Genetic variation of some isolates of Cladosporium sphaerospermum isolated from different environments

Abstract

The study was carried out, in the laboratory of Plant Virology, Plant Protection Department, College of Agriculture, University of Kerbala, to isolate and identify six isolates of the fungus Cladosporium sphaerospermum isolated from different environments (seeds, air, plant residues, and soil) in Al-Najaf province, Iraq. The fungal isolates were identified using the polymerase chain reaction (PCR) and determining the nucleotide sequence products of DNA using ITS1 and ITS4 primer pair. Results of analysis of the nucleotide sequences using BLAST (Basic Local Alignment Search Tool) showed that all the identified isolates of the fungus belong to C. sphaerospermum. A comparison of the nucleotide sequences with those available in the National Centre for Biotechnology Information (NCBI) revealed that all the identified C. sphaerospermum sequences were previously registered in NCBI.

Evaluating Mosquito Biocontrol Effectiveness by Isolating and Characterizing Some Fungi Against Culex Pipiens and Anopheles Stephensi in Sothern Part of Iraq

“…This fungus produces three types of the conidia are oval or lemon-shaped, namely Macroconidia, Intermediatconidia, and Microconidia (Figure 1). The results showed that the sequence of nitrogenous bases of study isolates, their number was 569 PB, the result was identical percentage (90%) with many global and local isolates found in the NCBI gene bank (Figure 2) which confirms and supports the validity of the phenotypic and microscopic diagnosis of this fungus (Kozel and Wickes, 2014;Abdullah et al, 2019).…”

“…Direct sequencing was done on the PCR products in both directions. Using the Basic Local Alignment Search Tool (BLAST), nucleotide sequences were aligned and compared with the sequences of the other fungal isolates that were accessible in the NCBI database (Abdullah et al, 2019). Using MEGA 11, phylogenetic analysis of all fungal nucleotide sequences was compared.…”

New Records in Iraq and Arab Nations for some Fungi Isolated from Al-Barakia wastewater treatment plant in Al-Najaf Province

“…The results shown in Table 8 comparing the series nitrogenous bases of the DNA double A.niger isolates (14,15) with the available Information in (NCBI) show that the highest genetic similarity rate of 100% with Previously isolated fungus isolates from China (KP764197), followed by the diagnosed fungus isolates in China (MT446087, MN429196) India (MN180811) with a similarity of 99% and also recorded in Egypt (LC582533)) with a similarity rate of 99%, The above Table It showed the proportion of similarity between this isolate and isolates from across the world that has been recorded (NCBI), where it was not recorded in Iraq.…”

“…This study aims to diagnose the fungal isolates (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) and those isolated from the Al-Barakia wastewater treatment plant by PCR. Therefore, the primer (ITS1 and ITS40) was delivered to the Macrogen Company of South Korea to determine The sequence of the nitrogenous bases.…”

“…After all, it is affected by environmental factors that may influence the shape and colors of spores, size and fungal colonies 12,13 . PCR technology is the molecular technique that relies on the selection and amplification of a specific area of the organism's genome, and thus knowledge of the genetic relationships in terms of similarity and the difference between the species of fungi that will help in the phenotypic diagnosis of the study fungi 14,15 . This study aimed to isolate and diagnose fungi isolates using (PCR), determine the sequence of nitrogenous bases, and identify similarities and differences in genetics between the isolates-compared with the same fungi internationally diagnosed recorded in ( NCBI).…”