Genetic variation of Sargassum horneri populations detected by inter-simple sequence repeats

Abstract

ABSTRACT. The seaweed Sargassum horneri is an important brown alga in the marine environment, and it is an important raw material in the alginate industry. Unfortunately, the fixed resource that was originally reported is now reduced or disappeared, and increased floating populations have been reported in recent years. We sampled a floating population and 4 fixed cultivated populations of S. horneri along the coast of Zhejiang, China. Inter-simple sequence repeat (ISSR) markers were applied in this research to analyze the genetic variation between floating populations and fixed cultivated populations of S. horneri. In total, 220 loci were amplified with 23 ISSR primers. The percentage of polymorphic loci within each population ranged from 53.64 to 95.45%. The highest diversity was observed in population 3, which was the local species that was suspension cultured in the lab and then fixed cultivated in the Nanji Islands before sampling. The lowest diversity was obtained in the floating population 4. The genetic distances among 620 J.R. Ren et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (1): 619-625 (2015) the 5 S. horneri populations ranged from 0.0819 to 0.2889, and the distance tendency confirmed the genetic diversity. The results suggest that the floating population had the lowest genetic diversity and could not be joined into the cluster branch of the fixed cultivated populations.

Impact of Different Geographical Locations on Genetic Variation and Phytochemical Constituents of Two Medicinal Marine Algae.

“…Also, SCoT-PCR technique was applied to numerous plant genetic studies as it overcomes most limitations of other markers. Molecular markers are predictable to create new awareness and improve our understanding of the genetics of complex traits (Ren et al, 2015).…”

Structural Features and Potent Antidepressant Effects of Total Sterols and β-sitosterol Extracted from Sargassum horneri

“…Sargassum is a large genus with more than 150 species, including Sargassum horneri , a brown seaweed found in the Northwestern Pacific Ocean and the adjoining seas of Korea, Japan, and China. Total steroid and β-sitosterol extracts from S. horneri have been used to treat scrofula, gall, goiter, and edema [ 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. However, the purification and characterization of these extracts and their antidepressant effects have not been well studied.…”