions (c.373+751G/T, c.373+803T/G, c.373+877A/G, c.373+895G/C, c.374−909C/T, c.374−842G/C, c.374−812A/G) were identified, all of which have been previously reported [19].…”

Section: Identification Of Nucleotide Sequence Variation In Bovine Mstnmentioning

confidence: 56%

“…The intron 1 region of MSTN was amplified using forward and reverse primers (5 -catggtactattgttgagag-3 and 5 -aaggcaaatctattccagg-3 respectively) adapted from the work of Haruna et al [19]. The 15-µL reactions contained the purified DNA on a 1.2-mm diameter disc of the FTA™ card, and a content of 0.25 µM for each primer, 150 µM for each dNTP (Eppendorf, Hamburg, Germany), 3.0 mM Mg 2+ , 0.5 U of Taq DNA polymerase (Qiagen, Hilden, Germany), and 1× the buffer supplied with the DNA polymerase enzyme.…”

Section: Polymerase Chain Reaction (Pcr) Amplification Of a Region Ofmentioning

confidence: 99%

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Associations between the Bovine Myostatin Gene and Milk Fatty Acid Composition in New Zealand Holstein-Friesian × Jersey-Cross Cows

Haruna

Ekegbu

et al. 2020

Animals

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The myostatin gene (MSTN), which encodes the protein myostatin, is pleiotropic, and its expression has been associated with both increased and decreased adipogenesis and increased skeletal muscle mass in animals. In this study, the polymerase chain reaction, coupled with single strand conformation polymorphism analysis, was utilized to reveal nucleotide sequence variation in bovine MSTN in 410 New Zealand (NZ) Holstein-Friesian × Jersey (HF × J)-cross cows. These cows ranged from 3 to 9 years of age and over the time studied, produced an average 22.53 ± 2.18 L of milk per day, with an average milk fat content of 4.94 ± 0.17% and average milk protein content of 4.03 ± 0.10%. Analysis of a 406-bp amplicon from the intron 1 region, revealed five nucleotide sequence variants (A–E) that contained seven nucleotide substitutions. Using general linear mixed-effect model analyses the AD genotype was associated with reduced C10:0, C12:0, and C12:1 levels when compared to levels in cows with the AA genotype. These associations in NZ HF × J cross cows are novel, and they suggest that this variation in bovine MSTN could be explored for increasing the amount of milk unsaturated fatty acid and decreasing the amount of saturated fatty acid.

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Section: Discussionsupporting

confidence: 54%

Section: Identification Of Nucleotide Sequence Variation In Bovine Mstnmentioning

confidence: 56%

Section: Polymerase Chain Reaction (Pcr) Amplification Of a Region Ofmentioning

confidence: 99%

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Associations between the Bovine Myostatin Gene and Milk Fatty Acid Composition in New Zealand Holstein-Friesian × Jersey-Cross Cows

Haruna

Ekegbu

et al. 2020

Animals

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“…Unfortunately, the primers used in this study did not cover these loci. Haruna et al [14] found seven nucleotide variations (c.373+751G>T, c.373+803 T>G, c.373+877A>G, c.373+895G>C, c.374-909C>T, c.374-842G>C, c.374-812A>G) in intron 1 of MSTN in New Zealand cattle breeds, of which two variants (c.748-281C>G and c.748-352C>T) have been reported in Qinchuan and Red Angus cattle [11]. In contrast to the results of this study, Haruna et al [14] did not observe any nucleotide variation in exon 1 of the MSTN gene in New Zealand cattle.…”

Section: Discussionmentioning

confidence: 99%

“…Mutations in the MSTN gene can affect the proper regulation of skeletal muscle mass, which is important for improved meat production traits. Many previous studies have investigated genetic variations of the MSTN gene not only in cattle [3,6,[10][11][12][13][14], but also in other livestock species, including goats [15], geese [16], chickens [17], and sheep [18]. Several cattle breeds are characterized by a double-muscling (DBM) phenotype, which increases muscle mass due to loss-of-function mutations in bovine MSTN [3,10,19,20].…”

Section: Introductionmentioning

confidence: 99%

Genetic variation in the first intron and exon of the myostatin gene in several Indonesian cattle populations

Prihandini¹,

Primasari²,

Aryogi³

et al. 2021

Vet World

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Background and Aim: Myostatin (MSTN), a member of the transforming growth factor-β family, is a negative regulator of muscle mass. This study aimed to detect the genetic variation of the 1160 bp fragment of exon 1 and part of intron 1 of the MSTN gene in several cattle populations raised in Indonesia. Materials and Methods: Polymerase chain reaction products of the MSTN gene amplified from 92 animals representing 10 cattle populations (Peranakan Ongole [PO], Belgian Blue x PO cross, Rambon, PO x Bali cross, Jabres, Galekan, Sragen, Donggala, Madura, and Bali) were sequenced, compared, and aligned with bovine MSTN of Bos taurus (GenBank Acc. No. AF320998.1) and Bos indicus (GenBank Acc. No. AY794986.1). Results: Four nucleotide substitutions (nt 1045 and 1066 in intron 1; nt 262 and 418 in exon 1) and two indels (nt 807 and 869 in intron 1) were synonymous mutations. Among these substitutions, only the nt 262G>C and nt 418A>G loci were polymorphic in all populations, except Bali cattle. The frequencies of the nt 262C (0.82) and nt 418A (0.65) alleles were highest. For the nt 262G>C locus, the CC genotype had the highest frequency (0.66) followed by GC (0.30) and CC (0.03). For the nt 418A>G locus, the AG genotype had the highest frequency (0.52) followed by AA (0.39) and GG (0.09). Conclusion: The results, showing genetic variations in exon 1 and intron 1 of the MSTN gene, might be helpful for future association studies.

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Associations between polymorphisms in the myostatin gene with calving difficulty and carcass merit in cattle

Ryan,

Purfield,

Naderi

et al. 2023

Journal of Animal Science

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A fully functional myostatin gene inhibits muscle fiber growth. The objective of the present study was to quantify the association between 21 known myostatin mutations with both calving and carcass traits in 12 cattle breeds. The myostatin genotypes of 32,770 dam-progeny combinations were used in the association analysis of calving dystocia, with the genotypes of 129,803 animals used in the mixed model association analyses of carcass weight, conformation, and fat score. The mixed model included additive genetic, maternal, and permanent environmental effects where appropriate. The mutant genotypes of nt821, Q204X, and F94L were all associated (P < 0.01) with more calving difficulty when present in either the dam or the progeny. The nt821 deletion had the greatest association with calving difficulty when the homozygous deletion was present in either the calf (0.37 points greater calving difficulty score relative to calves carrying no copies of the deletion based on a one to four scale) or the dam (1.30 points greater calving difficulty score relative to dams carrying no copies of the deletion), although the association between the calf’s nt821 genotype and calving difficulty differed depending on the nt821 genotype of the dam. With the exception of nt748_78, nt414, and nt374_51, all other seven segregating myostatin variants were associated (range of allele substitution effect size relative to animals with no copies of the mutant allele) with carcass weight (2.36 kg lighter to 15.56 kg heavier), all 10 segregating variants with conformation (0.15 units less conformed to 2.24 units more conformed assessed on a scale of 1 to 15), and all segregating variants other than E226X with carcass fat (0.23 units less carcass fat cover to 3.85 units more carcass fat cover assessed on a scale of 1 to 15). Of these, the F94L, Q204X, and nt821 mutations generally had the greatest association with all three carcass traits, giving rise to heavier and more conformed carcasses. Despite the antagonistic genetic relationship between calving difficulty and carcass traits, the nt374_51, F94L, and E226X mutations were all associated with improved carcass merit while having minimal expected consequences on calving difficulty. Thus, animals carrying these mutation(s) may have favorable genetic merit for calving difficulty and carcass merit. Furthermore, depending on the dam genotype, a bull with two copies of the nt821 mutation can produce progeny with improved carcass merit while minimizing calving problems.

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Genetic variations and haplotypic diversity in the Myostatin gene of New Zealand cattle breeds

Cited by 6 publications

References 37 publications

Associations between the Bovine Myostatin Gene and Milk Fatty Acid Composition in New Zealand Holstein-Friesian × Jersey-Cross Cows

Associations between the Bovine Myostatin Gene and Milk Fatty Acid Composition in New Zealand Holstein-Friesian × Jersey-Cross Cows

Genetic variation in the first intron and exon of the myostatin gene in several Indonesian cattle populations

Associations between polymorphisms in the myostatin gene with calving difficulty and carcass merit in cattle

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