New Records in Iraq and Arab Nations for some Fungi Isolated from Al-Barakia wastewater treatment plant in Al-Najaf Province

Abstract

This study was conducted in 2020 in the wastewater treatment plant in Al-Barakia Najaf, where samples were taken in November from the Bioshft unit and the compact unit from the plant to know the efficiency of the plant in treatment. The process of isolation and purification was Microbiology Laboratory_ Ecology and Pollution Department - College of Science - University of Kufa. Its diagnosis was carried out at the Plant Virus Laboratory - College of Agriculture - the University of Karbala, and (19) fungal isolates isolated from wastewater treatment plants were diagnosed. These isolates were analyzed using the PCR technique and determination of the nucleotide sequences of the polymerase chain reaction products that were amplified from those isolates using ITS4 and ITS1 primers. It belonged to the fungus A. caespitosus. and isolated A flavus (7,8), T. asperellum (9-11) A.tubingensis (12), A.terrus(13), A.niger (14,15), A. alternata (16), C.sphaerospermum (17), A.oryzae (18), Acremonium sp (19), similarity rate of 100% with isolates registered with the NCBI. The results show that all the isolated fungi diagnosed in this study are recorded globally. However, they are not recorded in Iraq, and (5) isolates are not recorded in the Arab nation and Iraq, including A. tubingensis, C.sphaerospermum, A. alternate, and A.oryzae, while the isolate Acremonium sp. According to the National Center for Biotechnology Information NCBI, it is registered only in Germany under the number (AJ557731), and the similarity rate is 100% with the isolate diagnosed in this study. Keywords: wastewater; Molecular Identification; fungi; Polymerase chain reaction (PCR); DNA sequence analysis.