Genetic variation of natural and cultured stocks of Paralichthys olivaceus by allozyme and RAPD

You, Feng; Zhang, Peijun; Wang, Keling; Xiang, Jianhai

doi:10.1007/s00343-007-0078-9

Cited by 6 publications

(7 citation statements)

References 18 publications

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“…The primary focus of these studies was fl ounder populations found off the Japanese or Korean coasts. Several studies have investigated genetic variations in natural and cultured fl ounder populations along the Chinese coast; a loss of genetic diversity was reported in the cultured populations (You et al, 2001(You et al, , 2007Zhang et al, 2004;Song et al, 2011). However, the genetic diversity in the natural populations in the China seas was never resolved.…”

Section: Introductionmentioning

confidence: 95%

“…In recent years, the natural resources of Japanese fl ounder have been declining because of changes in the environment and increased fi shing pressure (Zhang et al, 2004;You et al, 2007). Moreover, aquaculture production of this species has dropped considerably over the last decade because of inbreeding depression and problems of viral and bacterial disease and despite extensive fi shery management efforts (Liu et al, 2005).…”

Section: Introductionmentioning

confidence: 98%

“…Molecular markers can be used effectively to estimate genetic variation and population structure in different populations, thus providing a basis for better management of whole populations and thence sustainable fi sheries (Liu and Cordes, 2004;Liu et al, 2009;Yue et al, 2009). Genetic studies have investigated the basic genetic variation in Japanese fl ounder using various molecular markers such as allozyme, RAPD, AFLP, microsatellites, and mitochondrial DNA (mtDNA) (You et al, 2001(You et al, , 2007Zhang et al, 2004;Liu et al, 2005;Kim et al, 2010;Song et al, 2011). Sekino and Hara (2001) analyzed the genetic divergence of fl ounder populations around the coastal sea areas of Japan using microsatellite markers.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity in two Japanese flounder populations from China seas inferred using microsatellite markers and COI sequences

Liu

et al. 2012

Chin. J. Ocean. Limnol.

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Japanese fl ounder is one of the most important commercial species in China; however, information on the genetic background of natural populations in China seas is scarce. The lack of genetic data has hampered fi shery management and aquaculture development programs for this species. In the present study, we have analyzed the genetic diversity in natural populations of Japanese fl ounder sampled from the Yellow Sea (Qingdao population, QD) and East China Sea (Zhoushan population, ZS) using 10 polymorphic microsatellite loci and cytochrome c oxidase subunit I (COI) sequencing data. A total of 68 different alleles were observed over 10 microsatellite loci. The total number of alleles per locus ranged from 2 to 9, and the number of genotypes per locus ranged from 3 to 45. The observed heterozygosity and expected heterozygosity in QD were 0.733 and 0.779, respectively, and in ZS the heterozygosity values were 0.708 and 0.783, respectively. Signifi cant departures from Hardy-Weinberg equilibrium were observed in 7 of the 10 microsatellite loci in each of the two populations. The COI sequencing analysis revealed 25 polymorphic sites and 15 haplotypes in the two populations. The haplotype diversity and nucleotide diversity in the QD population were 0.746±0.072 8 and 0.003 34±0.001 03 respectively, and in ZS population the genetic diversity values were 0.712±0.047 0 and 0.003 18±0.000 49, respectively. The microsatellite data ( F st =0.048 7, P <0.001) and mitochondrial DNA data ( F st =0.128, P <0.001) both revealed signifi cant genetic differentiation between the two populations. The information on the genetic variation and differentiation in Japanese fl ounder obtained in this study could be used to set up suitable guidelines for the management and conservation of this species, as well as for managing artifi cial selection programs. In future studies, more geographically diverse stocks should be used to obtain a deeper understanding of the population structure of Japanese fl ounder in the China seas and adjacent regions.

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Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity in two Japanese flounder populations from China seas inferred using microsatellite markers and COI sequences

Liu

et al. 2012

Chin. J. Ocean. Limnol.

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“…Kim et al (2010) also employed microsatellite markers to analyze the genetic structure of populations collected from coastal sea areas around Korea. Moreover, several studies have compared the genetic variation in hatchery populations to wild populations using random amplified polymorphic DNA (RAPD) (You et al, 2007), microsatellite markers (Sekino and Hara, 2001) and amplified fragment length polymorphism (AFLP) (Zhang et al, 2004). The genetic variation of selected stocks has also been reported (Sekino et al, 2002;Liu et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Genetic differentiation in Japanese flounder in the Yellow Sea and East China Sea by amplified fragment length polymorphism (AFLP) and mitochondrial DNA markers

Li¹,

Xu²,

Liu³

et al. 2011

Afr. J. Biotechnol.

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The population structure of Japanese flounder (Paralichthys olivaceus) in the Yellow and East China Seas were analyzed using amplified fragment length polymorphism (AFLP) and cytochrome c oxidase subunit I (COI) gene sequencing. A total of 390 reproducible bands were generated by 10 AFLP primer combinations in two populations collected from the coasts of Qingdao (located at the Yellow Sea) and Zhoushan (located at the East China Sea). The percentage of polymorphic loci (P), Nei's genetic diversity (H) and Shannon's information index (I) values were higher in the Qingdao population (P = 72.85%, H = 0.243 and I = 0.364) than those in the Zhoushan population (P = 56.35%, H = 0.189 and I = 0.284). The genetic diversity reduction in the Zhoushan population may be attributed to fishing pressure and habitat loss in this area. Based on the COI sequencing analysis, a total of 25 polymorphic sites were examined, and 15 haplotypes were identified in the two populations. The haplotype diversity (h) and nucleotide diversity (π) values in the Qingdao population were 0.746 ± 0.0728 and 0.00334 ± 0.00103, respectively. The corresponding values in the Zhoushan population were 0.712 ± 0.0470 and 0.00318 ± 0.00049. Both the AFLP and mtDNA data revealed significant genetic differentiation between the two populations. The present study discussed the factors that may result in genetic differentiation between the populations in the Yellow and East China Seas.

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“…RAPD-PCR amplifies DNA segments of variable lengths and such length polymorphisms are inherited in a Mendelian fashion and thus can be used as genetic markers [10]. RAPD analysis has been extensively used to evaluate genetic diversity in Brycon lundii, Astyanax altiparanae and Prochilodus marggravii [11][12][13], flounder [14], Labeo rohita [15], catfish [16,17], mud eel [18], ornamental reef fishes [19], crocodile [20], black rat snake [21], and hilsa [22]; and in subspecies identification of tilapia [23], Badis badis, Dario dario [24], and lamprey [25]. A very limited genomic research has been carried out in Badis badis species till date and insufficient genomic information is available to perform other sophisticated fingerprinting techniques where whole genomic sequence is necessary; therefore, RAPD fingerprinting is the best choice for genetic diversity analysis in this species.…”

Section: Introductionmentioning

confidence: 99%

Study of the Genetic Diversity of the Ornamental FishBadis badis(Hamilton-Buchanan, 1822) in the Terai Region of Sub-Himalayan West Bengal, India

Mukhopadhyay

Bhattacharjee

2014

International Journal of Biodiversity

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Dwarf chameleon fish or Badis badis, a lesser known ornamental freshwater fish, has recently been included in the Indian threatened category of fish list. There are insufficient studies with regard to the assessment of genetic background of this ichthyofauna, especially in the western sub-Himalayan region of West Bengal, India, popularly known as the Terai. The present study is the first attempt to investigate the present status of the genetic background of this species in the Mahananda and Balason rivers, major streams of this region. Twenty-one selective RAPD primers generated 53 and 60 polymorphic fragments in the Mahananda and Balason populations, respectively. The proportion of polymorphic loci, Nei's genetic diversity (H), and Shannon's index ( ) were 0.4416, 0.1654 ± 0.2023, and 0.2450 ± 0.2907, respectively, in Mahananda river population and were 0.5041, 0.1983 ± 0.2126, and 0.2901 ± 0.3037, respectively, in Balason river population. Inbreeding coefficient and degree of gene differentiation were also calculated. The H and were found to be 0.1601±0.1944 and 0.2363±0.2782, respectively, in overall Mahananda-Balason river system. Our study revealed considerable lack of genetic variation among the individuals of Badis badis. The genetic data obtained from the present study lend support to the view that there is a scope of stock improvement for this ichthyofauna.

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Genetic variation of natural and cultured stocks of Paralichthys olivaceus by allozyme and RAPD

Cited by 6 publications

References 18 publications

Genetic diversity in two Japanese flounder populations from China seas inferred using microsatellite markers and COI sequences

Genetic diversity in two Japanese flounder populations from China seas inferred using microsatellite markers and COI sequences

Genetic differentiation in Japanese flounder in the Yellow Sea and East China Sea by amplified fragment length polymorphism (AFLP) and mitochondrial DNA markers

Study of the Genetic Diversity of the Ornamental FishBadis badis(Hamilton-Buchanan, 1822) in the Terai Region of Sub-Himalayan West Bengal, India

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