Study of the Genetic Diversity of the Ornamental FishBadis badis(Hamilton-Buchanan, 1822) in the Terai Region of Sub-Himalayan West Bengal, India

Abstract

Dwarf chameleon fish or Badis badis, a lesser known ornamental freshwater fish, has recently been included in the Indian threatened category of fish list. There are insufficient studies with regard to the assessment of genetic background of this ichthyofauna, especially in the western sub-Himalayan region of West Bengal, India, popularly known as the Terai. The present study is the first attempt to investigate the present status of the genetic background of this species in the Mahananda and Balason rivers, major streams of this region. Twenty-one selective RAPD primers generated 53 and 60 polymorphic fragments in the Mahananda and Balason populations, respectively. The proportion of polymorphic loci, Nei's genetic diversity (H), and Shannon's index ( ) were 0.4416, 0.1654 ± 0.2023, and 0.2450 ± 0.2907, respectively, in Mahananda river population and were 0.5041, 0.1983 ± 0.2126, and 0.2901 ± 0.3037, respectively, in Balason river population. Inbreeding coefficient and degree of gene differentiation were also calculated. The H and were found to be 0.1601±0.1944 and 0.2363±0.2782, respectively, in overall Mahananda-Balason river system. Our study revealed considerable lack of genetic variation among the individuals of Badis badis. The genetic data obtained from the present study lend support to the view that there is a scope of stock improvement for this ichthyofauna.

Genetic Diversity and Population Structure Analyses of Threatened Amblyceps mangois from Sub-Himalayan West Bengal, India Through Rapd and ISSR Fingerprinting

“…163, 0.198±0.136 and 0.216±0.138, and Shannon's information indices were 0.403±0.03, 0.327±0.03 and 0.354±0.02 in Chalan beel, Mohanganj haor and Kangsha river, respectively (Tamanna et al, 2012). Another separate study of ours revealed that the Nei's genetic diversity (H) of Badis badis Mahananda and Balason river population was 0.1654 and 0.1983, respectively, and the Shannon's information index (H´) was calculated to be 0.2450 ± 0.2907 in the Mahananda river and 0.2901 ± 0.3037 in the Balason river (Mukhopadhyay and Bhattacharjee, 2014a), and the Shannon's information index ranged from 0.1648 ± 0.2691 to 0.2205 ± 0.2950 in the Terai region of West Bengal India (Mukhopadhyay and Bhattacharjee, 2015). In a different study on Barilius barna isolated from the Teesta river, it was found that the Nei's genetic diversity ranged from 0.172 ± 0.189 to 0.293 ± 0.164 and the Shannon's information index (I) ranged from 0.265 ± 0.268 to 0.445 ± 0.220 (Paul et al, 2016).…”

“…In our earlier study with Badis badis the proportion of polymorphic loci varies from 0.4371 (43.71%) in overall population to 0.6733 (67.33%) in between populations (Mukhopadhyay and Bhattacharjee, 2014a). In the present study, it was found that the individual population polymorphism was between lower and moderate in the Teesta river system (23.40% in ADR-3 to 35.46% in ADR-6), moderate in the Mahananda river system (28.37% in ATR-2 to 34.04% in ATR-3 population) and moderate in the Jaldhaka river system (39.72% in ADR-8 to 43.26% in ADR-11 population) (Tables 2, 3, 4).…”

Morphological and Genetic variations in the natural populations of Carinotetraodon travancoricus

“…Identifying populations through morphometric and meristic approaches has long been adopted towards achieving this objective (Almeida et al., 2008; Siddik et al., 2016). Conservation and management of wild populations could be performed by understanding their genetic variability (Mukhopadhyay & Bhattacharjee, 2014). Valdez‐Pineda et al.…”

10 Genetic diversity of loach Misgurnus anguillicaudatus (Cantor, 1842) in Vietnam by randomly amplified polymorphic DNA analysis

“…RAPD is a simple, easy technique for population genetic studies. RAPD markers have been widely used for analysis of genetic diversity of various aquatic organisms: Malaysian river catfish 7 ; silver crucian carp 37 ; cupped oysters 15 ; fishes Pimelodus maculatus, Prochilodus lineatus, Salminus brasiliensis and Steindachneridion scripta 25 ; Indian coldwater fishes 28 ; tilapia 4,5 ; common tiger prawn 23 ; giant freshwater prawn 29 ; freshwater mud eel 18 ; ornamental gold fish 24 ;ornamental fish Badis badis 20 ; fishes Labeo rohita, Catla catla and Cirrhina mrigala 21 ;square head climb perch 32 ; loach M. anguillicaudatus 1,6,19,35 , and other marine and freshwater fishes such as striped red mullet (Mullus surmuletus L.), Atlantic coastal striped bass (Morone saxatilis), freshwater crayfish (Austropotamobius pallipes), Asian arowana (Scleropages formosus), etc. 1 .…”